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Rossi, E. (author), Vermeer, C.M. (author), Mors, R.M. (author), Kleerebezem, R. (author), Copuroglu, Oguzhan (author), Jonkers, H.M. (author)Bacteria-based self-healing concrete has the ability to heal cracks due to the bacterial conversion of incorporated organic compounds into calcium carbonate. Precipitates seal the cracks, theoretically increasing the service life of constructions. The aim of this paper is to propose a precursor for bacteria-based self-healing concrete derived...journal article 2021
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Kleerebezem, R. (author), Stouten, G.R. (author), Koehorst, Jasper (author), Langenhoff, Alette (author), Schaap, Peter (author), Smidt, Hauke (author)Natural microbial communities are composed of a large diversity of interacting microorganisms, each with a specific role in the functional properties of the ecosystem. The objectives in microbial ecology research are related to identifying, understanding and exploring the role of these different microorganisms. Because of the rapidly...review 2021
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Estevez Alonso, A. (author), Pei, R. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Conversion of organic waste and wastewater to polyhydroxyalkanoates (PHAs) offers a potential to recover valuable resources from organic waste. Microbial community-based PHA production systems have been successfully applied in the last decade at lab- and pilot-scales, with a total of 19 pilot installations reported in the scientific...review 2021
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Rombouts, J.L. (author), Kranendonk, Elsemiek Madeleine Maria (author), Regueira, Alberte (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Lactic acid-producing bacteria are important in many fermentations, such as the production of biobased plastics. Insight in the competitive advantage of lactic acid bacteria over other fermentative bacteria in a mixed culture enables ecology-based process design and can aid the development of sustainable and energy-efficient bioprocesses....journal article 2020
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Mulders, M. (author), Tamis, J. (author), Abbas, B.A. (author), Sousa, João (author), Dijkman, Henk (author), Rozendal, René (author), Kleerebezem, R. (author)Polyhydroxyalkanoate (PHA) accumulating microbial enrichment was established on volatile fatty acids (VFAs) containing leachate derived from the organic fraction of municipal solid waste (OFMSW). The enrichment was based on a 12-h feast-famine batch cycle and an exchange ratio of 50% in which VFAs were completely consumed in less than 50 min...journal article 2020
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Ceron Chafla, P.S. (author), Kleerebezem, R. (author), Rabaey, Korneel (author), van Lier, J.B. (author), Lindeboom, R.E.F. (author)Simultaneous digestion and in situ biogas upgrading in high-pressure bioreactors will result in elevated CO2 partial pressure (pCO2). With the concomitant increase in dissolved CO2, microbial conversion processes may be affected beyond the impact of increased acidity. Elevated pCO2 was reported to affect the kinetics and thermodynamics of...journal article 2020
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Mulders, M. (author), Tamis, J. (author), Stouten, G.R. (author), Kleerebezem, R. (author)The wide variety of organic carbon to nitrogen and phosphorous ratios that are encountered in different wastewaters has a major impact on the poly(3-hydroxybutyrate) (PHB) accumulation potential of microbial communities. In this study we investigated the influence of the substrate composition in terms of the carbon to nitrogen (C/N) or...journal article 2020
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Soler Jofra, A. (author), Wang, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)A lab-scale partial nitritation granular sludge air-lift reactor was operated in continuous mode treating low strength synthetic medium (influent ca. 50 mg-N-NH<sub>4</sub> <sup>+</sup>/L). Granules were initially stratified with AOB in the external shell and NOB in the inner core at 20 °C. Once temperature was decreased progressively from 20...journal article 2019
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Conthe Calvo, M. (author), Lycus, Pawel (author), Arntzen, Magnus (author), Ramos da Silva, Aline (author), Frostegård, Åsa (author), Bakken, Lars R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The strong greenhouse gas nitrous oxide (N<sub>2</sub>O) can be emitted from wastewater treatment systems as a byproduct of ammonium oxidation and as the last intermediate in the stepwise reduction of nitrate to N<sub>2</sub> by denitrifying organisms. A potential strategy to reduce N<sub>2</sub>O emissions would be to enhance the activity of...journal article 2019
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Rombouts, J.L. (author), Mos, G. (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)A mechanistic understanding of microbial community establishment and product formation in open fermentative systems can aid the development of bioprocesses utilising organic waste. Kinetically, a single rate-limiting substrate is expected to result in one dominant species. Four enrichment cultures were operated to ferment either xylose or...journal article 2019
