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Coloma Hurel, J.L. (author), Hagedoorn, P.L. (author), Bento, Isabel (author), Hanefeld, U. (author)
Granulicella tundricola hydroxynitrile lyase (GtHNL) is a manganese dependent cupin that catalyzes the enantioselective synthesis of cyanohydrins. The analysis of its active site shows high similarity with the active site of cupin Tm1459 from Thermotoga maritima, an enzyme that catalyzes the oxidative cleavage of styrene derivatives. GtHNL ...
journal article 2023
document
Bracco Garcia, M.P. (author), Torrelo Villa, G. (author), Noordam, Sander (author), de Jong, Glenn (author), Hanefeld, U. (author)
The hydroxynitrile lyase from Prunus amygdalus was immobilized on Celite R-633. The immobilized enzyme could successfully be utilized in buffer saturated MTBE and excellent conversions of benzaldehyde to R-mandelonitrile were observed. No leaching occurred. To achieve high enantioselectivities, the suppression of the undesired background...
journal article 2018