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de Graaff, D.R. (author), van Loosdrecht, M.C.M. (author), Pronk, M. (author)
Seawater can be introduced or intrude in sewer systems and can thereby negatively influence biological wastewater treatment processes. Here we studied the impact of artificial seawater on the enhanced biological phosphate removal (EBPR) process performance by aerobic granular sludge (AGS) with synthetic wastewater. Process performance,...
journal article 2020
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Feng, Cuijie (author), Welles, L. (author), Zhang, X. (author), Pronk, M. (author), de Graaff, D.R. (author), van Loosdrecht, M.C.M. (author)
Phosphorus has been successfully eliminated from wastewater by biological techniques of enhanced biological phosphorus removal (EBPR) process, which relies on a specific microbiota of polyphosphate accumulating organisms (PAOs) that accumulate phosphate as polyphosphates (poly-P). Most methods for quantification of poly-P pools suffer from...
journal article 2020
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Barrios Hernandez, M.L. (author), Pronk, M. (author), Garcia, H. (author), Boersma, Arne (author), Brdanovic, D. (author), van Loosdrecht, M.C.M. (author), Hooijmans, Christine M. (author)
The aim of this study was to evaluate the effectiveness of the novel aerobic granular sludge (AGS) wastewater treatment technology in removing faecal indicator organisms (FIOs) compared to the conventional activated sludge (CAS) treatment system. The work was carried out at two full-scale wastewater treatment plants (WWTP) in the Netherlands,...
journal article 2020
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Valk, L.C. (author), Diender, Martijn (author), Stouten, G.R. (author), Petersen, Jette F. (author), Nielsen, Per H. (author), Dueholm, Morten S. (author), Pronk, J.T. (author), van Loosdrecht, M.C.M. (author)
Acetogens have the ability to fixate carbon during fermentation by employing the Wood-Ljungdahl pathway (WLP), which is highly conserved across Bacteria and Archaea. In a previous study, product stoichometries in galacturonate-limited, anaerobic enrichment cultures of “Candidatus Galacturonibacter soehngenii,” from a novel genus within the...
journal article 2020
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Perli, T. (author), Wronska, A.K. (author), Ortiz Merino, R.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Chemically defined media for yeast cultivation (CDMY) were developed to support fast growth, experimental reproducibility, and quantitative analysis of growth rates and biomass yields. In addition to mineral salts and a carbon substrate, popular CDMYs contain seven to nine B-group vitamins, which are either enzyme cofactors or precursors for...
journal article 2020
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Valk, L.C. (author), Luttik, M.A.H. (author), de Ram, C. (author), Pabst, M. (author), van den Broek, M.A. (author), van Loosdrecht, M.C.M. (author), Pronk, J.T. (author)
D-galacturonate, a key constituent of pectin, is a ubiquitous monomer in plant biomass. Anaerobic, fermentative conversion of D-galacturonate is therefore relevant in natural environments as well as in microbial processes for microbial conversion of pectin-containing agricultural residues. In currently known microorganisms that anaerobically...
journal article 2020
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van der Meij, Leander (author), Demetriou, A.M. (author), Tulin, Marina (author), Méndez, Ileana (author), Dekker, Peter (author), Pronk, Tila (author)
There is evidence that testosterone and cortisol levels are related to the attraction of a romantic partner; testosterone levels relate to a wide range of sexual behaviors and cortisol is a crucial component in the response to stress. To investigate this, we conducted a speed-dating study among heterosexual singles. We measured salivary...
journal article 2019
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Liu, Y. (author), El Masoudi, Anissa (author), Pronk, J.T. (author), van Gulik, W.M. (author)
So far, the physiology of Saccharomyces cerevisiae at near-zero growth rates has been studied in retentostat cultures with a growth-limiting supply of the carbon and energy source. Despite its relevance in nature and industry, the near-zero growth physiology of S. cerevisiae under conditions where growth is limited by the supply of non-energy...
journal article 2019
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Hassing, E. (author), de Groot, P.A. (author), Marquenie, Vita R. (author), Pronk, J.T. (author), Daran, J.G. (author)
The organic compound 2-phenylethanol (2PE) has a pleasant floral scent and is intensively used in the cosmetic and food industries. Microbial production of 2PE by phenylalanine bioconversion or de novo biosynthesis from sugar offer sustainable, reliable and natural production processes compared to chemical synthesis. Despite the ability of...
journal article 2019
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Gorter de Vries, A.R. (author), Koster, C.C. (author), Weening, S.M. (author), Luttik, M.A.H. (author), Kuijpers, N.G.A. (author), Geertman, Jan Maarten A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Interspecies hybrids of Saccharomyces species are found in a variety of industrial environments and often outperform their parental strains in industrial fermentation processes. Interspecies hybridization is therefore increasingly considered as an approach for improvement and diversification of yeast strains for industrial application....
journal article 2019
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de Graaff, D.R. (author), Felz, S. (author), Neu, Thomas R. (author), Pronk, M. (author), van Loosdrecht, M.C.M. (author), Lin, Y. (author)
Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by...
