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Totlani, K. (author), van Tatenhove-Pel, R.J. (author), Kreutzer, M.T. (author), van Gulik, W.M. (author), van Steijn, V. (author)It is common practice in the development of bioprocesses to genetically modify a microorganism and study a large number of resulting mutants in order to select the ones that perform best for use at the industrial scale. At industrial scale, strict nutrient-controlled growth conditions are imposed to control the metabolic activity and growth...review 2023
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Benz, D. (author), Van Bui, Hao (author), Hintzen, H.T.J.M. (author), Kreutzer, M.T. (author), van Ommen, J.R. (author)The photocatalytic mechanism of TiO<sub>2</sub> (P25) nanoparticles coated with SiO<sub>2</sub> (SiO<sub>2</sub>:TiO<sub>2</sub>) by atomic layer deposition was investigated. The deposition of SiO<sub>2</sub> on TiO<sub>2</sub> not only gives a photocatalytic improvement for the degradation of both Rhodamine B (3.6–fold) and Acid Blue 9 (3...journal article 2022
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Shah, M.S. (author), Kleijn, C.R. (author), Kreutzer, M.T. (author), van Steijn, V. (author)The initial thickness and radius of the film that forms upon close contact of two foam bubbles are known to influence the thinning dynamics and lifetime of the film. Various scalings of lifetime tr, with initial radius Rfilm and thickness ho, have been proposed in literature. In this paper, we present a hydrodynamic thin-film model that...journal article 2021
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Muralidharan, A. (author), Rems, Lea (author), Kreutzer, M.T. (author), Boukany, P. (author)Transient physical disruption of cell membranes by electric pulses (or electroporation) has significance in biomedical and biological applications requiring the delivery of exogenous (bio)molecules to living cells. We demonstrate that actin networks regulate the cell membrane permeability during electroporation. Disruption of actin networks...journal article 2021
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Totlani, K. (author), Wang, Yen Chieh (author), Bisschops, Maxime (author), de Riese, Thorben (author), Kreutzer, M.T. (author), van Gulik, W.M. (author), van Steijn, V. (author)A key bottleneck in bioprocess development is that state-of-the-art tools used for screening of cells and optimization of cultivation conditions do not represent the conditions enforced at industrial scale. At industrial scale, cell growth is strictly controlled (“fed-batch”) to optimize the metabolites produced by the cells. In contrast,...journal article 2021
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Sachdev, S. (author), Feijoo Moreira, Sara (author), Keehnen, Yasmine (author), Rems, L. (author), Kreutzer, M.T. (author), Boukany, P. (author)Size of DNA molecules governs their interaction with the cell membrane during electroporation and their subsequent transport inside the cell. In order to investigate the effect of DNA size on DNA-membrane interaction during electroporation, cells are electro-pulsed with DNA molecules; 15 bp, 25 bp, 50 bp, 100 bp and 1000 bp (bp = base pairs)....journal article 2020
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Totlani, K. (author), Hurkmans, Jan Willem (author), van Gulik, W.M. (author), Kreutzer, M.T. (author), van Steijn, V. (author)We developed a microfluidic droplet on-demand (DoD) generator that enables the production of droplets with a volume solely governed by the geometry of the generator for a range of operating conditions. The prime reason to develop this novel type of DoD generator is that its robustness in operation enables scale out and operation under non...journal article 2020
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Benz, D. (author), Felter, K.M. (author), Köser, Jan (author), Thöming, Jorg (author), Mul, Guido (author), Grozema, F.C. (author), Hintzen, H.T.J.M. (author), Kreutzer, M.T. (author), van Ommen, J.R. (author)The role of Pt on photocatalytic substrates such as TiO<sub>2</sub> (P25) for the decomposition of organic pollutants is still controversial in the scientific community. The well-observed behavior of an optimum catalytic activity as a function of the Pt loading is usually explained by the shift from charge separation to charge recombination...journal article 2020
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Benz, D. (author), Bui, Hao Van (author), Hintzen, H.T.J.M. (author), Kreutzer, M.T. (author), van Ommen, J.R. (author)Photocatalysts for water purification typically lack efficiency for practical applications. Here we present a multi-component (Pt:SiO<sub>2</sub>:TiO<sub>2</sub> (P25)) material that was designed using knowledge of reaction mechanisms of mono-modified catalysts (SiO<sub>2</sub>:TiO<sub>2</sub>, and Pt:TiO<sub>2</sub> ) combined with the...journal article 2020
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Sachdev, S. (author), Muralidharan, A. (author), Choudhary, Dipendra K. (author), Perrier, D.L. (author), Rems, L. (author), Kreutzer, M.T. (author), Boukany, P. (author)Delivery of naked DNA molecules into living cells via physical disruption of the membrane under electric pulses has potential biomedical applications ranging from gene electro-transfer, electro-chemotherapy, to gene therapy, yet the mechanisms involved in DNA transport remain vague. To investigate the mechanism of DNA translocation across the...journal article 2019
