Searched for:
(1 - 8 of 8)
document
Dulin, D. (author), Vilfan, I.D. (author), Berghuis, B.A. (author), Hage, S. (author), Bamford, D.H. (author), Poranen, M.M. (author), Depken, S.M. (author), Dekker, N.H. (author)
RNA viruses have specific mutation rates that balance the conflicting needs of an evolutionary response to host antiviral defenses and avoidance of the error catastrophe. While most mutations are known to originate in replication errors, difficulties of capturing the underlying dynamics have left the mechanochemical basis of viral mutagenesis...
journal article 2015
document
De Vlaminck, I. (author), Van Loenhout, M.T.J. (author), Zweifel, L. (author), Den Blanken, J. (author), Hooning, K. (author), Hage, S. (author), Kerssemakers, J. (author), Dekker, C. (author)
In E. coli homologous recombination, a filament of RecA protein formed on DNA searches and pairs a homologous sequence within a second DNA molecule with remarkable speed and fidelity. Here, we directly probe the strength of the two-molecule interactions involved in homology search and recognition using dual-molecule manipulation, combining...
journal article 2012
document
Vilfan, I.D. (author), Candelli, A. (author), Hage, S. (author), Aalto, A.P. (author), Poranen, M.M. (author), Bamford, D.H. (author), Dekker, N.H. (author)
RNA-dependent RNA polymerases (RdRP) form an important class of enzymes that is responsible for genome replication and transcription in RNA viruses and involved in the regulation of RNA interference in plants and fungi. The RdRP kinetics have been extensively studied, but pausing, an important regulatory mechanism for RNA polymerases that has...
journal article 2008
document
Crut, A. (author), Koster, D.A. (author), Huang, Z. (author), Hage, S. (author), Dekker, N.H. (author)
journal article 2008
document
Vilfan, I.D. (author), Candelli, A. (author), Hage, S. (author), Aalto, A.P. (author), Poranen, M.M. (author), Bamford, D.H. (author), Dekker, N.H. (author)
RNA-dependent RNA polymerases (RdRP) form an important class of enzymes that is responsible for genome replication and transcription in RNA viruses and involved in the regulation of RNA interference in plants and fungi. The RdRP kinetics have been extensively studied, but pausing, an important regulatory mechanism for RNA polymerases that has...
journal article 2008
document
Van den Hout, M. (author), Hage, S. (author), Dekker, C. (author), Dekker, N.H. (author)
Many experiments involving nucleic acids require the hybridization and ligation of multiple DNA or RNA molecules to form a compound molecule. When one of the constituents is single stranded, however, the efficiency of ligation can be very low and requires significant individually tailored optimization. Also, when the molecules involved are very...
journal article 2008
document
Vilfan, I.D. (author), Kamping, W. (author), Van den Hout, M. (author), Candelli, A. (author), Hage, S. (author), Dekker, N.H. (author)
Precise, controllable single-molecule force spectroscopy studies of RNA and RNA-dependent processes have recently shed new light on the dynamics and pathways of RNA folding and RNAenzyme interactions. A crucial component of this research is the design and assembly of an appropriate RNA construct. Such a construct is typically subject to several...
journal article 2007
document
Besteman, K. (author), Hage, S. (author), Dekker, N.H. (author), Lemay, G. (author)
journal article 2007
Searched for:
(1 - 8 of 8)