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Filius, M. (author), Cui, T.J. (author), Ananth, A.N. (author), Docter, M.W. (author), Hegge, Jorrit W. (author), van der Oost, John (author), Joo, C. (author)Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of...journal article 2020
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Hegge, Jorrit W. (author), Swarts, Daan C. (author), Chandradoss, S.D. (author), Cui, T.J. (author), Kneppers, Jeroen (author), Jinek, Martin (author), Joo, C. (author), van der Oost, John (author)Prokaryotic Argonaute proteins (pAgos) constitute a diverse group of endonucleases of which some mediate host defense by utilizing small interfering DNA guides (siDNA) to cleave complementary invading DNA. This activity can be repurposed for programmable DNA cleavage. However, currently characterized DNA-cleaving pAgos require elevated...journal article 2019
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Cui, Thijs (author), Klein, M. (author), Hegge, Jorrit W. (author), Chandradoss, S.D. (author), van der Oost, John (author), Depken, S.M. (author), Joo, C. (author)Argonaute (Ago) proteins are key players in both gene regulation (eukaryotes) and host defense (prokaryotes). Acting on single-stranded nucleic-acid substrates, Ago relies on base pairing between a small nucleic-acid guide and its complementary target sequences for specificity. To efficiently scan nucleic-acid chains for targets, Ago diffuses...journal article 2019