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Rombouts, J.L. (author), Mos, Galvin (author), Weissbrodt, D.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Efficient industrial fermentation of lignocellulosic waste containing a large part of glucose and xylose is desirable to implement a circular economy. Mixed culture biotechnologies can aid in realizing this goal. The effect of feeding equivalent substrates to a microbial community, such a xylose and glucose, is not well understood in terms of...journal article 2019
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Conthe Calvo, M. (author), Wittorf, Lea (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Hallin, Sara (author)Reduction of the greenhouse gas N<sub>2</sub>O to N<sub>2</sub> is a trait among denitrifying and non-denitrifying microorganisms having an N<sub>2</sub>O reductase, encoded by nosZ. The nosZ phylogeny has two major clades, I and II, and physiological differences among organisms within the clades may affect N<sub>2</sub>O emissions from...journal article 2018
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Conthe Calvo, M. (author), Parchen, C.M. (author), Stouten, G.R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Despite its ecological importance, essential aspects of microbial N<sub>2</sub>O reduction—such as the effect of O<sub>2</sub> availability on the N<sub>2</sub>O sink capacity of a community—remain unclear. We studied N<sub>2</sub>O vs. aerobic respiration in a chemostat culture to explore (i) the extent to which simultaneous respiration of N...journal article 2018
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Hoekstra, M. (author), Geilvoet, Stefan P. (author), Hendrickx, Tim L.G. (author), van Erp Taalman Kip, Charlotte S. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)The aim of this research was to study the biological feasibility of the Partial Nitritation/Anammox (PN/A) technology to remove nitrogen from municipal mainstream wastewaters. During stable process operations at summer temperatures (23.2 ± 1.3°C), the total nitrogen removal rate was 0.223 ± 0.029 kg N (m<sup>3 </sup>d)<sup>−1</sup> while at...journal article 2018
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van Turnhout, A.G. (author), Brandstätter, Christian (author), Kleerebezem, R. (author), Fellner, Johann (author), Heimovaara, T.J. (author)Long-term emissions of Municipal Solid Waste (MSW) landfills are a burden for future generations because of the required long-term aftercare. To shorten aftercare, treatment methods have to be developed that reduce long-term emissions. A treatment method that reduces emissions at a lysimeter scale is re-circulation of leachate. However, its...journal article 2018
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Timmers, Peer H.A. (author), Vavourakis, Charlotte D. (author), Kleerebezem, R. (author), Sinninghe Damsté, Jaap S. (author), Muyzer, Gerard (author), Stams, Alfons J.M. (author), Sorokin, Dimitry Y. (author), Plugge, Caroline M. (author)Anaerobic syntrophic acetate oxidation (SAO) is a thermodynamically unfavorable process involving a syntrophic acetate oxidizing bacterium (SAOB) that forms interspecies electron carriers (IECs). These IECs are consumed by syntrophic partners, typically hydrogenotrophic methanogenic archaea or sulfate reducing bacteria. In this work, the...journal article 2018
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Conthe Calvo, M. (author), Wittorf, Lea (author), Kuenen, J.G. (author), Kleerebezem, R. (author), Hallin, Sara (author), van Loosdrecht, Mark C.M. (author)Nitrous oxide (N<sub>2</sub>O) reducing microorganisms may be key in the mitigation of N<sub>2</sub>O emissions from managed ecosystems. However, there is still no clear understanding of the physiological and bioenergetic implications of microorganisms possessing either of the two N<sub>2</sub>O reductase genes (nosZ), clade I and the more...journal article 2018
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Verhagen, K.J.A. (author), Gabriel Guedes da Silva, L. (author), van de Wijgaart, R. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Natural habitats of microorganisms are dynamic environments with non-continuous supply of carbon and energy sources, in which intermediate storage of substrates can increase competitiveness. Plasticicumulans acidivorans are polyhydroxybutyrate (PHB) accumulating bacteria enriched from activated sludge using carbon feast-famine cycles as...poster 2017
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van den Berg, E.M. (author), Perdigão Elisiário, M. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Denitrification and dissimilatory reduction to ammonium (DNRA) are competing nitrate-reduction processes that entail important biogeochemical consequences for nitrogen retention/removal in natural and man-made ecosystems. The nature of the available carbon source and electron donor have been suggested to play an important role on the outcome of...journal article 2017
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van den Berg, E.M. (author), Rombouts, J.L. (author), Kuenen, J.G. (author), Kleerebezem, R. (author), van Loosdrecht, Mark C.M. (author)Denitrification and dissimilatory nitrate reduction to ammonium (DNRA) are two microbial processes that compete for oxidized nitrogen compounds in the environment. The objective of this work was to determine the role of nitrite versus nitrate as terminal electron acceptor on the competition between DNRA and denitrification. Initially, a mixed...journal article 2017