Searched for: author%3A%22Giepmans%2C+Ben+N.G.%22
(1 - 9 of 9)
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Kievits, A.J. (author), Duinkerken, B. H.Peter (author), Fermie, Job (author), Lane, R. (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Recent advances in electron microscopy techniques have led to a significant scale up in volumetric imaging of biological tissue. The throughput of electron microscopes, however, remains a limiting factor for the volume that can be imaged in high resolution within reasonable time. Faster detection methods will improve throughput. Here, we have...
journal article 2024
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Kievits, A.J. (author), Peter Duinkerken, B. H. (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
journal article 2023
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Lane, R. (author), Wolters, Anouk H.G. (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Volume electron microscopy (EM) of biological systems has grown exponentially in recent years due to innovative large-scale imaging approaches. As a standalone imaging method, however, large-scale EM typically has two major limitations: slow rates of acquisition and the difficulty to provide targeted biological information. We developed a 3D...
journal article 2022
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Faraj, Noura (author), Duinkerken, B. H.Peter (author), Carroll, E.C.M. (author), Giepmans, Ben N.G. (author)
Microscopic analysis of molecules and physiology in living cells and systems is a powerful tool in life sciences. While in vivo subcellular microscopic analysis of healthy and diseased human organs remains impossible, zebrafish larvae allow studying pathophysiology of many organs using in vivo microscopy. Here, we review the potential of the...
review 2022
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Lane, R. (author), Balkenende, L. (author), van Staalduine, S.E. (author), Wolters, Anouk H.G. (author), Giepmans, Ben N.G. (author), Voortman, Lennard (author), Hoogenboom, J.P. (author)
journal article 2022
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Lane, R. (author), Vos, Y. (author), Wolters, A.E. (author), van Kessel, L.C.P.M. (author), Chen, S. Elisa (author), Liv, Nalan (author), Klumperman, Judith (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging speed. Negative bias potential has previously...
journal article 2021
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Srinivasa Raja, A. (author), de Boer, Pascal (author), Giepmans, Ben N.G. (author), Hoogenboom, J.P. (author)
Electron microscopy is crucial for imaging biological ultrastructure at nanometer resolution. However, electron irradiation also causes specimen damage, reflected in structural and chemical changes that can give rise to alternative signals. Here, luminescence induced by electron-beam irradiation is reported across a range of materials widely...
journal article 2021
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Pirozzi, N.M. (author), Hoogenboom, J.P. (author), Giepmans, Ben N.G. (author)
Nanometer-scale identification of multiple targets is crucial to understand how biomolecules regulate life. Markers, or probes, of specific biomolecules help to visualize and to identify. Electron microscopy (EM), the highest resolution imaging modality, provides ultrastructural information where several subcellular structures can be readily...
journal article 2018
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Scotuzzi, M. (author), Kuipers, Jeroen (author), Wensveen, D.I. (author), De Boer, Pascal (author), Hagen, C.W. (author), Hoogenboom, J.P. (author), Giepmans, Ben N.G. (author)
Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting grey-scale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis ...
journal article 2017
Searched for: author%3A%22Giepmans%2C+Ben+N.G.%22
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