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Damping of 3D-printed polymer microbeam resonators

de Winter, Jikke (author), Manzaneque Garcia, T. (author), Ghatkesar, M.K. (author)

The emerging high-resolution 3D printing technique called two-photon polymerization (2PP) enables to print devices bottom-up rapidly, contrary to the top-down lithography-based fabrication methods. In this work, various polymer microbeams are 3D printed and their resonant characteristics are analyzed to understand the origin of damping. The...

journal article 2024

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Microwell-enhanced optical rapid antibiotic susceptibility testing of single bacteria

Roslon, I.E. (author), Japaridze, A. (author), Rodenhuis, Stef (author), Hamoen, Lieke (author), Ghatkesar, M.K. (author), Steeneken, P.G. (author), Dekker, C. (author), Alijani, F. (author)

Bacteria that are resistant to antibiotics present an increasing burden on healthcare. To address this emerging crisis, novel rapid antibiotic susceptibility testing (AST) methods are eagerly needed. Here, we present an optical AST technique that can determine the bacterial viability within 1 h down to a resolution of single bacteria. The...

journal article 2023

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Resolution Limits of Resonant Sensors

Manzaneque Garcia, T. (author), Ghatkesar, M.K. (author), Alijani, F. (author), Xu, M. (author), Norte, R.A. (author), Steeneken, P.G. (author)

Resonant sensors hold great promise in measuring small masses, to enable future mass spectrometers, and small forces in applications like atomic and magnetic force microscopy. During the last decades, scaling down the size of resonators has led to huge enhancements in sensing resolution, but has also raised the question of what the ultimate...

journal article 2023

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Portable and integrated microfluidic flow control system using off-the-shelf components towards organs-on-chip applications

Zhu, Haoyu (author), Özkayar, G. (author), Lötters, J.C. (author), Tichem, M. (author), Ghatkesar, M.K. (author)

Organ-on-a-chip (OoC) devices require the precise control of various media. This is mostly done using several fluid control components, which are much larger than the typical OoC device and connected through fluidic tubing, i.e., the fluidic system is not integrated, which inhibits the system’s portability. Here, we explore the limits of...

journal article 2023

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Flow Ripple Reduction in Reciprocating Pumps by Multi-Phase Rectification

Özkayar, G. (author), Wang, Z. (author), Lötters, J.C. (author), Tichem, M. (author), Ghatkesar, M.K. (author)

Reciprocating piezoelectric micropumps enable miniaturization in microfluidics for lab-on-a-chip applications such as organs-on-chips (OoC). However, achieving a steady flow when using these micropumps is a significant challenge because of flow ripples in the displaced liquid, especially at low frequencies or low flow rates (<50 µL/min)....

journal article 2023

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Discrete Femtolitre Pipetting with 3D Printed Axisymmetrical Phaseguides

Blankespoor, Maarten (author), Manzaneque Garcia, T. (author), Ghatkesar, M.K. (author)

The capacity to precisely pipette femtoliter volumes of liquid enables many applications, for example, to functionalize a nanoscale surface and manipulate fluids inside a single-cell. A pressure-controlled pipetting method is the most preferred, since it enables the widest range of working liquids. However, precisely controlling femtoliter...

journal article 2023

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Ripple reduction in piezoelectric micropumps by phased actuation in parallel and damping

Özkayar, G. (author), Lötters, J.C. (author), Tichem, M. (author), Ghatkesar, M.K. (author)

Piezoelectric micropumps enable miniaturization in microfluidics for lab-on-a-chip applications such as Organs-on-chips (OoC). However, achieving a steady flow with these micropumps is a significant challenge because of the reciprocating motion of the displacing component. Although dampers are widely preferred for reducing ripples, they are not...

conference paper 2022

document

Measuring nanoparticles in liquid with attogram resolution using a microfabricated glass suspended microchannel resonator

Daryani, Mehdi Mollaie (author), Manzaneque Garcia, T. (author), Wei, J. (author), Ghatkesar, M.K. (author)

