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Filius, M. (author), Kim, S.H. (author), Severins, I.W.H. (author), Joo, C. (author)Single-molecule FRET is a versatile tool to study nucleic acids and proteins at the nanometer scale. However, currently, only a couple of FRET pairs can be reliably measured on a single object, which makes it difficult to apply single-molecule FRET for structural analysis of biomolecules. Here, we present an approach that allows for the...journal article 2021
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van Wee, R.G. (author), Filius, M. (author), Joo, C. (author)Single-molecule localization microscopy (SMLM) is a potent tool to examine biological systems with unprecedented resolution, enabling the investigation of increasingly smaller structures. At the forefront of these developments is DNA-based point accumulation for imaging in nanoscale topography (DNA-PAINT), which exploits the stochastic and...review 2021
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Filius, M. (author), Cui, T.J. (author), Ananth, A.N. (author), Docter, M.W. (author), Hegge, Jorrit W. (author), van der Oost, John (author), Joo, C. (author)Super-resolution imaging allows for the visualization of cellular structures on a nanoscale level. DNA-PAINT (DNA point accumulation in nanoscale topology) is a super-resolution method that depends on the binding and unbinding of DNA imager strands. The current DNA-PAINT technique suffers from slow acquisition due to the low binding rate of...journal article 2020