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Modelling metastatic colonization of cholangiocarcinoma organoids in decellularized lung and lymph nodes

van Tienderen, Gilles S. (author), van Beek, M.E.A. (author), Schurink, Ivo J. (author), Rosmark, Oskar (author), Roest, Henk P. (author), Demmers, J.A.A. (author), Muntz, I.A.A. (author), Conboy, J.P. (author), Koenderink, G.H. (author)

Cholangiocarcinoma (CCA) is a type of liver cancer with an aggressive phenotype and dismal outcome in patients. The metastasis of CCA cancer cells to distant organs, commonly lung and lymph nodes, drastically reduces overall survival. However, mechanistic insight how CCA invades these metastatic sites is still lacking. This is partly because...

journal article 2023

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Sequence Control of the Self-Assembly of Elastin-Like Polypeptides into Hydrogels with Bespoke Viscoelastic and Structural Properties

López Barreiro, D. (author), Folch-Fortuny, Abel (author), Muntz, I.A.A. (author), Thies, Jens C. (author), Sagt, Cees M.J. (author), Koenderink, G.H. (author)

The biofabrication of structural proteins with controllable properties via amino acid sequence design is interesting for biomedicine and biotechnology, yet a complete framework that connects amino acid sequence to material properties is unavailable, despite great progress to establish design rules for synthesizing peptides and proteins with...

journal article 2023

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A systematic review and comparison of automated tools for quantification of fibrous networks

de Vries, J.J. (author), Laan, Daphne M. (author), Frey, F.F.F. (author), Koenderink, G.H. (author), de Maat, Moniek P.M. (author)

Fibrous networks are essential structural components of biological and engineered materials. Accordingly, many approaches have been developed to quantify their structural properties, which define their material properties. However, a comprehensive overview and comparison of methods is lacking. Therefore, we systematically searched for...

journal article 2023

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High-throughput mechanophenotyping of multicellular spheroids using a microfluidic micropipette aspiration chip

Boot, R.C. (author), Roscani, A.R. (author), van Buren, L. (author), Maity, Samadarshi (author), Koenderink, G.H. (author), Boukany, P. (author)

Cell spheroids are in vitro multicellular model systems that mimic the crowded micro-environment of biological tissues. Their mechanical characterization can provide valuable insights in how single-cell mechanics and cell-cell interactions control tissue mechanics and self-organization. However, most measurement techniques are limited to...

journal article 2023

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Steering self-organisation through confinement

Araújo, Nuno A.M. (author), Janssen, Liesbeth M.C. (author), Barois, Thomas (author), Boffetta, Guido (author), Cohen, Itai (author), Corbetta, Alessandro (author), Dauchot, Olivier (author), Dussutour, Audrey (author), Koenderink, G.H. (author)

Self-organisation is the spontaneous emergence of spatio-temporal structures and patterns from the interaction of smaller individual units. Examples are found across many scales in very different systems and scientific disciplines, from physics, materials science and robotics to biology, geophysics and astronomy. Recent research has...

review 2023

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Tumor decellularization reveals proteomic and mechanical characteristics of the extracellular matrix of primary liver cancer

van Tienderen, Gilles S. (author), Conboy, J.P. (author), Muntz, I.A.A. (author), Willemse, Jorke (author), Tieleman, Jantine (author), Monfils, Kathryn (author), Schurink, Ivo J. (author), Demmers, J.A.A. (author), Koenderink, G.H. (author)

Tumor initiation and progression are critically dependent on interaction of cancer cells with their cellular and extracellular microenvironment. Alterations in the composition, integrity, and mechanical properties of the extracellular matrix (ECM) dictate tumor processes including cell proliferation, migration, and invasion. Also in primary...

journal article 2023

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Exploring Giant Unilamellar Vesicle Production for Artificial Cells – Current Challenges and Future Directions

Van de Cauter, L. (author), van Buren, L. (author), Koenderink, G.H. (author), Ganzinger, Kristina A. (author)

