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Bezzubik, V. (author), Belashenkov, N. (author), Vdovin, G.V. (author)New computational technique based on linear-scale differential analysis (LSDA) of digital image is proposed to find the best focus position in digital microscopy by means of defocus estimation in two near-focal positions only. The method is based on the calculation of local gradients of the image on different scales using its convolution with a...conference paper 2015