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Haridas, M. (author), Bisterfeld, C. (author), Chen, Le Min (author), Marsden, S.R. (author), Tonin, F. (author), Medici, R. (author), Iribarren, Adolfo (author), Lewkowicz, Elizabeth (author), Hagedoorn, P.L. (author), Hanefeld, U. (author), Abdelraheem, E.M.M. (author)
DERA (2-Deoxy-D-ribose 5-phosphate aldolase) is the only known aldolase that accepts two aldehyde substrates, which makes it an attractive catalyst for the synthesis of a chiral polyol motif that is present in several pharmaceuticals, such as atorvastatin and pravastatin. However, inactivation of the enzyme in the presence of aldehydes...
journal article 2020
document
Chen, B. (author), Medici, R. (author), van der Helm, M. (author), van Zwet, Y.A.A. (author), Gjonaj, L. (author), van der Geest, R.C.S. (author), Otten, L.G. (author), Hanefeld, U. (author)
Rhodococcus strains are ubiquitous in nature and known to metabolise a wide variety of compounds. At the same time, asymmetric reduction of C=C bonds is important in the production of high-valued chiral building blocks. In order to evaluate if Rhodococci can be used for this task, we have probed several Rhodococcus rhodochrous and R....
journal article 2018
document
Chen, B.S. (author), Resch, V. (author), Otten, L.G. (author), Hanefeld, U. (author)
The enantioselective Michael addition using water as both nucleophile and solvent has to date proved beyond the ability of synthetic chemists. Herein, the direct, enantioselective Michael addition of water in water to prepare important ?-hydroxy carbonyl compounds using whole cells of Rhodococcus strains is described. Good yields and excellent...
journal article 2014
document
Resch, V.A. (author), Jin, J. (author), Chen, B.S. (author), Hanefeld, U. (author)
The Michael hydratase – alcohol dehydrogenase (MhyADH) from Alicycliphilus denitrificans was previously identified as a bi-functional enzyme performing a hydration of ?,?-unsaturated ketones and subsequent oxidation of the formed alcohols. The investigations of the bi-functionality were based on a spectrophotometric assay and an activity...
journal article 2014
document
Resch, V. (author), Seidler, C. (author), Chen, B.S. (author), Degeling, I. (author), Hanefeld, U. (author)
The use of water as a nucleophile for Michael additions is still a challenge in organic chemistry. In this report we describe the use of amino acids as catalysts for the Michael addition of water to ?,?-unsaturated ketones. All 20 proteinogenic amino acids were screened and L-lysine was identified as the best candidate. To obtain a better...
journal article 2013
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Chen, B.S. (author), Otten, L.G. (author), Resch, V. (author), Muyzer, G. (author), Hanefeld, U. (author)
Rhodococcus rhodochrous ATCC 17895 possesses an array of mono- and dioxygenases, as well as hydratases, which makes it an interesting organism for biocatalysis. R. rhodochrous is a Gram-positive aerobic bacterium with a rod-like morphology. Here we describe the features of this organism, together with the complete genome sequence and annotation....
journal article 2013
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