journal article 2019
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Gorter de Vries, A.R. (author), Pronk, J.T. (author), Daran, J.G. (author)
The yeast Saccharomyces pastorianus is responsible for the annual worldwide production of almost 200 billion liters of lager-type beer. S. pastorianus is a hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus that has been studied for well over a century. Scientific interest in S. pastorianus intensified upon the discovery, in 2011,...
journal article 2019
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Baldi, N. (author), Dykstra, James C. (author), Luttik, M.A.H. (author), Pabst, M. (author), Wu, Liang (author), Benjamin, Kirsten R. (author), Vente, André (author), Pronk, J.T. (author), Mans, R. (author)
Efficient production of fuels and chemicals by metabolically engineered micro-organisms requires availability of precursor molecules for product pathways. In eukaryotic cell factories, heterologous product pathways are usually expressed in the cytosol, which may limit availability of precursors that are generated in other cellular...
journal article 2019
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Gorter de Vries, A.R. (author), Voskamp, Maaike (author), van Aalst, Aafke (author), Kristensen, L.H. (author), Jansen, Liset (author), Salazar, A.N. (author), van den Broek, M.A. (author), Brouwers, N. (author), Abeel, T.E.P.M.F. (author), Pronk, J.T. (author), Daran, J.G. (author)
Saccharomyces pastorianus lager-brewing yeasts are domesticated hybrids of S. cerevisiae x S. eubayanus that display extensive inter-strain chromosome copy number variation and chromosomal recombinations. It is unclear to what extent such genome rearrangements are intrinsic to the domestication of hybrid brewing yeasts and whether they...
journal article 2019
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Dekker, W.J.C. (author), Wiersma, S.J. (author), Bouwknegt, J. (author), Mooiman, C. (author), Pronk, J.T. (author)
In Saccharomyces cerevisiae, acyl-coenzyme A desaturation by Ole1 requires molecular oxygen. Tween 80, a poly-ethoxylated sorbitan-oleate ester, is therefore routinely included in anaerobic growth media as a source of unsaturated fatty acids (UFAs). During optimization of protocols for anaerobic bioreactor cultivation of this yeast, we...
journal article 2019
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Hakkaart, X.D.V. (author), Liu, Y. (author), Hulst, Mandy (author), el Masoudi, Anissa (author), Peuscher, Eveline (author), Pronk, J.T. (author), van Gulik, W.M. (author), Daran-Lapujade, P.A.S. (author)
Engineered strains of Saccharomyces cerevisiae are used for industrial production of succinic acid. Optimal process conditions for dicarboxylic-acid yield and recovery include slow growth, low pH, and high CO<sub>2</sub>. To quantify and understand how these process parameters affect yeast physiology, this study investigates individual and...
journal article 2019
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Venturini, T. (author), Turrin, M. (author), Setaki, F. (author), Veer, F.A. (author), Pronk, Arno (author), Teuffel, Patrick (author), Moonen, Yaron (author), Slangen, Stefan (author), Vorstermans, Rens (author)
In recent years, natural disaster and military conflicts forced vast numbers of people to flee their home countries, contributing to the migration crisis we are facing today. According to the UNHCR, the number of forcibly displaced people worldwide reached the highest level since World War II. Post-disaster housing is by nature diverse and...
journal article 2019
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Brouwers, N. (author), Gorter de Vries, A.R. (author), van den Broek, M.A. (author), Weening, S.M. (author), Elink Schuurman, Tom D. (author), Kuijpers, Niels G.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Saccharomyces eubayanus is the non-S. cerevisiae parent of the lager-brewing hybrid S. pastorianus. In contrast to most S. cerevisiae and Frohberg-type S. pastorianus strains, S. eubayanus cannot utilize the α-tri-glucoside maltotriose, a major carbohydrate in brewer's wort. In Saccharomyces yeasts, utilization of maltotriose is encoded by...
journal article 2019
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Papapetridis, I. (author), Goudriaan, M. (author), Vazquez Vitali, M. (author), De Keijzer, Nikita A. (author), van den Broek, M.A. (author), van Maris, A.J.A. (author), Pronk, J.T. (author)
Background: Reduction or elimination of by-product formation is of immediate economic relevance in fermentation processes for industrial bioethanol production with the yeast Saccharomyces cerevisiae. Anaerobic cultures of wild-type S. cerevisiae require formation of glycerol to maintain the intracellular NADH/NAD<sup>+</sup> balance....
journal article 2018
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Jürgens, H. (author), Varela, Javier A. (author), Gorter de Vries, A.R. (author), Perli, T. (author), Gast, V.J.M. (author), Gyurchev, N.Y. (author), Rajkumar, Arun S. (author), Mans, R. (author), Pronk, J.T. (author), Morrissey, John P. (author), Daran, J.G. (author)
While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR tools that function in multiple yeast species could contribute to the intensifying research on non-conventional yeasts. A plasmid carrying a pangenomic origin of replication...
journal article 2018
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