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Moradi, N. (author), Lopez Vazquez, C.M. (author), Hernandez, Hector Garcia (author), Proskynitopoulou, Vera (author), Vouros, Anastasios (author), Garagounis, Ioannis (author), Lorentzou, Souzana (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author)In recent decades, there has been a growing focus on the management and valorization of digestate, primarily driven by its nutrient-rich composition, which positions it as a promising resource for biofertilizer production. However, several countries still restrict the direct application of digestate due to its potential environmental hazard,...journal article 2024
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Nouri, Amir (author), Zinatizadeh, Ali Akbar (author), Zinadini, Sirus (author), van Loosdrecht, Mark C.M. (author)This study focuses on the development of an air-lift bio-electrochemical reactor (ALBER) with a continuous feeding regime. The objective is to enhance nitrogen removal from synthetic wastewater with a low carbon-to-nitrogen (C/N) ratio. The chemical oxygen demand (COD) and total nitrogen (TN) of the influent wastewater were 500 and 200 mg/L,...journal article 2024
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Surkatti, Riham (author), van Loosdrecht, Mark C.M. (author), Hussein, Ibnelwaleed A. (author), El-Naas, Muftah H. (author)This study investigates the development of polyvinyl alcohol (PVA) gel matrices for biomass immobilization in wastewater treatment. The PVA hydrogels were prepared through a freezing–thawing (F-T) cross-linking process and reinforced with high surface area nanoparticles to improve their mechanical stability and porosity. The PVA/nanocomposite...journal article 2024
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Doloman, Anna (author), de Bruin, S. (author), van Loosdrecht, Mark C.M. (author), Sousa, Diana Z. (author), Lin, Y. (author)Glycans are crucial for the structure and function of anaerobic granular sludge in wastewater treatment. Yet, there is limited knowledge regarding the microorganisms and biosynthesis pathways responsible for glycan production. In this study, we analysed samples from anaerobic granular sludges treating papermill and brewery wastewater,...journal article 2024
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Páez Watson, T.W. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)With the rapid growing availability of metagenome assembled genomes (MAGs) and associated metabolic models, the identification of metabolic potential in individual community members has become possible. However, the field still lacks an unbiassed systematic evaluation of the generated metagenomic information to uncover not only metabolic...journal article 2024
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Chen, L.M. (author), Beck, Paula (author), van Ede, J.M. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Anionic polymers, such as heparin, have been widely applied in the chemical and medical fields, particularly for binding proteins (e.g., fibroblast growth factor 2 (FGF-2) and histones). However, the current animal-based production of heparin brings great risks, including resource shortages and product contamination. Recently,...journal article 2024
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Hülsen, Tim (author), Venturato, Daniel (author), Chan, Clement (author), Vandi, Luigi (author), Laycock, Bronwyn (author), Pratt, Steven (author), Stegman, Samuel (author), van Loosdrecht, Mark C.M. (author), Batstone, Damien J. (author)Anoxygenic purple phototrophic bacteria (PPB) were utilised in an 80 L biofilm flat plate photobioreactor to generate polyhydroxyalkanoate (PHA) over 44 cycles, with acetate as feed. Over the cycles, net PHA yield (growth + accumulation) averaged 21% while accumulation yield averaged 55%. Average PHA content was 35 wt% volatile solids (VS),...journal article 2024
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Strubbe, Laurence (author), van Dijk, E.J.H. (author), Carrera, Paula (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)The aerobic granular sludge (AGS) process treats wastewater with a significantly lower footprint and energy consumption compared to conventional activated sludge systems. Nevertheless, there is still potential for optimizing its performance, and mathematical models are most valuable tools to this end. Aeration energy consumption deserves...journal article 2024
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Policastro, G. (author), Ebrahimi, S. (author), Weissbrodt, D.G. (author), Fabbricino, Massimiliano (author), van Loosdrecht, Mark C.M. (author)A dual-growth-limited continuous operated bioreactor (chemostat) was used to enhance lipid accumulation in an enrichment culture of microalgae. The light intensity and nitrogen concentration where both limiting factors resulting in high lipid accumulation in the mixed culture. Both conditions of light and nitrogen excess and deficiency were...journal article 2024
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Deng, Shaoyu (author), Liu, J. (author), Yang, Xiaofan (author), Sun, Dezhi (author), Wang, Aijie (author), van Loosdrecht, Mark C.M. (author), Cheng, Xiang (author)The accumulation of phosphorus in activated sludge in wastewater treatment plants (WWTPs) provides potential for phosphorus recovery from sewage. This study delves into the potential for releasing phosphorus from waste activated sludge through two distinct treatment methods—thermal hydrolysis and pH adjustment. The investigation was conducted...journal article 2024
