Searched for: author%3A%22Janissen%2C+R.%22
(1 - 13 of 13)
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Ryu, J.K. (author), Rah, S.H. (author), Janissen, R. (author), Kerssemakers, J.W.J. (author), Bonato, Andrea (author), Michieletto, Davide (author), Dekker, C. (author)
The condensin SMC protein complex organizes chromosomal structure by extruding loops of DNA. Its ATP-dependent motor mechanism remains unclear but likely involves steps associated with large conformational changes within the ∼50 nm protein complex. Here, using high-resolution magnetic tweezers, we resolve single steps in the loop extrusion...
journal article 2022
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Janissen, R. (author), Eslami Mossallam, B. (author), Artsimovitch, Irina (author), Depken, S.M. (author), Dekker, N.H. (author)
Pausing by bacterial RNA polymerase (RNAp) is vital in the recruitment of regulatory factors, RNA folding, and coupled translation. While backtracking and intra-structural isomerization have been proposed to trigger pausing, our mechanistic understanding of backtrack-associated pauses and catalytic recovery remains incomplete. Using high...
journal article 2022
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Kuijpers, L.C. (author), van Laar, T. (author), Janissen, R. (author), Dekker, N.H. (author)
Multiplexed single-molecule magnetic tweezers (MT) have recently been employed to probe the RNA synthesis dynamics of RNA-dependent RNA polymerases (RdRp). Here, we present a protocol for simultaneously probing the RNA synthesis dynamics of hundreds of single polymerases with MT. We describe the preparation of a dsRNA construct for probing...
journal article 2022
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Janissen, R. (author), Filonenko, G.A. (author)
Mechanophores are powerful molecular tools used to track bond rupture and characterize mechanical damage in polymers. The majority of mechanophores are known to respond to external stresses, and we report in this study the first precedent of a mechanochemical response to internal, residual stresses that accumulate during polymer vitrification...
journal article 2022
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Anbumani, Silambarasan (author), da Silva, Aldeliane M. (author), Carvalho, Isis G.B. (author), Fischer, Eduarda Regina (author), de Souza e Silva, Mariana (author), von Zuben, Antonio Augusto G. (author), Carvalho, Hernandes F. (author), de Souza, Alessandra A. (author), Janissen, R. (author), Cotta, Monica A. (author)
The morphological plasticity of bacteria to form filamentous cells commonly represents an adaptive strategy induced by stresses. In contrast, for diverse human and plant pathogens, filamentous cells have been recently observed during biofilm formation, but their functions and triggering mechanisms remain unclear. To experimentally identify...
journal article 2021
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Janissen, R. (author), Woodman, Andrew (author), Shengjuler, Djoshkun (author), Vallet, Thomas (author), Lee, Kuo Ming (author), Kuijpers, L.C. (author), Moustafa, Ibrahim M. (author), Fitzgerald, Fiona (author), Dekker, N.H. (author)
Viral RNA-dependent RNA polymerases (RdRps) are a target for broad-spectrum antiviral therapeutic agents. Recently, we demonstrated that incorporation of the T-1106 triphosphate, a pyrazine-carboxamide ribonucleotide, into nascent RNA increases pausing and backtracking by the poliovirus RdRp. Here, by monitoring enterovirus A-71 RdRp dynamics...
journal article 2021
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Lehner, B. (author), Benz, D. (author), Moshkalev, Stanislav A. (author), Meyer, Anne S. (author), Cotta, Monica A. (author), Janissen, R. (author)
Graphene oxide (GO) has immense potential for widespread use in diverse in vitro and in vivo biomedical applications owing to its thermal and chemical resistance, excellent electrical properties and solubility, and high surface-to-volume ratio. However, development of GO-based biological nanocomposites and biosensors has been hampered by its...
journal article 2021
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Ha, S. (author), Tang, Y. (author), van Oene, M.M. (author), Janissen, R. (author), Dries, R.M. (author), Solano Hermosilla, B.P. (author), Adam, A.J.L. (author), Dekker, N.H. (author)
Optical trapping of (sub)micron-sized particles is broadly employed in nanoscience and engineering. The materials commonly employed for these particles, however, have physical properties that limit the transfer of linear or angular momentum (or both). This reduces the magnitude of forces and torques, and the spatiotemporal resolution,...
journal article 2019
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Janissen, R. (author), Sahoo, Prasana K. (author), Santos, Clelton A. (author), Da Silva, Aldeliane M. (author), Von Zuben, Antonio A.G. (author), Souto, Denio E.P. (author), Costa, Alexandre D.T. (author), Celedon, Paola (author), Zanchin, Nilson I.T. (author), Almeida, Diogo B. (author), Oliveira, Douglas S. (author)
Electrically active field-effect transistors (FET) based biosensors are of paramount importance in life science applications, as they offer direct, fast, and highly sensitive label-free detection capabilities of several biomolecules of specific interest. In this work, we report a detailed investigation on surface functionalization and...
journal article 2017
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Dekker, N.H. (author), Ha, S. (author), Janissen, R. (author), Ussembayev, Y. (author), van Oene, M.M. (author), Solano Hermosilla, B.P. (author)
Titanium dioxide (TiO2) is a key component of diverse optical and electronic applications that exploit its exceptional material properties. In particular, the use of TiO2 in its single-crystalline phase can offer substantial advantages over its amorphous and polycrystalline phases for existing and yet-to-be-developed applications. However, the...
journal article 2016
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Sahoo, Prasana K. (author), Janissen, R. (author), Monteiro, Moniellen P. (author), Cavalli, Alessandro (author), Murillo, Duber M. (author), Merfa, Marcus V. (author), Cesar, Carlos L. (author), Carvalho, Hernandes F. (author), De Souza, Alessandra A. (author), Bakkers, E.P.A.M. (author), Cotta, Monica A. (author)
Surface attachment of a planktonic bacteria, mediated by adhesins and extracellular polymeric substances (EPS), is a crucial step for biofilm formation. Some pathogens can modulate cell adhesiveness, impacting host colonization and virulence. A framework able to quantify cell-surface interaction forces and their dependence on chemical surface...
journal article 2016
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Berghuis, B.A. (author), Dulin, D. (author), Xu, Zhi Qiang (author), van Laar, T. (author), Cross, B. (author), Janissen, R. (author), Jergic, Slobodan (author), Dixon, Nicholas E. (author), Depken, S.M. (author), Dekker, N.H. (author)
The bidirectional replication of a circular chromosome by many bacteria necessitates proper termination to avoid the head-on collision of the opposing replisomes. In Escherichia coli, replisome progression beyond the termination site is prevented by Tus proteins bound to asymmetric Ter sites. Structural evidence indicates that strand...
journal article 2015
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Janissen, R. (author), Berghuis, B.A. (author), Dulin, D. (author), Wink, M. (author), Van Laar, T. (author), Dekker, N.H. (author)
Magnetic tweezers are a powerful single-molecule technique that allows real-time quantitative investigation of biomolecular processes under applied force. High pulling forces exceeding tens of picoNewtons may be required, e.g. to probe the force range of proteins that actively transcribe or package the genome. Frequently, however, the...
journal article 2014
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