Creating an artificial cell from the bottom up is a long-standing challenge and, while significant progress has been made, the full realization of this goal remains elusive. Arguably, one of the biggest hurdles that researchers are facing now is the assembly of different modules of cell function inside a single container. Giant unilamellar...

review 2023

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High-speed imaging of giant unilamellar vesicle production in cDICE

Van de Cauter, L. (author), Jawale, Y.K. (author), Tam, D.S.W. (author), Baldauf, L. (author), Koenderink, G.H. (author), Dogterom, A.M. (author), Ganzinger, Kristina A. (author)

journal article 2023

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Branched actin cortices reconstituted in vesicles sense membrane curvature

Baldauf, L. (author), Frey, F.F.F. (author), Arribas Perez, M. (author), Idema, T. (author), Koenderink, G.H. (author)

The actin cortex is a complex cytoskeletal machinery that drives and responds to changes in cell shape. It must generate or adapt to plasma membrane curvature to facilitate diverse functions such as cell division, migration, and phagocytosis. Due to the complex molecular makeup of the actin cortex, it remains unclear whether actin networks...

journal article 2023

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Fibrin clots from patients with acute-on-chronic liver failure are weaker than those from healthy individuals and patients with sepsis without underlying liver disease

Driever, Ellen G. (author), Muntz, I.A.A. (author), Patel, Vishal (author), Adelmeijer, Jelle (author), Bernal, William (author), Koenderink, G.H. (author), Lisman, Ton (author)

Background: Previous studies identified decreased clot permeability, without differences in fibrin fiber density in clots, from patients with cirrhosis compared with those from healthy controls (HCs). Fibrinogen hypersialylation could be the reason for this discrepancy. Objectives: The aim of this work was to study mechanical properties of...

journal article 2023

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Septin-microtubule association via a motif unique to isoform 1 of septin 9 tunes stress fibers

Kuzmić, Mira (author), Castro Linares, G. (author), Leischner Fialová, Jindřiška (author), Iv, François (author), Salaün, Danièle (author), Llewellyn, Alex (author), Gomes, Maxime (author), Belhabib, Mayssa (author), Koenderink, G.H. (author)

Septins, a family of GTP-binding proteins that assemble into higher order structures, interface with the membrane, actin filaments and microtubules, and are thus important regulators of cytoarchitecture. Septin 9 (SEPT9), which is frequently overexpressed in tumors and mutated in hereditary neuralgic amyotrophy (HNA), mediates the binding of...

journal article 2022

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The role of cell-matrix interactions in connective tissue mechanics

Muntz, I.A.A. (author), Fenu, M. (author), van Osch, G.J.V.M. (author), Koenderink, G.H. (author)

Living tissue is able to withstand large stresses in everyday life, yet it also actively adapts to dynamic loads. This remarkable mechanical behaviour emerges from the interplay between living cells and their non-living extracellular environment. Here we review recent insights into the biophysical mechanisms involved in the reciprocal...

review 2022

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Intermediate Filaments in Cellular Mechanoresponsiveness: Mediating Cytoskeletal Crosstalk From Membrane to Nucleus and Back

Ndiaye, A.B.E. (author), Koenderink, G.H. (author), Shemesh, M. (author)

The mammalian cytoskeleton forms a mechanical continuum that spans across the cell, connecting the cell surface to the nucleus via transmembrane protein complexes in the plasma and nuclear membranes. It transmits extracellular forces to the cell interior, providing mechanical cues that influence cellular decisions, but also actively generates...

review 2022

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Quantifying Cytoskeleton Dynamics Using Differential Dynamic Microscopy

Verwei, H.N. (author), Lee, Gloria (author), Leech, Gregor (author), Istúriz Petitjean, I. (author), Koenderink, G.H. (author), Robertson-Anderson, Rae M. (author), McGorty, Ryan James (author)

