- document
-
Boender, L.G.M. (author), De Hulster, E.A.F. (author), Van Maris, A.J.A. (author), Daran-Lapujade, P.A.S. (author), Pronk, J.T. (author)Growth at near-zero specific growth rates is a largely unexplored area of yeast physiology. To investigate the physiology of Saccharomyces cerevisiae under these conditions, the effluent removal pipe of anaerobic, glucose-limited chemostat culture (dilution rate, 0.025 h–1) was fitted with a 0.22-µm-pore-size polypropylene filter unit. This...journal article 2009
- document
-
Boender, L.G.M. (author), Van Maris, A.J.A. (author), De Hulster, E.A.F. (author), Almering, M.J.H. (author), Van der Klei, I.J. (author), Veenhuis, M. (author), De Winde, J.H. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)Extremely low specific growth rates (below 0.01 h?1) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is...journal article 2011
- document
-
Boender, L.G.M. (author), Almering, M.J.H. (author), Dijk, M. (author), Van Maris, A.J.A. (author), De Winde, J.H. (author), Pronk, J.T. (author), Daran-Lapujade, P. (author)Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and...journal article 2011