The use of nanoparticles has been growing in various industrial fields, and concerns about their effects on health and the environment have been increasing. Hence, characterization techniques for nanoparticles are essential. Here, we present a silicon dioxide microfabricated suspended microchannel resonator (SMR) to measure the mass and...

journal article 2022

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Fluidic Force Microscopy and Atomic Force Microscopy Unveil New Insights into the Interactions of Preosteoblasts with 3D-Printed Submicron Patterns

Angeloni, L. (author), Popa, Bogdan (author), Nouri Goushki, M. (author), Minneboo, M.B. (author), Zadpoor, A.A. (author), Ghatkesar, M.K. (author), Fratila-Apachitei, E.L. (author)

Physical patterns represent potential surface cues for promoting osteogenic differentiation of stem cells and improving osseointegration of orthopedic implants. Understanding the early cell–surface interactions and their effects on late cellular functions is essential for a rational design of such topographies, yet still elusive. In this work...

journal article 2022

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Toward a modular, integrated, miniaturized, and portable microfluidic flow control architecture for organs-on-chips applications

Özkayar, G. (author), Lötters, J.C. (author), Tichem, M. (author), Ghatkesar, M.K. (author)

Microfluidic organs-on-chips (OoCs) technology has emerged as the trend for in vitro functional modeling of organs in recent years. Simplifying the complexities of the human organs under controlled perfusion of required fluids paves the way for accurate prediction of human organ functionalities and their response to interventions like...

review 2022

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Reducing anionic surfactant adsorption using polyacrylate as sacrificial agent investigated by QCM-D

Liu, Z. (author), Hedayati, Pegah (author), Ghatkesar, M.K. (author), Sun, Weichao (author), Onay, Hayati (author), Groenendijk, Dirk (author), van Wunnik, Johannes (author), Sudhölter, Ernst J. R. (author)

Surfactant losses by adsorption to rock surfaces make surfactant-based enhanced oil recovery economically less feasible. We investigated polyacrylate (PA) as a sacrificial agent in the reduction of anionic surfactant adsorption with focus on calcite surfaces by using quartz crystal microbalance with dissipation monitoring. It was found that...

journal article 2021

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3D-Printed Submicron Patterns Reveal the Interrelation between Cell Adhesion, Cell Mechanics, and Osteogenesis

Nouri Goushki, M. (author), Angeloni, L. (author), Modaresifar, K. (author), Minneboo, M.B. (author), Boukany, P. (author), Mirzaali, Mohammad J. (author), Ghatkesar, M.K. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

The surface topography of implantable devices is of crucial importance for guiding the cascade of events that starts from the initial contact of the cells with the surface and continues until the complete integration of the device in its immediate environment. There is, however, limited quantitative information available regarding the...

journal article 2021

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On the Use of Black Ti as a Bone Substituting Biomaterial: Behind the Scenes of Dual-Functionality

Modaresifar, K. (author), Ganjian, M. (author), Angeloni, L. (author), Minneboo, M.B. (author), Ghatkesar, M.K. (author), Hagedoorn, P.L. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

Despite the potential of small-scale pillars of black titanium (bTi) for killing the bacteria and directing the fate of stem cells, not much is known about the effects of the pillars’ design parameters on their biological properties. Here, three distinct bTi surfaces are designed and fabricated through dry etching of the titanium, each...

journal article 2021

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Mechanical characterization of nanopillars by atomic force microscopy

Angeloni, L. (author), Ganjian, M. (author), Nouri Goushki, M. (author), Mirzaali, Mohammad J. (author), Hagen, C.W. (author), Zadpoor, A.A. (author), Fratila-Apachitei, E.L. (author), Ghatkesar, M.K. (author)

Micro- and nano-patterns are gaining increasing attraction in several fields ranging from nanoelectronics to bioengineering. The mechanical properties of the nanostructures (nanopillars, nanotubes, nanowires, etc.) are highly relevant for many applications but challenging to determine. Existing mechanical characterization methods require...

