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Hülsen, Tim (author), Venturato, Daniel (author), Chan, Clement (author), Vandi, Luigi (author), Laycock, Bronwyn (author), Pratt, Steven (author), Stegman, Samuel (author), van Loosdrecht, Mark C.M. (author), Batstone, Damien J. (author)Anoxygenic purple phototrophic bacteria (PPB) were utilised in an 80 L biofilm flat plate photobioreactor to generate polyhydroxyalkanoate (PHA) over 44 cycles, with acetate as feed. Over the cycles, net PHA yield (growth + accumulation) averaged 21% while accumulation yield averaged 55%. Average PHA content was 35 wt% volatile solids (VS),...journal article 2024
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Ahmadi, Navid (author), Abbasi, Mona (author), Torabian, Ali (author), van Loosdrecht, Mark C.M. (author), Ducoste, Joel (author)We investigated the transformation of four pharmaceuticals (Diclofenac, Naproxen, Ibuprofen and Carbamazepine) in a moving bed biofilm reactor subjected to different COD/N ratios in four experimental phases. The shift from medium to high range COD/N ratio (i.e., 5:1 to 100:1) intensified the competition between heterotrophs and nitrifying...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Desmond, Peter (author), Huisman, Kees Theo (author), Sanawar, Huma (author), Farhat, Nadia M. (author), Traber, Jacqueline (author), Fridjonsson, Einar O. (author), Johns, Michael L. (author), Flemming, Hans Curt (author), Picioreanu, C. (author), Vrouwenvelder, J.S. (author)The application of membrane technology for water treatment and reuse is hampered by the development of a microbial biofilm. Biofilm growth in micro-and ultrafiltration (MF/UF) membrane modules, on both the membrane surface and feed spacer, can form a secondary membrane and exert resistance to permeation and crossflow, increasing energy demand...journal article 2022
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Pirzadian, J. (author), Souhoka, T. (author), Herweijer, M. (author), van Heel, M.E. (author), van Wamel, W. J.B. (author), Goossens, R.H.M. (author), Severin, J. A. (author), Vos, M. C. (author)In hospitals, sinks act as reservoirs for bacterial pathogens. To assess the extent of splashing, fluorescein dye was added to four hospital sinks previously involved in pathogen dispersal to the environment and/or transmission to patients, and one sink that was not. Applying dye to the p-trap or tailpiece did not result in any fluorescent...journal article 2022
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Li, Jinmei (author), Zhang, Shuting (author), Guo, Lizheng (author), Chen, L. (author), Yu, Zhisheng (author)The generation and dissemination of antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs) in the environment has become a critical risk to human health. This study is based on a pilot-scale simulated water distribution system to understand the effects of chlorine disinfection treatment (without free chlorine) on ARB and...journal article 2021
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Kouijzer, Joop J.P. (author), Lattwein, Kirby R. (author), Beekers, Inés (author), Langeveld, Simone A.G. (author), Leon-Grooters, Mariël (author), Strub, Jean Marc (author), Oliva, Estefania (author), de Jong, N. (author), van der Steen, A.F.W. (author)Bacterial biofilms are a huge burden on our healthcare systems worldwide. The lack of specificity in diagnostic and treatment possibilities result in difficult-to-treat and persistent infections. The aim of this in vitro study was to investigate if microbubbles targeted specifically to bacteria in biofilms could be used both for diagnosis as...journal article 2021
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Ahmad, J.I. (author), Dignum, Marco (author), Liu, G. (author), Medema, G.J. (author), van der Hoek, J.P. (author)Drinking water distribution systems (DWDSs) have been thoroughly studied, but the concept of thermal energy recovery from DWDSs is very new and has been conceptualized in the past few years. Cold recovery results in a temperature increase of the drinking water. Its effects on drinking water quality and biofilm development are unclear. Hence, we...journal article 2021
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Pfaff, N.M. (author), Kleijn, J. Mieke (author), van Loosdrecht, Mark C.M. (author), Kemperman, Antoine J.B. (author)The properties of biofilm EPS are determined by the multiple interactions between its constituents and the surrounding environment. Because of the high complexity of biofilm EPS, its constituents' characterisation is still far from thorough, and identification of these interactions cannot be done yet. Therefore, we use gels of bacterial...journal article 2021
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Shi, Haoran (author)Drinking water safety is of great concern all around the world. Two strategies can be pursued to maintain biological stable drinking water during distribution in the network: (i) without a residual disinfectant, in which bacterial growth is controlled by very low biodegradable nutrients in the water by extensive pre-treatment and a well...master thesis 2020
