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van Loosdrecht, Mark C.M. (author), Hao, Xiaodi (author), Wu, Yuanyuan (author)摘要： 好氧颗粒污泥技术具有占地面积小、能耗低、剩余污泥量少、抗冲击性强等优点，是<br/>一种可持续的污水处理技术。以北京某污水处理厂实际生活污水作为基质，在中试规模（有效容积<br/>为2.5 m3）反应器中进行好氧颗粒污泥培养，研究了颗粒污泥在低温条件下的稳定性。中试自夏季<br/>开始运行到次年春季结束，共计运行260 d。结果表明，采用我国实际低碳源市政污水可以培养出好<br/>氧颗粒污泥，污泥颗粒化程度可达94%，SVI5/SVI30为1.1 ~ 1.3。冬季低温会造成颗粒污泥的枝状结<br/>构增多、丝状菌生长，进而引起颗粒化比例略有下降，为80%~91%，污泥平均粒径降低，SVI5/SVI30升<br/>高至1.5 ~ 1.8。在冬季低温条件下，通过优化调控进水流量...journal article 2022
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Kim, Nam Kyeun (author), Mao, Ningtao (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)In this research, extracellular polymeric substances (EPS), such as EPS<sub>flocs</sub> and EPS<sub>granules</sub>, were successfully extracted from activated and aerobic granular sludge, respectively, and tested as bio-based flame retardant materials. Flax fabric was coated by the biopolymeric substances and its flammability was evaluated...journal article 2020
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Haaksman, V.A. (author), Mirghorayshi, M. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Operational disturbances in aerobic granular sludge (AGS) systems can result in aerobic availability of readily biodegradable COD (rbCOD). Different from activated sludge, morphological consequences on the short and long term are not well described in literature. This study investigated the effect of incomplete anaerobic uptake of acetate on...journal article 2020
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Toja Ortega, S. (author), Pronk, M. (author), de Kreuk, M.K. (author)Complex substrates, like proteins, carbohydrates, and lipids, are major components of domestic wastewater, and yet their degradation in biofilm-based wastewater treatment technologies, such as aerobic granular sludge (AGS), is not well understood. Hydrolysis is considered the rate-limiting step in the bioconversion of complex substrates, and as...journal article 2021
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Felz, S. (author), Kleikamp, H.B.C. (author), Zlopasa, J. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Structural extracellular polymeric substances (structural EPS) can form stable hydrogels and are considered to be responsible for the stability of biofilms and aerobic granular sludge. Structural EPS were extracted from aerobic granular sludge and characterized for their gel-forming capacity with different alkaline earth and transition metal...journal article 2020
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Duque, Anouk F. (author), Bessa, Vânia S. (author), van Dongen, L.G.J.M. (author), de Kreuk, M.K. (author), Mesquita, Raquel B.R. (author), Rangel, António O.S.S. (author), van Loosdrecht, Mark C.M. (author), Castro, Paula M.L. (author)The presence of toxic compounds in wastewater can cause problems for organic matter and nutrient removal. In this study, the long-term effect of a model xenobiotic, 2-fluorophenol (2-FP), on ammonia-oxidizing bacteria (AOB), nitrite oxidizing bacteria (NOB) and phosphate accumulating organisms (PAO) in aerobic granular sludge was investigated...journal article 2021
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Toja Ortega, S. (author), Pronk, M. (author), de Kreuk, M.K. (author)High concentrations of particulate COD (pCOD) in the influent of aerobic granular sludge (AGS) systems are often associated to small granule diameter and a large fraction of flocculent sludge. At high particulate concentrations even granule stability and process performance might be compromised. However, pilot-or full-scale studies focusing...journal article 2021
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van Dijk, E.J.H. (author), Haaksman, V.A. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)In this study a mathematical framework was developed to describe aerobic granulation based on 6 main mechanisms: microbial selection, selective wasting, maximizing transport of substrate into the biofilm, selective feeding, substrate type and breakage. A numerical model was developed using four main components; a 1D convection/dispersion...journal article 2022
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Toja Ortega, S. (author), van den Berg, L. (author), Pronk, M. (author), de Kreuk, M.K. (author)In aerobic granular sludge (AGS) reactors, granules of different sizes coexist in a single reactor. Their differences in settling behaviour cause stratification in the settled granule bed. In combination with substrate concentration gradients over the reactor height during the anaerobic plug-flow feeding regime, this can result in functional...journal article 2022
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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Cingoz, Seyma (author), Yoruk, Mustafa (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author), Ersahin, Mustafa Evren (author)Achieving a neutral/positive energy balance without compromising discharge standards is one of the main goals of wastewater treatment plants (WWTPs) in terms of sustainability. Aerobic granular sludge (AGS) technology promises high treatment performance with low energy and footprint requirement. In this study, high-rate activated sludge (HRAS...journal article 2023
