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Daran, J.M. (author), Pronk, J.T. (author)In this feature, leading researchers in the field of environmental microbiology speculate on the technical and conceptual developments that will drive innovative research and open new vistas over the next few years. For the better part of four decades, genetic engineering has relied on a universal toolbox containing three indispensable...journal article 2012
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- Pronk, J.T. (author), Steensma, H.Y. (author), van Dijken, J.P. (author) journal article 1996
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Milne, N. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)Background: Decarboxylation of ?-ketoisovalerate to isobutyraldehyde is a key reaction in metabolic engineering of Saccharomyces cerevisiae for isobutanol production with published studies relying on overexpression of either the native ARO10 gene or of the Lactococcus lactis kivD decarboxylase gene resulting in low enzymatic activities. Here, we...journal article 2015
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Calderon Franco, D. (author), Sarelse, R.G. (author), Christou, S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Abeel, T.E.P.M.F. (author), Weissbrodt, D.G. (author)In the One Health context, wastewater treatment plants (WWTPs) are central to safeguarding water resources. Nonetheless, many questions remain about their effectiveness in preventing antimicrobial resistance (AMR) dissemination. Most surveillance studies monitor the levels and removal of selected antibiotic resistance genes (ARGs) and mobile...journal article 2022
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Pronk, Arno (author), Brancart, S. (author), Sanders, Fred (author)In 2018, the construction sector was responsible for 39% of the worldwide energy and process-related carbon dioxide emissions (Global Alliance for Buildings and Construction et al., 2019). This is partly due to the embodied carbon, which represents the carbon emissions related to building construction and material production (LETI, 2020). While...journal article 2022
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Bouwknegt, J. (author), Koster, C.C. (author), Vos, A.M. (author), Ortiz Merino, R.A. (author), Wassink, Mats (author), Luttik, M.A.H. (author), van den Broek, M.A. (author), Hagedoorn, P.L. (author), Pronk, J.T. (author)Following publication of the original article [1], the authors reported errors in the text of the Results section and in Table 2. It refers to a mutation in a yeast gene as VPS1<sup>I410L</sup> and to the corresponding change in the Vps1 amino-acid sequence as I410L. The correct descriptions should read VPS1<sup>I401L</sup> and I401L,...journal article 2021
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Pronk, J. B. (author), Bolch, T. (author), King, O. (author), Wouters, B. (author), Benn, D. I. (author)Meltwater from Himalayan glaciers sustains the flow of rivers such as the Ganges and Brahmaputra on which over half a billion people depend for day-to-day needs. Upstream areas are likely to be affected substantially by climate change, and changes in the magnitude and timing of meltwater supply are expected to occur in coming decades. About 10 %...journal article 2021
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Hakkaart, X.D.V. (author), Pronk, J.T. (author), van Maris, A.J.A. (author)Understanding microbial growth and metabolism is a key learning objective of microbiology and biotechnology courses, essential for understanding microbial ecology, microbial biotechnology and medical microbiology. Chemostat cultivation, a key research tool in microbial physiology that enables quantitative analysis of growth and metabolism under...journal article 2017
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Elahinik, A. (author), Haarsma, M.N. (author), Abbas, B.A. (author), Pabst, Martin (author), Xevgenos, Dimitris (author), van Loosdrecht, Mark C.M. (author), Pronk, M. (author)Glycerol is abundantly present in wastewater from industries such as biodiesel production facilities. Glycerol is also a potential carbon source for microbes that are involved in wastewater nutrient removal processes. The conversion of glycerol in biological phosphorus removal of aerobic granular sludge processes has not been explored to date....journal article 2022
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Brouwers, N. (author), Gorter de Vries, A.R. (author), van den Broek, M.A. (author), Weening, S.M. (author), Elink Schuurman, Tom D. (author), Kuijpers, Niels G.A. (author), Pronk, J.T. (author), Daran, J.G. (author)Saccharomyces eubayanus is the non-S. cerevisiae parent of the lager-brewing hybrid S. pastorianus. In contrast to most S. cerevisiae and Frohberg-type S. pastorianus strains, S. eubayanus cannot utilize the α-tri-glucoside maltotriose, a major carbohydrate in brewer's wort. In Saccharomyces yeasts, utilization of maltotriose is encoded by...journal article 2019