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Felz, S. (author), Kleikamp, H.B.C. (author), Zlopasa, J. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Structural extracellular polymeric substances (structural EPS) can form stable hydrogels and are considered to be responsible for the stability of biofilms and aerobic granular sludge. Structural EPS were extracted from aerobic granular sludge and characterized for their gel-forming capacity with different alkaline earth and transition metal...journal article 2020
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van Dijk, B. (author), Lemans, J. V.C. (author), Hoogendoorn, R. M. (author), Dadachova, E. (author), de Klerk, J. M.H. (author), Vogely, H. C. (author), Weinans, Harrie (author), Lam, M. G.E.H. (author), van der Wal, B. C.H. (author)BACKGROUND: Widespread use and misuse of antibiotics have led to a dramatic increase in the emergence of antibiotic resistant bacteria, while the discovery and development of new antibiotics is declining. This has made certain implant-associated infections such as periprosthetic joint infections, where a biofilm is formed, very difficult to...review 2020
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Soliman, Mona Youssef Moawad (author), van Halem, D. (author), Medema, G.J. (author)Ceramic pot filters are household water treatment and safe storage (HWTS) systems designed to improve the microbial quality of drinking water. They yield high log reduction values (LRVs) for bacterial and protozoan pathogens but provide very little removal of viruses. This study investigated virus removal of ceramic filter discs (CFDs), using...journal article 2020
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Felz, S. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Glycosaminoglycans (GAGs) are linear heteropolysaccharides containing a derivative of an amino sugar. The possibility of the presence of GAGs in aerobic granular sludge was studied by combining SDS-PAGE with Alcian Blue staining (at pH 2.5 and 1), FTIR, mammalian Hyaluronic acid and sulfated GAG analysis kits, enzymatic digestion and specific...journal article 2020
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Ahmad, J.I. (author), Liu, G. (author), van der Wielen, Paul (author), Medema, G.J. (author), van der Hoek, J.P. (author)Drinking water distribution systems (DWDSs) are used to supply hygienically safe and biologically stable water for human consumption. The potential of thermal energy recovery from drinking water has been explored recently to provide cooling for buildings. Yet, the effects of increased water temperature induced by this “cold recovery” on the...journal article 2020
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Pinel, I.S.M. (author), Moed, D. H. (author), Vrouwenvelder, J.S. (author), van Loosdrecht, Mark C.M. (author)Understanding the bacterial dynamics in cooling towers is imperative for the assessment of disinfection efficiency and management of microbial risks linked to aerosol formation. The objective of this study was to evaluate the impact of feed water on the cooling water bacterial microbiome and investigate the survival ability of its members...journal article 2020
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Kirkland, Catherine M. (author), Seymour, Joseph D. (author), Krug, Julia R. (author), Velders, Aldrik H. (author), Vergeldt, Frank J. (author), Van As, Henk (author), van den Berg, L. (author), de Kreuk, M.K. (author), Codd, Sarah L. (author)Despite aerobic granular sludge wastewater treatment plants operating around the world, our understanding of internal granule structure and its relation to treatment efficiency remains limited. This can be attributed in part to the drawbacks of time-consuming, labor-intensive, and invasive microscopy protocols which effectively restrict...journal article 2020
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Soler Jofra, A. (author), Wang, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)A lab-scale partial nitritation granular sludge air-lift reactor was operated in continuous mode treating low strength synthetic medium (influent ca. 50 mg-N-NH<sub>4</sub> <sup>+</sup>/L). Granules were initially stratified with AOB in the external shell and NOB in the inner core at 20 °C. Once temperature was decreased progressively from 20...journal article 2019
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Yavari, Mohsen (author), Ebrahimi, S. (author), Aghazadeh, Valeh (author), Ghashghaee, Mohammad (author)The relative performance of two biofilm-based airlift reactors using different kinds of packing materials and one fixed bed biofilm reactor with a homemade packing material of high specific area (~ 1000 m<sup>2</sup>/m<sup>3</sup>) was addressed. The bioreactors operated under ferrous iron loading rates in the range of 8–120 mol Fe(II)/m<sup...journal article 2019
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Felz, S. (author), Vermeulen, Pascalle (author), van Loosdrecht, Mark C.M. (author), Lin, Yue Mei (author)Biofilm structure and functionality depend on extracellular polymeric substances (EPS), but analytical methods for EPS often lack specificity which limits progress of biofilm research. EPS were extracted from aerobic granular sludge and analyzed with frequently applied colorimetric methods. The colorimetric methods were evaluated based on...journal article 2019
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