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Trebuch, Lukas M. (author), Sohier, Jasper (author), Altenburg, Sido (author), Oyserman, Ben O. (author), Pronk, M. (author), Janssen, Marcel (author), Vet, Louise E.M. (author), Wijffels, René H. (author), Fernandes, Tânia V. (author)Photogranules are a novel wastewater treatment technology that can utilize the sun's energy to treat water with lower energy input and have great potential for nutrient recovery applications. They have been proven to efficiently remove nitrogen and carbon but show lower conversion rates for phosphorus compared to established treatment systems...journal article 2023
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van Dijk, E.J.H. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)The nitrous oxides emission was measured over 7 months in the full-scale aerobic granular sludge plant in Dinxperlo, the Netherlands. Nitrous oxide concentrations were measured in the bulk liquid and the off-gas of the Nereda® reactor. Combined with the batch wise operation of the reactor, this gave a high information density and a better...journal article 2021
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Felz, S. (author), Al-Zuhairy, S.H.K. (author), Aarstad, Olav Andreas (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)To evaluate and develop methodologies for the extraction of gel-forming extracellular polymeric substances (EPS), EPS from aerobic granular sludge (AGS) was extracted using six different methods (centrifugation, sonication, ethylenediaminetetraacetic acid (EDTA), formamide with sodium hydroxide (NaOH), formaldehyde with NaOH and sodium...journal article 2016
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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Sagir Kurt, Ece (author), Atli, Ezgi (author), Basa, Safak (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author)Aerobic granules contain microorganisms that are responsible for carbon, nitrogen, and phosphorus removal in aerobic granular sludge (AGS) process in which aerobic/anoxic/anaerobic layers (from surface to core) occur in a single granule. Optimizing the aerobic granular sludge (AGS) process for granulation and efficient nutrient removal can be...journal article 2022
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Pronk, M. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 °C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid...journal article 2017
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de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Many sources of wastewater contain sulfides, which can cause excessive growth of filamentous bacteria such as Thiothrix sp. resulting in bulking sludge in conventional activated sludge systems. Granular sludge systems could potentially also suffer from the growth of filamentous bacteria. Uptake of easily degradable COD by the relatively slow...journal article 2020
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Barrios Hernandez, M.L. (author), Buenaño-Vargas, Claribel (author), Garcia, H. (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Hooijmans, Christine M. (author)The co-treatment of two synthetic faecal sludges (FS-1 and FS-2) with municipal synthetic wastewater (WW) was evaluated in an aerobic granular sludge (AGS) reactor. After characterisation, FS-1 showed the following concentrations, representative for medium-strength FS: 12,180 mg TSS L<sup>−1</sup>, 24,300 mg total COD L<sup>−1</sup>, 93.8 mg...journal article 2020
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Barrios Hernandez, M.L. (author), Pronk, M. (author), Garcia, H. (author), Boersma, Arne (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Hooijmans, Christine M. (author)The aim of this study was to evaluate the effectiveness of the novel aerobic granular sludge (AGS) wastewater treatment technology in removing faecal indicator organisms (FIOs) compared to the conventional activated sludge (CAS) treatment system. The work was carried out at two full-scale wastewater treatment plants (WWTP) in the Netherlands,...journal article 2020
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Felz, S. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Glycosaminoglycans (GAGs) are linear heteropolysaccharides containing a derivative of an amino sugar. The possibility of the presence of GAGs in aerobic granular sludge was studied by combining SDS-PAGE with Alcian Blue staining (at pH 2.5 and 1), FTIR, mammalian Hyaluronic acid and sulfated GAG analysis kits, enzymatic digestion and specific...journal article 2020
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de Graaff, D.R. (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Seawater can be introduced or intrude in sewer systems and can thereby negatively influence biological wastewater treatment processes. Here we studied the impact of artificial seawater on the enhanced biological phosphate removal (EBPR) process performance by aerobic granular sludge (AGS) with synthetic wastewater. Process performance,...journal article 2020
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