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van Campenhout, O.W.G. (author), van Nesselrooij, M. (author), Lin, Y. (author), Casacuberta Puig, J. (author), van Oudheusden, B.W. (author), Hickel, S. (author)Although several previous studies have reported a potential drag-reducing effect of dimpled surfaces in turbulent boundary layers, there is a lack of replicability across experiments performed by different research groups. To contribute to the dialogue, we scrutinize one of the most studied dimple geometries reported in the literature, which...journal article 2023
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Prest, Emmanuelle I. (author), Martijn, Bram J. (author), Rietveld, Matthijs (author), Lin, Y. (author), Schaap, Peter G. (author)Sediment formation in drinking water distribution systems can lead to brown water at customer taps. Previous studies have shown that sediment formation is closely linked with (micro)biological processes in the distribution system, however the mechanism is not fully understood. Most available studies on discoloration or sediment formation...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Li, Zejiao (author), Wang, Jixiang (author), Liu, Jialin (author), Chen, Xingyu (author), Lei, Zhongfang (author), Yuan, Tian (author), Lee, Duu Jong (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)Reducing CO<sub>2</sub> emission and energy consumption is crucial for the sustainable management of wastewater treatment plants (WWTPs). In this study, an algal-bacterial aerobic granular sludge (AGS) system was developed for efficient carbon (C) assimilation and nitrogen (N)/phosphorus (P) removal without the need for mechanical aeration....journal article 2023
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Chen, L.M. (author), de Bruin, S. (author), Pronk, M. (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Anaerobic and aerobic granular sludge processes are widely applied in wastewater treatment. In these systems, microorganisms grow in dense aggregates due to the production of extracellular polymeric substances (EPS). This study investigates the sialylation and sulfation of anionic glyconconjugates in anaerobic and aerobic granular sludges...journal article 2023
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Lin, Y. (author)The humanly constructed world is well-organized in space. A prominent feature of this artificial world is the presence of repetitive structures and coherent patterns, such as lines, junctions, wireframes of a building, and footprints of a city. These structures and patterns facilitate visual scene understanding by providing abundant geometry...doctoral thesis 2022
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de Bruin, S. (author), Vasquez Cardenas, D. (author), Sarbu, S. M. (author), Meysman, F.J.R. (author), Sousa, D. Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sulfated glycosaminoglycans (sGAG) are negatively charged extracellular polymeric substances that occur in biofilms from various environments. Yet, it remains unclear whether these polymers are acquired from the external environment or produced by microbes in the biofilm. To resolve this, we analyzed the presence of sGAGs in samples of an...journal article 2022
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Chen, Xingyu (author), Lee, Yu Jen (author), Yuan, Tian (author), Lei, Zhongfang (author), Adachi, Yasuhisa (author), Zhang, Zhenya (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)A reasonable recovery of excess sludge may shift the waste into wealth. Recently an increasing attention has been paid to the recycling of extracellular biopolymers from conventional and advanced biological wastewater treatment systems such as flocculent activated sludge (AS), bacterial aerobic granular sludge (AGS), and algal-bacterial AGS...review 2022
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Tomas Martinez, S. (author), Chen, Le Min (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Abstract: Pseudaminic and legionaminic acids are a subgroup of nonulosonic acids (NulOs) unique to bacterial species. There is a lack of advances in the study of these NulOs due to their complex synthesis and production. Recently, it was seen that “Candidatus Accumulibacter” can produce Pse or Leg analogues as part of its extracellular...journal article 2022
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Lin, Y. (author), Wiersma, R.T. (author), Pintea, S. (author), Hildebrandt, K.A. (author), Eisemann, E. (author), van Gemert, J.C. (author)Deep learning has improved vanishing point detection in images. Yet, deep networks require expensive annotated datasets trained on costly hardware and do not generalize to even slightly different domains, and minor problem variants. Here, we address these issues by injecting deep vanishing point detection networks with prior knowledge. This...conference paper 2022
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Tomas Martinez, S. (author), Chen, L.M. (author), Neu, Thomas R. (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Sialic acids are a family of nine-carbon negatively charged carbohydrates. In animals, they are abundant on mucosa surfaces as terminal carbohydrates of mucin glycoproteins. Some commensal and pathogenic bacteria are able to release, take up and catabolize sialic acids. Recently, sialic acids have been discovered to be widespread among most...journal article 2022
