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Lee, M. (author), Lipfert, J. (author), Sanchez, H. (author), Wyman, C. (author), Dekker, N.H. (author)
Human RAD51 is a key protein in the repair of DNA by homologous recombination. Its assembly onto DNA, which induces changes in DNA structure, results in the formation of a nucleoprotein filament that forms the basis of strand exchange. Here, we determine the structural and mechanical properties of RAD51-dsDNA filaments. Our measurements use two...
journal article 2013
document
Holthausen, J.T. (author), Van Loenhout, M.T.J. (author), Sanchez, H. (author), Ristic, D. (author), Van Rossum-Fikkert, S.E. (author), Modesti, M. (author), Dekker, C. (author), Kanaar, R. (author), Wyman, C. (author)
Homologous recombination is essential for the preservation of genome stability, thereby preventing cancer. The recombination protein RAD51 drives DNA strand exchange, which requires the assembly, rearrangement and disassembly of a RAD51 filament on DNA, coupled to ATP binding and hydrolysis. This process is facilitated and controlled by...
journal article 2011
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Van Loenhout, M.T.J. (author), Van der Heijden, T. (author), Kanaar, R. (author), Wyman, C. (author), Dekker, C. (author)
RecA, the key protein in homologous recombination, performs its actions as a helical filament on single-stranded DNA (ssDNA). ATP hydrolysis makes the RecA–ssDNA filament dynamic and is essential for successful recombination. RecA has been studied extensively by single-molecule techniques on double-stranded DNA (dsDNA). Here we directly probe...
journal article 2009
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Van der Heijden, T. (author), Seidel, R. (author), Modesti, M. (author), Kanaar, R. (author), Wyman, C. (author), Dekker, C. (author)
The human DNA repair protein RAD51 is the crucial component of helical nucleoprotein filaments that drive homologous recombination. The molecular mechanistic details of how this structure facilitates the requisite DNA strand rearrangements are not known but must involve dynamic interactions between RAD51 and DNA. Here, we report the real-time...
journal article 2007
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van der Heijden, T. (author), van Noort, J. (author), van Leest, H. (author), Kanaar, R. (author), Wyman, C. (author), Dekker, N.H. (author), Dekker, N. (author), Dekker, C. (author)
journal article 2005
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van Noort, J. (author), van der Heijden, T. (author), de Jager, M. (author), Wyman, C. (author), Kanaar, R. (author), Dekker, C. (author)
journal article 2003
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Ristic, D. (author), Modesti, M. (author), Van der Heijden, T. (author), Van Noort, J. (author), Dekker, C. (author), Kanaar, R. (author), Wyman, C. (author)
Recombinase proteins assembled into helical filaments on DNA are believed to be the catalytic core of homologous recombination. The assembly, disassembly and dynamic rearrangements of this structure must drive the DNA strand exchange reactions of homologous recombination. The sensitivity of eukaryotic recombinase activity to reaction conditions...
journal article
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