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Roy, E.I. (author), Pronk, Maarten (author), Agugiaro, G. (author), Ledoux, H. (author)Data on the number of floors is required for several applications, for instance, energy demand estimation, population estimation, and flood response plans. Despite this, open data on the number of floors is very rare, even when a 3D city model is available. In practice, it is most often inferred with a geometric method: elevation data is used...journal article 2022
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Pronk, C. (author)Over the years engineers have dearched for means to display decimal numbers on measuring equipment. This paper presents an overview.journal article 2022
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Pronk, C. (author), Trimp, P.J. (author)A description of the working pricciples of the Esiac-10 Algebraic Computerjournal article 2022
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Mooiman, C. (author), Bouwknegt, J. (author), Dekker, W.J.C. (author), Wiersma, S.J. (author), Ortiz Merino, R.A. (author), de Hulster, A.F. (author), Pronk, J.T. (author)All known facultatively fermentative yeasts require molecular oxygen for growth. Only in a small number of yeast species, these requirements can be circumvented by supplementation of known anaerobic growth factors such as nicotinate, sterols and unsaturated fatty acids. Biosynthetic oxygen requirements of yeasts are typically small and,...journal article 2021
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Perli, T. (author), Vos, A.M. (author), Bouwknegt, J. (author), Dekker, W.J.C. (author), Wiersma, S.J. (author), Mooiman, C. (author), Ortiz Merino, R.A. (author), Daran, J.G. (author), Pronk, J.T. (author)Neocallimastigomycetes are unique examples of strictly anaerobic eukaryotes. This study investigates how these anaerobic fungi bypass reactions involved in synthesis of pyridine nucleotide cofactors and coenzyme A that, in canonical fungal pathways, require molecular oxygen. Analysis of Neocallimastigomycetes proteomes identified a candidate...journal article 2021
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- Abbott, DA (author), Zelle, RM (author), Pronk, J.T. (author), van Maris, A.J.A. (author) journal article 2009
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Brickwedde, A. (author), van den Broek, M.A. (author), Geertman, Jan Maarten A. (author), Magalhães, Frederico (author), Kuijpers, Niels G.A. (author), Gibson, Brian (author), Pronk, J.T. (author), Daran, J.G. (author)The lager brewing yeast Saccharomyces pastorianus, an interspecies hybrid of S. eubayanus and S. cerevisiae, ferments maltotriose, maltose, sucrose, glucose and fructose in wort to ethanol and carbon dioxide. Complete and timely conversion ("attenuation") of maltotriose by industrial S. pastorianus strains is a key requirement for process...journal article 2017
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Marques, W.L. (author), Mans, R. (author), Marella, Eko Roy (author), Cordeiro, Rosa Lorizolla (author), van den Broek, M.A. (author), Daran, J.G. (author), Pronk, J.T. (author), Gombert, Andreas K. (author), van Maris, A.J.A. (author)Many relevant options to improve efficacy and kinetics of sucrose metabolism in Saccharomyces cerevisiae and, thereby, the economics of sucrose-based processes remain to be investigated. An essential first step is to identify all native sucrose-hydrolysing enzymes and sucrose transporters in this yeast, including those that can be activated...journal article 2017
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Jansen, Mickel L.A. (author), Bracher, J.M. (author), Papapetridis, I. (author), Verhoeven, M.D. (author), de Bruijn, J.A. (author), de Waal, P. (author), van Maris, A.J.A. (author), Klaassen, P (author), Pronk, J.T. (author)The recent start-up of several full-scale ‘second generation’ ethanol plants marks a major milestone in the development of Saccharomyces cerevisiae strains for fermentation of lignocellulosic hydrolysates of agricultural residues and energy crops. After a discussion of the challenges that these novel industrial contexts impose on yeast strains,...journal article 2017
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Pronk, Tessa E. (author), Fischer, A. (author), van den Berg, Annemijne E.T. (author), Hofman, Roberta C.H.M. (author)A main focus of water managers with regard to micropollutants is the protection of aquatic ecology. However, micropollutants also have the potential to affect the production of clean drinking water. In this paper, we propose to consider the removal effort when assessing micro-pollutants with an ‘Effort Index’ (EI). Assessments using the EI...journal article 2023