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Marques, W.L. (author), Mans, R. (author), Marella, Eko Roy (author), Cordeiro, Rosa Lorizolla (author), van den Broek, M.A. (author), Daran, J.G. (author), Pronk, J.T. (author), Gombert, Andreas K. (author), van Maris, A.J.A. (author)
Many relevant options to improve efficacy and kinetics of sucrose metabolism in Saccharomyces cerevisiae and, thereby, the economics of sucrose-based processes remain to be investigated. An essential first step is to identify all native sucrose-hydrolysing enzymes and sucrose transporters in this yeast, including those that can be activated...
journal article 2017
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Verhoeven, M.D. (author), Lee, Misun (author), Kamoen, L. (author), van den Broek, M.A. (author), Janssen, Dick B. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
Combined overexpression of xylulokinase, pentose-phosphate-pathway enzymes and a heterologous xylose isomerase (XI) is required but insufficient for anaerobic growth of Saccharomyces cerevisiae on d-xylose. Single-step Cas9-assisted implementation of these modifications yielded a yeast strain expressing Piromyces XI that showed fast aerobic...
journal article 2017
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van Rossum, H.M. (author), Kozak, B.U. (author), Niemeijer, M.S. (author), Dykstra, James C. (author), Luttik, M.A.H. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
In many eukaryotes, the carnitine shuttle plays a key role in intracellular transport of acyl moieties. Fatty acidgrown Saccharomyces cerevisiae cells employ this shuttle to translocate acetyl units into their mitochondria. Mechanistically, the carnitine shuttle should be reversible, but previous studies indicate that carnitine shuttle...
journal article 2016
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van Maris, A.J. (author), Luttik, M.A. (author), Winkler, A.A. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 2003
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Papapetridis, I. (author), Goudriaan, M. (author), Vazquez Vitali, M. (author), De Keijzer, Nikita A. (author), van den Broek, M.A. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
Background: Reduction or elimination of by-product formation is of immediate economic relevance in fermentation processes for industrial bioethanol production with the yeast Saccharomyces cerevisiae. Anaerobic cultures of wild-type S. cerevisiae require formation of glycerol to maintain the intracellular NADH/NAD<sup>+</sup> balance....
journal article 2018
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Vos, T. (author), Hakkaart, X.D.V. (author), de Hulster, A.F. (author), van Maris, A.J.A. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)
Background: Saccharomyces cerevisiae is an established microbial platform for production of native and non-native compounds. When product pathways compete with growth for precursors and energy, uncoupling of growth and product formation could increase product yields and decrease formation of biomass as a by-product. Studying non-growing,...
journal article 2016
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Bracher, J.M. (author), de Hulster, A.F. (author), Koster, C.C. (author), van den Broek, M.A. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
Biotin prototrophy is a rare, incompletely understood, and industrially relevant characteristic of Saccharomyces cerevisiae strains. The genome of the haploid laboratory strain CEN.PK113-7D contains a full complement of biotin biosynthesis genes, but its growth in biotin-free synthetic medium is extremely slow (specific growth rate [μ] ≈ 0.01...
journal article 2017
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van Maris, A.J. (author), Geertman, J.M. (author), Vermeulen, A. (author), Groothuizen, M.K. (author), Winkler, A.A. (author), Piper, M.D. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 2004
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Papapetridis, I. (author), van Dijk, M. (author), Dobbe, Arthur P A (author), Metz, B. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)
Background: Acetic acid, an inhibitor of sugar fermentation by yeast, is invariably present in lignocellulosic hydrolysates which are used or considered as feedstocks for yeast-based bioethanol production. Saccharomyces cerevisiae strains have been constructed, in which anaerobic reduction of acetic acid to ethanol replaces glycerol formation...
journal article 2016
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Mans, R. (author), Hassing, E. (author), Wijsman, M. (author), Giezekamp, Annabel (author), Pronk, J.T. (author), Daran, J.G. (author), van Maris, A.J.A. (author)
CRISPR/Cas9-based genome editing allows rapid, simultaneous modification of multiple genetic loci in Saccharomyces cerevisiae. Here, this technique was used in a functional analysis study aimed at identifying the hitherto unknown mechanism of lactate export in this yeast. First, an S. cerevisiae strain was constructed with deletions in 25...
journal article 2017
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Papapetridis, I. (author), Verhoeven, M.D. (author), Wiersma, S.J. (author), Goudriaan, M. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
Simultaneous fermentation of glucose and xylose can contribute to improved productivity and robustness of yeast-based processes for bioethanol production from lignocellulosic hydrolysates. This study explores a novel laboratory evolution strategy for identifying mutations that contribute to simultaneous utilisation of these sugars in batch...
