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Van Hoek, P. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 1998
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Mendes, F. (author), Sieuwerts, S. (author), De Hulster, E. (author), Almering, M.J. (author), Luttik, M.A. (author), Pronk, J.T. (author), Smid, E.J. (author), Bron, P.A. (author), Daran-Lapujade, P. (author)
Mixed populations of Saccharomyces cerevisiae yeasts and lactic acid bacteria occur in many dairy, food, and beverage fermentations, but knowledge about their interactions is incomplete. In the present study, interactions between Saccharomyces cerevisiae and Lactobacillus delbrueckii subsp. bulgaricus, two microorganisms that co-occur in kefir...
journal article 2013
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Livas, D. (author), Almering, M.J.H. (author), Daran, J.M. (author), Pronk, J.J. (author), Gancedo, J.M. (author)
Background The pattern of gene transcripts in the yeast Saccharomyces cerevisiae is strongly affected by the presence of glucose. An increased activity of protein kinase A (PKA), triggered by a rise in the intracellular concentration of cAMP, can account for many of the effects of glucose on transcription. In S. cerevisiae three genes, TPK1,...
journal article 2011
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Huisjes, E.H. (author), De Hulster, E. (author), Van Dam, J.C. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
The efficient fermentation of mixed substrates is essential for the microbial conversion of second-generation feedstocks, including pectin-rich waste streams such as citrus peel and sugar beet pulp. Galacturonic acid is a major constituent of hydrolysates of these pectin-rich materials. The yeast Saccharomyces cerevisiae, the main producer of...
journal article 2012
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Pronk, J.T. (author), Meijer, W.M. (author), Hazeu, W. (author), van Dijken, J.P. (author), Bos, P. (author), Kuenen, J.G. (author)
journal article 1991
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Zelle, R.M. (author), De Hulster, E. (author), Kloezen, W. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter?1 of malate at a yield of 0.42 mol (mol glucose)?1 in calcium carbonate-buffered shake flask cultures. With shake flasks, process parameters that are important for scaling up this...
journal article 2009
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Rebnegger, C. (author), Vos, T. (author), Graf, Alexandra B. (author), Valli, Minoska (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author), Mattanovicha, Diethard (author)
The yeast Pichia pastoris is a widely used host for recombinant protein production. Understanding its physiology at extremely low growth rates is a first step in the direction of decoupling product formation from cellular growth and therefore of biotechnological relevance. Retentostat cultivation is an excellent tool for studying microbes at...
journal article 2016
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Knijnenburg, T.A. (author), De Winde, J.H. (author), Daran, J.M. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), Reinders, M.J.T. (author), Wessels, L.F.A. (author)
journal article 2007
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Pronk, M. (author), Abbas, B. (author), Kleerebezem, R. (author), Van Loosdrecht, M.C.M. (author)
The influence of sludge age on granular sludge formation and microbial population dynamics in a methanol- and acetate-fed aerobic granular sludge system operated at 35°C was investigated. During anaerobic feeding of the reactor, methanol was initially converted to methane by methylotrophic methanogens. These methanogens were able to withstand...
journal article 2015
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Daran, J.M. (author), Bakker, B.M. (author), Pronk, J.T. (author), Westerhoff, H.V. (author), De Winde, J.H. (author), Heck, A.J.R. (author), Slijper, M. (author), De Groot, M.J.L. (author), Luttik, M.A.H. (author), Van Gulik, W.M. (author), Rossell, S. (author), Daran-Lapujade, P. (author)
Metabolic fluxes may be regulated ‘‘hierarchically,’’ e.g., by changes of gene expression that adjust enzyme capacities (Vmax) and/or ‘‘metabolically’’ by interactions of enzymes with substrates, products, or allosteric effectors. In the present study, a method is developed to dissect the hierarchical regulation into contributions by...
journal article 2007
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Papapetridis, I. (author), van Dijk, M. (author), Dobbe, Arthur P A (author), Metz, B. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)
Background: Acetic acid, an inhibitor of sugar fermentation by yeast, is invariably present in lignocellulosic hydrolysates which are used or considered as feedstocks for yeast-based bioethanol production. Saccharomyces cerevisiae strains have been constructed, in which anaerobic reduction of acetic acid to ethanol replaces glycerol formation...
journal article 2016
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Wisselink, H.W. (author), Toirkens, M.J. (author), Wu, Q. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Lignocellulosic feedstocks are thought to have great economic and environmental significance for future biotechnological production processes. For cost-effective and efficient industrial processes, complete and fast conversion of all sugars derived from these feedstocks is required. Hence, simultaneous or fast sequential fermentation of sugars...
journal article 2008
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Pronk, J.T. (author), Levering, P.R. (author), Oiijve, W. (author), Van Dijken, J.P. (author)
journal article 1989
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Vos, T. (author), De la Torre Cortes, P. (author), Van Gulik, W.M. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)
Introduction: Saccharomyces cerevisiae has become a popular host for production of non-native compounds. The metabolic pathways involved generally require a net input of energy. To maximize the ATP yield on sugar in S. cerevisiae, industrial cultivation is typically performed in aerobic, sugar-limited fed-batch reactors which, due to constraints...
journal article 2015
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Zelle, R.M. (author), Trueheart, J. (author), Harrison, J.C. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Pyruvate carboxylase is the sole anaplerotic enzyme in glucose-grown cultures of wild-type Saccharomyces cerevisiae. Pyruvate carboxylase-negative (Pyc–) S. cerevisiae strains cannot grow on glucose unless media are supplemented with C4 compounds, such as aspartic acid. In several succinate-producing prokaryotes, phosphoenolpyruvate...
journal article 2010
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Castrillo, J.I. (author), Kaliterna, J. (author), Weusthuis, R.A. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 1996
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Bakker, B.M. (author), Bro, C. (author), Kotter, P. (author), Luttik, M.A. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 2000
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Hensing, M.C.M. (author), Rouwenhorst, R.J. (author), Heijnen, J.J. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 1995
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Flikweert, M.T. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 1997
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Pronk, M. (author), Neu, Thomas R. (author), van Loosdrecht, M.C.M. (author), Lin, Y. (author)
A new acid soluble extracellular polymeric substance (acid soluble EPS) was extracted from an acetate fed aerobic granular sludge reactor operated at 35 °C. Acid soluble EPS dominated granules exhibited a remarkable and distinctive tangled tubular morphology. These granules are dominated by Defluviicoccus Cluster II organisms. Acetic acid...
journal article 2017
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