journal article 2021

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Second flexural and torsional modes of vibration in suspended microfluidic resonator for liquid density measurements

Belardinelli, P. (author), D Souza, Savio (author), Verlinden, E.J. (author), Wei, J. (author), Staufer, U. (author), Alijani, F. (author), Ghatkesar, M.K. (author)

Suspended microfluidic resonators enable detection of fluid density and viscosity with high sensitivity. Here, a two-legged suspended microchannel resonator that probes pico-litres of liquid is presented. The higher resonant modes (flexural and torsional) were explored for increased sensitivity and resolution. Unlike other reported microchannel...

journal article 2020

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Method to Determine the Closed-Loop Precision of Resonant Sensors from Open-Loop Measurements

Manzaneque Garcia, T. (author), Steeneken, P.G. (author), Alijani, F. (author), Ghatkesar, M.K. (author)

Resonant sensors determine a sensed parameter by measuring the resonance frequency of a resonator. For fast continuous sensing, it is desirable to operate resonant sensors in a closed-loop configuration, where a feedback loop ensures that the resonator is always actuated near its resonance frequency, so that the precision is maximized even in...

journal article 2020

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Deciphering the Roles of Interspace and Controlled Disorder in the Bactericidal Properties of Nanopatterns against Staphylococcus aureus

Modaresifar, K. (author), Kunkels, Lorenzo (author), Ganjian, M. (author), Tümer, N. (author), Hagen, C.W. (author), Otten, L.G. (author), Hagedoorn, P.L. (author), Angeloni, L. (author), Ghatkesar, M.K. (author), Fratila-Apachitei, E.L. (author), Zadpoor, A.A. (author)

Recent progress in nano-/micro-fabrication techniques has paved the way for the emergence of synthetic bactericidal patterned surfaces that are capable of killing the bacteria via mechanical mechanisms. Different design parameters are known to affect the bactericidal activity of nanopatterns. Evaluating the effects of each parameter, isolated...

journal article 2020

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Mechanics of bioinspired functionally graded soft-hard composites made by multi-material 3D printing

Mirzaali, Mohammad J. (author), Herranz de la Nava, A. (author), Gunashekar, D. (author), Nouri Goushki, M. (author), Veeger, R. P.E. (author), Grossman, Q. (author), Angeloni, L. (author), Ghatkesar, M.K. (author), Fratila-Apachitei, E.L. (author), Ruffoni, D. (author), Doubrovski, E.L. (author), Zadpoor, A.A. (author)

Functional gradients are material transitions that are found in nature and are known to result in materials with superior properties and multiple functionalities. The emerging multi-material 3D printing (=additive manufacturing, AM) techniques provide a powerful tool for the design and fabrication of bioinspired functionally graded materials ...

journal article 2020

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Volume and concentration dosing in picolitres using a two-channel microfluidic AFM cantilever

Verlinden, E.J. (author), Madadelahi, M. (author), Sarajlic, E. (author), Shamloo, A. (author), Engel, A.H. (author), Staufer, U. (author), Ghatkesar, M.K. (author)

We introduce a two-channel microfluidic atomic force microscopy (AFM) cantilever that combines the nanomechanical sensing functionality of an AFM cantilever with the ability to manipulate fluids of picolitres or smaller volumes through nanoscale apertures near the cantilever tip. Each channel is connected to a separate fluid reservoir, which...

journal article 2020

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Multiscale 3D-printing of microfluidic AFM cantilevers

Kramer, R.C.L.N. (author), Verlinden, E.J. (author), Angeloni, L. (author), Van Den Heuvel, Anita (author), Fratila-Apachitei, E.L. (author), Van Der Maarel, Silvère M. (author), Ghatkesar, M.K. (author)

Microfluidic atomic force microscopy (AFM) cantilever probes have all the functionalities of a standard AFM cantilever along with fluid pipetting. They have a channel inside the cantilever and an aperture at the tip. Such probes are useful for precise fluid manipulation at a desired location, for example near or inside cells. They are...

journal article 2020