journal article 2018
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Marques, W.L. (author), van der Woude, L.N. (author), Luttik, M.A.H. (author), van den Broek, M.A. (author), Nijenhuis, J.M. (author), Pronk, J.T. (author), van Maris, A.J.A. (author), Mans, R. (author), Gombert, Andreas K. (author)
Knowledge on the genetic factors important for the efficient expression of plant transporters in yeast is still very limited. Phaseolus vulgaris sucrose facilitator 1 (PvSuf1), a presumable uniporter, was an essential component in a previously published strategy aimed at increasing ATP yield in Saccharomyces cerevisiae. However, attempts to...
journal article 2018
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Verhoeven, M.D. (author), de Valk, S.C. (author), Daran, J.G. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
D-Glucose, D-xylose and L-arabinose are major sugars in lignocellulosic hydrolysates. This study explores fermentation of glucose-xylose-arabinose mixtures by a consortium of three ‘specialist’ Saccharomyces cerevisiae strains. A D-glucose- and L-arabinose-tolerant xylose specialist was constructed by eliminating hexose phosphorylation in an...
journal article 2018
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Marques, W.L. (author), Mans, R. (author), Henderson, Ryan K. (author), Marella, Eko Roy (author), ter Horst, J. (author), de Hulster, A.F. (author), Poolman, Bert (author), Daran, J.G. (author), Pronk, J.T. (author), Gombert, Andreas K. (author), van Maris, A.J.A. (author)
Anaerobic industrial fermentation processes do not require aeration and intensive mixing and the accompanying cost savings are beneficial for production of chemicals and fuels. However, the free-energy conservation of fermentative pathways is often insufficient for the production and export of the desired compounds and/or for cellular growth...
journal article 2018
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Jürgens, H. (author), Niemeijer, M.S. (author), Jennings-Antipov, Laura D. (author), Mans, R. (author), Morel, Jack (author), van Maris, A.J.A. (author), Pronk, J.T. (author), Gardner, Timothy S. (author)
Open data in science requires precise definition of experimental procedures used in data generation, but traditional practices for sharing protocols and data cannot provide the required data contextualization. Here, we explore implementation, in an academic research setting, of a novel cloud-based software system designed to address this...
journal article 2018
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van Maris, A.J. (author), Winkler, A.A. (author), Porro, D. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 2004
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Gonzalez Ramos, D. (author), Gorter de Vries, A.R. (author), Grijseels, Sietske S. (author), van Berkum, M.C. (author), Swinnen, Steve (author), van den Broek, M.A. (author), Nevoigt, Elke (author), Daran, J.G. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)
Background: Acetic acid, released during hydrolysis of lignocellulosic feedstocks for second generation bioethanol production, inhibits yeast growth and alcoholic fermentation. Yeast biomass generated in a propagation step that precedes ethanol production should therefore express a high and constitutive level of acetic acid tolerance before...
journal article 2016
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Papapetridis, I. (author), van Dijk, M. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
Background: Glycerol, whose formation contributes to cellular redox balancing and osmoregulation in Saccharomyces cerevisiae, is an important by-product of yeast-based bioethanol production. Replacing the glycerol pathway by an engineered pathway for NAD<sup>+</sup>-dependent acetate reduction has been shown to improve ethanol yields and...
journal article 2017
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Kozak, B.U. (author), Rossum, Harmen M. (author), Niemeijer, M.S. (author), van Dijk, M. (author), Benjamin, Kirsten (author), Wu, Liang (author), Daran, J.G. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)
In Saccharomyces cerevisiae ethanol dissimilation is initiated by its oxidation and activation to cytosolic acetyl-CoA. The associated consumption of ATP strongly limits yields of biomass and acetyl-CoA-derived products. Here, we explore the implementation of an ATP-independent pathway for acetyl-CoA synthesis from ethanol that, in theory,...
journal article 2016
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Jansen, Mickel L.A. (author), Bracher, J.M. (author), Papapetridis, I. (author), Verhoeven, M.D. (author), de Bruijn, J.A. (author), de Waal, P. (author), van Maris, A.J.A. (author), Klaassen, P (author), Pronk, J.T. (author)
The recent start-up of several full-scale ‘second generation’ ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. After a discussion of the challenges that these novel industrial contexts impose on yeast strains,...
journal article 2017
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