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Shah, M.S. (author), van Steijn, V. (author), Kleijn, C.R. (author), Kreutzer, M.T. (author)Thermal fluctuations have been shown to influence the thinning dynamics of planar thin liquid films, bringing predicted rupture times closer to experiments. Most liquid films in nature and industry are, however, non-planar. Thinning of such films not just results from the interplay between stabilizing surface tension forces and destabilizing...journal article 2019
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Márquez, Nathalie (author), Moulijn, J.A. (author), Makkee, M. (author), Kreutzer, M.T. (author), Castaño, Pedro (author)We describe the co-current flow pattern of gas and liquid through micro-fabricated beds of solid and pillars under variable (i) capillary number, (ii) contact angle or wettability and (iii) pillar arrangement, i.e. modifying the distance between pillars or their size and comparing regular with more chaotic systems. Laser-induced fluorescent...journal article 2019
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Visan, Aura (author), van Ommen, J.R. (author), Kreutzer, M.T. (author), Lammertink, Rob G.H. (author)This review addresses the inconsistencies in interpreting measurements of intrinsic catalyst properties using lab-scale devices. Any experiment must be analyzed in the framework of a model for which the choice and assumptions regarding the necessary parameters must be based on critical reasoning. Physical intuition about the properties of the...journal article 2019
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Haringa, C. (author), De Jong, Conrad (author), Hoang, D. (author), Portela, L. (author), Kleijn, C.R. (author), Kreutzer, M.T. (author), van Steijn, V. (author)We show experimentally, and explain theoretically, what velocity is needed to break an elongated droplet entering a microfluidic T-junction. Our experiments on short droplets confirm previous experimental and theoretical work that shows that the critical velocity for breakup scales with the inverse of the length of the droplet raised to the...journal article 2019
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Lovrak, M. (author), Hendriksen, W.E.J. (author), Kreutzer, M.T. (author), van Steijn, V. (author), Eelkema, R. (author), van Esch, J.H. (author)Controlled diffusion, reaction and assembly of hydrogelator precursors can be used to create soft hydrogel objects of defined shape and size. In this study we show that controlling local reaction kinetics by means of pH, diffusion length and the concentrations of reactants allows control over the dimensions of formed supramolecular structures....journal article 2019
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Perrier, D.L. (author), Vahid Belarghou, A. (author), Kathavi, Vaishnavi (author), Stam, Lotte (author), Rems, L. (author), Mulla, Yuval (author), Muralidharan, A. (author), Koenderink, Gijsje H. (author), Kreutzer, M.T. (author), Boukany, P. (author)We study the role of a biomimetic actin network during the application of electric pulses that induce electroporation or electropermeabilization, using giant unilamellar vesicles (GUVs) as a model system. The actin cortex, a subjacently attached interconnected network of actin filaments, regulates the shape and mechanical properties of the...journal article 2019
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Kreutzer, M.T. (author), Shah, M.S. (author), Parthiban, Pravien (author), Khan, S.A. (author)We experimentally and theoretically describe the dynamics of evolution and eventual rupture of Landau-Levich-Bretherton films of partially wetting liquids in microchannels in terms of nonplanar interface curvatures and disjoining pressure. While both the early-stage dynamics of film evolution and near-collapse dynamics of rupture are...journal article 2018
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Grillo, F. (author), La Zara, D. (author), Mulder, P.C.M. (author), Kreutzer, M.T. (author), Ruud Van Ommen, J. (author)Understanding the spontaneous organization of atoms on well-defined surfaces promises to enable control over the shape and size of supported nanostructures. Atomic layer deposition (ALD) boasts atomic-scale control in the synthesis of thin films and nanoparticles. Yet, the possibility to control the shape of ALD-grown nanostructures remains...journal article 2018
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Perrier, D.L. (author), Rems, L. (author), Kreutzer, M.T. (author), Boukany, P. (author)Transient permeabilisation of the cell membrane is a critical step to introduce drugs or DNA into living cells, yet challenging for both biological research and therapeutic applications. To achieve this, electroporation (or electropermeabilisation) has become a widely used method due to its simplicity to deliver almost any biomolecule to any...journal article 2018
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Kawale, D. (author), Bouwman, G.W. (author), Sachdev, S. (author), Zitha, P.L.J. (author), Kreutzer, M.T. (author), Rossen, W.R. (author), Boukany, P. (author)Molecular conformations of individual polymers during flow through porous media are directly observed by single-DNA imaging in microfluidics. As the Weissenberg number increases during flow (Wi > 1), we observe two types of elastic instabilities: (a) stationary dead-zone and (b) time-dependant dead-zone washing. When stretched polymer chains...journal article 2017