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Kim, Nam Kyeun (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Eco-friendly flame retardants are greatly required to meet the expectations of low-toxicity, environmental compatibility and sustainability. Extracellular polymeric substances (EPS), the biopolymers recovered from excess granular wastewater sludge, have been successfully incorporated into poly(vinyl alcohol) (PVA) by a solution casting method...journal article 2022
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Lin, Y. (author), Pintea, S. (author), van Gemert, J.C. (author)Current work on lane detection relies on large manually annotated datasets. We reduce the dependency on annotations by leveraging massive cheaply available unlabelled data. We propose a novel loss function exploiting geometric knowledge of lanes in Hough space, where a lane can be identified as a local maximum. By splitting lanes into separate...conference paper 2021
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Tomas Martinez, S. (author), Kleikamp, H.B.C. (author), Neu, Thomas R. (author), Pabst, Martin (author), Weissbrodt, D.G. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Nonulosonic acids (NulOs) are a family of acidic carbohydrates with a nine-carbon backbone, which include different related structures, such as sialic acids. They have mainly been studied for their relevance in animal cells and pathogenic bacteria. Recently, sialic acids have been discovered as an important compound in the extracellular...journal article 2021
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Kleikamp, H.B.C. (author), Lin, Y. (author), McMillan, D.G.G. (author), Geelhoed, Jeanine S. (author), Naus-Wiezer, Suzanne N.H. (author), Van Baarlen, Peter (author), Saha, Chinmoy (author), Louwen, Rogier (author), Sorokin, Dimitry Y. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)Nonulosonic acids, commonly referred to as sialic acids, are a highly important group of nine-carbon sugars common to all domains of life. They all share biosynthetic and structural features, but otherwise display a remarkable chemical diversity. In humans, sialic acids cover all cells which makes them important for processes such as cellular...review 2020
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Felz, S. (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Glycosaminoglycans (GAGs) are linear heteropolysaccharides containing a derivative of an amino sugar. The possibility of the presence of GAGs in aerobic granular sludge was studied by combining SDS-PAGE with Alcian Blue staining (at pH 2.5 and 1), FTIR, mammalian Hyaluronic acid and sulfated GAG analysis kits, enzymatic digestion and specific...journal article 2020
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Kim, Nam Kyeun (author), Mao, Ningtao (author), Lin, Richard (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)In this research, extracellular polymeric substances (EPS), such as EPS<sub>flocs</sub> and EPS<sub>granules</sub>, were successfully extracted from activated and aerobic granular sludge, respectively, and tested as bio-based flame retardant materials. Flax fabric was coated by the biopolymeric substances and its flammability was evaluated...journal article 2020
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Boleij, M. (author), Kleikamp, H.B.C. (author), Pabst, Martin (author), Neu, Thomas R. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Anammox (anaerobic ammonium oxidation) bacteria are important for the nitrogen cycle in both natural environments and wastewater treatment plants. These bacteria have a strong tendency to grow in aggregates like biofilms and granular sludge. To understand the formation of anammox aggregates, it is required to unravel the composition of the...journal article 2020
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Felz, S. (author), Kleikamp, H.B.C. (author), Zlopasa, J. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Structural extracellular polymeric substances (structural EPS) can form stable hydrogels and are considered to be responsible for the stability of biofilms and aerobic granular sludge. Structural EPS were extracted from aerobic granular sludge and characterized for their gel-forming capacity with different alkaline earth and transition metal...journal article 2020
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van Vliet, Daan M. (author), Lin, Y. (author), Bale, Nicole J. (author), Koenen, Michel (author), Villanueva, Laura (author), Stams, Alfons J.M. (author), Sánchez-Andrea, Irene (author)Recently, we isolated two marine strains, F1<sup>T</sup> and F21<sup>T</sup>, which together with Kiritimatiella glycovorans L21-Fru-AB<sup>T</sup> are the only pure cultures of the class Kiritimatiellae within the phylum Verrucomicrobiota. Here, we present an in-depth genome-guided characterization of both isolates with emphasis on their...journal article 2020
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