Precision Engineering of the Co-immobilization of Enzymes for Cascade Biocatalysis

The design and orderly layered co-immobilization of multiple enzymes on resin particles remain challenging. In this study, the SpyTag/SpyCatcher binding pair was fused to the N-terminus of an alcohol dehydrogenase (ADH) and an aldo-keto reductase (AKR), respectively. A non-canonical amino acid (ncAA), p-azido-L-phenylalanine (p-AzF), as the...

Designing an enzyme assembly line for green cascade processes using bio-orthogonal chemistry

Two non-canonical amino acids (ncAAs) with bio-orthogonal reactive groups, namely, p-azido-l-phenylalanine (p-AzF) and p-propargyloxy-l-phenylalanine (p-PaF), were genetically inserted into an aldo-keto reductase (AKR) and an alcohol dehydrogenase (ADH), respectively, at two preselected sites for each enzyme. The variants were expressed in...

A Fixed-Wing UAV Formation Algorithm Based on Vector Field Guidance

The vector field method was originally proposed to guide a single fixed-wing Unmanned Aerial Vehicle (UAV) towards a desired path. In this work, a non-uniform vector field method is proposed that changes in both magnitude and direction, for the purpose of achieving formations of UAVs. As compared to related work in the literature, the...

A multi-axis robot-based bioprinting system supporting natural cell function preservation and cardiac tissue fabrication

Despite the recent advances in artificial tissue and organ engineering, how to generate large size viable and functional complex organs still remains as a grand challenge for regenerative medicine. Three-dimensional bioprinting has demonstrated its advantages as one of the major methods in fabricating simple tissues, yet it still faces...

Local human activities overwhelm decreased sediment supply from the Changjiang River: Continued rapid accumulation in the Hangzhou Bay-Qiantang Estuary system

We investigate the morphological responses of the Hangzhou Bay, China, located immediately south of the Changjiang Estuary, to the drastic reduction of the sediment load from the Changjiang River and the large-scale coastal embankment schemes over past decades. The spatial patterns of deposition and erosion, sediment volume changes, and the...

Morphodynamic modeling of a large inside sand bar and its dextral morphology in a convergent estuary: Qiantang Estuary, China

We investigate the evolution of a large-scale sand body, a unique type of sandbars in a convergent estuary. Specifically, we analyze and simulate the sand deposition system (defined as an inside bar) in the Qiantang Estuary (QE) in China. The deposit is 130 km long and up to 10 m thick, and is characterized by a dextral morphology in the lower...

The variations of sediment transport patterns in the outer Changjiang Estuary and Hangzhou Bay over the last 30 years

The research objective is to investigate the variations of sediment transport in outer Changjiang Estuary and adjacent Hangzhou Bay, induced by the decline of Changjiang River sediment discharge and massive land reclamation in the last three decades. A synchronous hydrographic survey was conducted along two transects (at the bay-mouth and outer...