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Diderich, J.A. (author), Weening, S.M. (author), van den Broek, M.A. (author), Pronk, J.T. (author), Daran, J.G. (author)
Saccharomyces pastorianus is an interspecies hybrid between S. cerevisiae and S. eubayanus. The identification of the parental species of S. pastorianus enabled the de novo reconstruction of hybrids that could potentially combine a wide array of phenotypic traits. Lager yeasts are characterized by their inability to decarboxylate ferulic acid...
journal article 2018
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Daran, J.M. (author), Pronk, J.T. (author)
In this feature, leading researchers in the field of environmental microbiology speculate on the technical and conceptual developments that will drive innovative research and open new vistas over the next few years. For the better part of four decades, genetic engineering has relied on a universal toolbox containing three indispensable...
journal article 2012
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Kuijpers, N.G.A. (author), Chroumpi, S. (author), Vos, T. (author), Solis-Escalante, D. (author), Bosman, D. (author), Pronk, J.T. (author), Daran, J.G. (author), Daran-Lapujade, P.A.S. (author)
In vivo assembly of overlapping fragments by homologous recombination in Saccharomyces cerevisiae is a powerful method to engineer large DNA constructs. Whereas most in vivo assembly methods reported to date result in circular vectors, stable integrated constructs are often preferred for metabolic engineering as they are required for large-scale...
journal article 2013
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Salgueiro Machado, S. (author), Luttik, M.A.H. (author), van Dijken, J.P. (author), Jongejan, J.A. (author), Pronk, J.T. (author)
journal article 1995
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Vuralhan, Z. (author), Luttik, M.A. (author), Tai, S.L. (author), Boer, V.M. (author), Morais, M.A. (author), Schipper, D. (author), Almering, M.J. (author), Kotter, P. (author), Dickinson, J.R. (author), Daran, J.M. (author), Pronk, J.T. (author)
journal article 2005
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Lindhoud, S. (author), Dias Ribeiro de Carvalho, V.I. (author), Pronk, J.W. (author), Aubin-Tam, M.E. (author)
Challenges in purification and subsequent functionalization of membrane proteins often complicate their biochemical and biophysical characterization. Purification of membrane proteins generally involves replacing the lipids surrounding the protein with detergent molecules, which can affect protein structure and function. Recently, it was shown...
journal article 2016
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Abbott, D.A. (author), Zelle, R.M. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
To meet the demands of future generations for chemicals and energy and to reduce the environmental footprint of the chemical industry, alternatives for petrochemistry are required. Microbial conversion of renewable feedstocks has a huge potential for cleaner, sustainable industrial production of fuels and chemicals. Microbial production of...
journal article 2009
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Overkamp, K.M. (author), Bakker, B.M. (author), Kotter, P. (author), Luttik, M.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 2002
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Kozak, B.U. (author), Van Rossum, H.M. (author), Luttik, M.A.H. (author), Akeroyd, M. (author), Benjamin, K.R. (author), Wu, L. (author), De Vries, S. (author), Daran, J.M. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
The energetic (ATP) cost of biochemical pathways critically determines the maximum yield of metabolites of vital or commercial relevance. Cytosolic acetyl coenzyme A (acetyl-CoA) is a key precursor for biosynthesis in eukaryotes and for many industrially relevant product pathways that have been introduced into Saccharomyces cerevisiae, such as...
journal article 2014
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Hazelwood, L.A. (author), Walsh, M.C. (author), Luttik, M.A.H. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), Daran, J.M. (author)
OA Fund TU Delft Accumulation of glycogen and trehalose in nutrient-limited cultures of Saccharomyces cerevisiae is negatively correlated with the specific growth rate. Additionally, glucose-excess conditions (i.e., growth limitation by nutrients other than glucose) are often implicated in high-level accumulation of these storage carbohydrates....
journal article 2009
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van Hoek, P. (author), Flikweert, M.T. (author), van der Aart, Q.J.M. (author), Steensma, H.Y. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 1998
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Jürgens, H. (author), Varela, Javier A. (author), Gorter de Vries, A.R. (author), Perli, T. (author), Gast, V.J.M. (author), Gyurchev, N.Y. (author), Rajkumar, Arun S. (author), Mans, R. (author), Pronk, J.T. (author), Morrissey, John P. (author), Daran, J.G. (author)
While CRISPR-Cas9-mediated genome editing has transformed yeast research, current plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR tools that function in multiple yeast species could contribute to the intensifying research on non-conventional yeasts. A plasmid carrying a pangenomic origin of replication...
journal article 2018
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Flikweert, M.T. (author), van der Zanden, L. (author), Janssen, W.M.Th.M. (author), Steensma, H.Y. (author), van Dijken, J.P. (author), Pronk, J.T. (author)
journal article 1996
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Meulenberg, R. (author), Pronk, J.T. (author), Hazeu, W. (author), Bos, P. (author), Kuenen, J.G. (author)
journal article 1992
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Milne, N. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
Background: Decarboxylation of ?-ketoisovalerate to isobutyraldehyde is a key reaction in metabolic engineering of Saccharomyces cerevisiae for isobutanol production with published studies relying on overexpression of either the native ARO10 gene or of the Lactococcus lactis kivD decarboxylase gene resulting in low enzymatic activities. Here, we...
journal article 2015
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Pronk, J.T. (author)
journal article 2002
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Veiga, T. (author), Nijland, J.G. (author), Driessen, A.J.M. (author), Bovenberg, R.A.L. (author), Touw, H. (author), Van den Berg, M.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
The multicomponent global regulator Velvet complex has been identified as a key regulator of secondary metabolite production in Aspergillus and Penicillium species. Previous work indicated a massive impact of PcvelA and PclaeA deletions on penicillin production in prolonged batch cultures of P. chrysogenum, as well as substantial changes in...
journal article 2012
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Pronk, J.T. (author), Van Schie, B.J. (author), Van Dijken, J.P. (author), Kuenen, J.G. (author)
journal article 1985
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Van Schie, B.J. (author), Pronk, J.T. (author), Hellingwerf, K.J. (author), Van Dijken, J.P. (author), Kuenen, J.G. (author)
journal article 1987
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Marques, W.L. (author), van der Woude, L.N. (author), Luttik, M.A.H. (author), van den Broek, M.A. (author), Nijenhuis, J.M. (author), Pronk, J.T. (author), van Maris, A.J.A. (author), Mans, R. (author), Gombert, Andreas K. (author)
Knowledge on the genetic factors important for the efficient expression of plant transporters in yeast is still very limited. Phaseolus vulgaris sucrose facilitator 1 (PvSuf1), a presumable uniporter, was an essential component in a previously published strategy aimed at increasing ATP yield in Saccharomyces cerevisiae. However, attempts to...
journal article 2018
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