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De Jonge, L.P. (author), Douma, R.D. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author)
A sampling procedure for quantitative metabolomics in Penicillium chrysogenum based on cold aqueous methanol quenching was re-evaluated and optimized to reduce metabolite leakage during sample treatment. The optimization study included amino acids and intermediates of the glycolysis and the TCA-cycle. Metabolite leakage was found to be minimal...
journal article 2011
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Zhao, Z. (author), Ten Pierick, A. (author), De Jonge, L. (author), Heijnen, J.J. (author), Wahl, S.A. (author)
Background Penicillium chrysogenum, the main production strain for penicillin-G, has a high content of intracellular carbohydrates, especially reduced sugars such as mannitol, arabitol, erythritol, as well as trehalose and glycogen. In previous steady state 13C wash-in experiments a delay of labeling enrichments in glycolytic intermediates was...
journal article 2012