Searched for: author%3A%22Ganji%2C+M.%22
(1 - 7 of 7)
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Elbatsh, Ahmed M.O. (author), Kim, E. (author), Eeftens, J.M. (author), Raaijmakers, Jonne A. (author), van der Weide, Robin H. (author), García-Nieto, Alberto (author), Bravo, Sol (author), Ganji, M. (author), Dekker, C. (author)
Condensin is a conserved SMC complex that uses its ATPase machinery to structure genomes, but how it does so is largely unknown. We show that condensin's ATPase has a dual role in chromosome condensation. Mutation of one ATPase site impairs condensation, while mutating the second site results in hyperactive condensin that compacts DNA faster...
journal article 2019
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Ketterer, Philip (author), Ananth, A.N. (author), Laman Trip, J.D.S. (author), Mishra, Ankur (author), Bertosin, Eva (author), Ganji, M. (author), van der Torre, J. (author), Onck, Patrick (author), Dietz, Hendrik (author), Dekker, C. (author)
The nuclear pore complex (NPC) is the gatekeeper for nuclear transport in eukaryotic cells. A key component of the NPC is the central shaft lined with intrinsically disordered proteins (IDPs) known as FG-Nups, which control the selective molecular traffic. Here, we present an approach to realize artificial NPC mimics that allows controlling...
journal article 2018
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Ganji, M. (author), Shaltiel, Indra A. (author), Bisht, Shveta (author), Kim, E. (author), Kalichava, A. (author), Haering, Christian H. (author), Dekker, C. (author)
It has been hypothesized that SMC protein complexes such as condensin and cohesin spatially organize chromosomes by extruding DNA into large loops. We directly visualized the formation and processive extension of DNA loops by yeast condensin in real time. Our findings constitute unambiguous evidence for loop extrusion. We observed that a...
journal article 2018
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Kim, S.H. (author), Ganji, M. (author), Kim, E. (author), van der Torre, J. (author), Abbondanzieri, E. (author), Dekker, C. (author)
The three-dimensional organization of DNA is increasingly understood to play a decisive role in vital cellular processes. Many studies focus on the role of DNA-packaging proteins, crowding, and confinement in arranging chromatin, but structural information might also be directly encoded in bare DNA itself. Here, we visualize plectonemes ...
journal article 2018
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Ganji, M. (author)
doctoral thesis 2016
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Ganji, M. (author), Docter, M.W. (author), Le Grice, Stuart F J (author), Abbondanzieri, E. (author)
Finding the target site and associating in a specific orientation are essential tasks for DNA-binding proteins. In order to make the target search process as efficient as possible, proteins should not only rapidly diffuse to the target site but also dynamically explore multiple local configurations before diffusing away. Protein flipping is...
journal article 2016
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Ganji, M. (author), Kim, S.H. (author), van der Torre, J. (author), Abbondanzieri, E. (author), Dekker, C. (author)
DNA supercoiling crucially affects cellular processes such as DNA replication, gene expression, and chromatin organization. However, mechanistic understanding of DNA supercoiling and the related DNA-processing enzymes has remained limited, mainly due to the lack of convenient experimental tools to probe these phenomena. Here, we report a...
journal article 2016
Searched for: author%3A%22Ganji%2C+M.%22
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