Cells can crawl, self-heal, and tune their stiffness due to their remarkably dynamic cytoskeleton. As such, reconstituting networks of cytoskeletal biopolymers may lead to a host of active and adaptable materials. However, engineering such materials with precisely tuned properties requires measuring how the dynamics depend on the network...

journal article 2022

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Purification and Quality Control of Recombinant Septin Complexes for Cell-Free Reconstitution

Castro Linares, G. (author), den Haan, J.C.A. (author), Iv, Francois (author), Martins, Carla Silva (author), Bertin, Aurélie (author), Mavrakis, Manos (author), Koenderink, G.H. (author)

Septins are a family of conserved eukaryotic GTP-binding proteins that can form cytoskeletal filaments and higher-order structures from hetero-oligomeric complexes. They interact with other cytoskeletal components and the cell membrane to participate in important cellular functions such as migration and cell division. Due to the complexity of...

journal article 2022

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DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles

van Buren, L. (author), Koenderink, G.H. (author), Martinez Torres, C.E. (author)

Giant unilamellar vesicles (GUVs) are cell-sized aqueous compartments enclosed by a phospholipid bilayer. Due to their cell-mimicking properties, GUVs have become a widespread experimental tool in synthetic biology to study membrane properties and cellular processes. In stark contrast to the experimental progress, quantitative analysis of GUV...

journal article 2022

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Actomyosin-Driven Division of a Synthetic Cell

Baldauf, L. (author), van Buren, L. (author), Fanalista, F. (author), Koenderink, G.H. (author)

One of the major challenges of bottom-up synthetic biology is rebuilding a minimal cell division machinery. From a reconstitution perspective, the animal cell division apparatus is mechanically the simplest and therefore attractive to rebuild. An actin-based ring produces contractile force to constrict the membrane. By contrast, microbes and...

review 2022

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Cross-linkers at growing microtubule ends generate forces that drive actin transport

Alkemade, C. (author), Wierenga, H. (author), Volkov, V. (author), López, Magdalena Preciado (author), Akhmanova, Anna (author), ten Wolde, Pieter Rein (author), Dogterom, A.M. (author), Koenderink, G.H. (author)

The actin and microtubule cytoskeletons form active networks in the cell that can contract and remodel, resulting in vital cellular processes such as cell division and motility. Motor proteins play an important role in generating the forces required for these processes, but more recently the concept of passive cross-linkers being able to...

journal article 2022

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Swelling-Dependent Shape-Based Transformation of a Human Mesenchymal Stromal Cells-Laden 4D Bioprinted Construct for Cartilage Tissue Engineering

Diaz Payno, P.J. (author), Kalogeropoulou, Maria (author), Muntz, I.A.A. (author), Kingma, Esther (author), Kops, Nicole (author), D'Este, Matteo (author), Koenderink, G.H. (author), Fratila-Apachitei, E.L. (author), van Osch, G.J.V.M. (author), Zadpoor, A.A. (author)

3D bioprinting is usually implemented on flat surfaces, posing serious limitations in the fabrication of multilayered curved constructs. 4D bioprinting, combining 3D bioprinting with time-dependent stimuli-induced transformation, enables the fabrication of shape-changing constructs. Here, a 4D biofabrication method is reported for cartilage...

journal article 2022

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Bottom-Up In Vitro Methods to Assay the Ultrastructural Organization, Membrane Reshaping, and Curvature Sensitivity Behavior of Septins

Chauvin, Brieuc (author), Nakazawa, Koyomi (author), Beber, Alexandre (author), Di Cicco, Aurélie (author), Hajj, Bassam (author), Iv, François (author), Mavrakis, Manos (author), Koenderink, G.H. (author), Cabral, João T. (author)

Membrane remodeling occurs constantly at the plasma membrane and within cellular organelles. To fully dissect the role of the environment (ionic conditions, protein and lipid compositions, membrane curvature) and the different partners associated with specific membrane reshaping processes, we undertake in vitro bottom-up approaches. In recent...

journal article 2022