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Ruiz Hadad, L. (author), Ali, Muhammad (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Saikaly, Pascal E. (author)Currently, the most cost-effective and efficient method for phosphorus (P) removal from wastewater is enhanced biological P removal (EPBR) via polyphosphate-accumulating organisms (PAOs). This study integrates a literature review with genomic analysis to uncover the phylogenetic and metabolic diversity of the relevant PAOs for wastewater...review 2024
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Chen, Le Min (author), Erol, Özlem (author), Choi, Young Hae (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Currently, there is a growing interest in transforming wastewater treatment plants (WWTPs) into resource recovery plants. Microorganisms in aerobic granular sludge produce extracellular polymeric substances (EPS), which are considered sustainable resources to be extracted and can be used in diverse applications. Exploring applications in other...journal article 2024
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Xevgenos, Dimitris (author), Tourkodimitri, K. Panteleaki (author), Mortou, M. (author), Mitko, K. (author), Sapoutzi, D. (author), Stroutza, D.E. (author), Turek, M. (author), van Loosdrecht, Mark C.M. (author)Circular economy has become a popular subject, attracting attention from academics, practitioners, and policy-makers alike. However, despite the excitement surrounding it, the concept of circular economy has been criticized for being vague and having multiple interpretations from different fields. As a result, there is a lack of evidence and...journal article 2024
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Rahimi, Zahra (author), Zinatizadeh, Ali Akbar (author), Zinadini, Sirus (author), van Loosdrecht, Mark C.M. (author), JBatstone, Damien (author)In this work, a one-stage dual internal circulation airlift anaerobic/anoxic/aerobic (DCAL-A2O) bioreactor was continuously operated for concurrent removal of nutrients and organics from milk processing wastewater (MPW). Special configuration of the airlift A2O bioreactor created possibility of the formation of desired anaerobic, anoxic and...journal article 2024
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Calderon Franco, D. (author), Corbera Rubio, F. (author), Cuesta Sanz, M. (author), Pieterse, Brent (author), de Ridder, David (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author), Laureni, M. (author), Weissbrodt, D.G. (author)Drinking water treatment plants (DWTPs) are designed to remove physical, chemical, and biological contaminants. However, until recently, the role of DWTPs in minimizing the cycling of antibiotic resistance determinants has got limited attention. In particular, the risk of selecting antibiotic-resistant bacteria (ARB) is largely overlooked in...journal article 2023
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Ochs, Pascal (author), Martin, Ben (author), Germain-Cripps, Eve (author), Stephenson, Tom (author), van Loosdrecht, Mark C.M. (author), Soares, Ana (author)Over the past twenty years, various commercial technologies have been deployed to remove ammonia (NH<sub>4</sub>–N) from anaerobic digestion (AD) liquors. In recent years many anaerobic digesters have been upgraded to include a pre-treatment, such as the thermal hydrolysis process (THP), to produce more biogas, increasing NH<sub>4</sub>–N...journal article 2023
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Strubbe, Laurence (author), van Dijk, E.J.H. (author), Deenekamp, Pascalle J.M. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)In the pursuit of reducing carbon footprint and in view of increasing energy prices, energy efficiency is more important than ever before. Batch-wise operated aerobic granular sludge reactors consume up to 50% less energy compared to conventional activated sludge systems because pumping energy is reduced and mixing equipment is not needed....journal article 2023
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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Cingoz, Seyma (author), Yoruk, Mustafa (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author), Ersahin, Mustafa Evren (author)Achieving a neutral/positive energy balance without compromising discharge standards is one of the main goals of wastewater treatment plants (WWTPs) in terms of sustainability. Aerobic granular sludge (AGS) technology promises high treatment performance with low energy and footprint requirement. In this study, high-rate activated sludge (HRAS...journal article 2023
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Ziliani, Agustina (author), Bovio-Winkler, Patricia (author), Cabezas, Angela (author), Etchebehere, Claudia (author), Garcia, Hector A. (author), López-Vázquez, Carlos M. (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Rubio-Rincón, Francisco J. (author)Ca. Accumulibacter was the predominant microorganism (relative FISH bio-abundance of 67 ± 5%) in a lab-scale sequential batch reactor that accomplished enhanced biological phosphorus removal (EBPR) while using glucose and acetate as the carbon sources (1:1 COD-based ratio). Both organic compounds were completely anaerobically consumed. The...journal article 2023
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Hu, Zhi Yi (author), Lin, Yi Peng (author), Wang, Qing Ting (author), Zhang, Yi Xin (author), Tang, Jie (author), Wang, Shuai (author), van Loosdrecht, Mark C.M. (author), Zeng, Raymond Jianxiong (author), Zhang, Fang (author)By maintaining the cell integrity of waste activated sludge (WAS), structural extracellular polymeric substances (St-EPS) resist WAS anaerobic fermentation. This study investigates the occurrence of polygalacturonate in WAS St-EPS by combining chemical and metagenomic analyses that identify ∼22% of the bacteria, including Ferruginibacter and...journal article 2023