Searched for: author:"Mahfouz, A.M.E.T.A."
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Joglekar, Anoushka (author), Prjibelski, Andreyi (author), Mahfouz, A.M.E.T.A. (author), Collier, Paul (author), Lin, Susan (author), Schlusche, Anna Katharina (author), Marrocco, Jordan (author), Williams, Stephen R. (author), Haase, Bettina (author)
Splicing varies across brain regions, but the single-cell resolution of regional variation is unclear. We present a single-cell investigation of differential isoform expression (DIE) between brain regions using single-cell long-read sequencing in mouse hippocampus and prefrontal cortex in 45 cell types at postnatal day 7 (www.isoformAtlas.com...
journal article 2021
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Meijer, Mandy (author), Keo, D.L. (author), van Leeuwen, Judith M.C. (author), Dzyubachyk, Oleh (author), Meijer, Onno C. (author), Vinkers, Christiaan H. (author), Mahfouz, A.M.E.T.A. (author)
The biological mechanisms underlying inter-individual differences in human stress reactivity remain poorly understood. We aimed to identify the molecular underpinning of aberrant neural stress sensitivity in individuals at risk for schizophrenia. Linking mRNA expression data from the Allen Human Brain Atlas to task-based fMRI revealed 201...
journal article 2021
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van der Wijst, Mgp (author), de Vries, D. H. (author), Groot, H. E. (author), Trynka, G. (author), Hon, C. C. (author), Nawijn, M. C. (author), Ye, C. J. (author), Mahfouz, A.M.E.T.A. (author), Franke, L. (author)
In recent years, functional genomics approaches combining genetic information with bulk RNA-sequencing data have identified the downstream expression effects of disease-associated genetic risk factors through so-called expression quantitative trait locus (eQTL) analysis. Single-cell RNA-sequencing creates enormous opportunities for mapping...
journal article 2020
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Abdelaal, T.R.M. (author), Mourragui, S.M.C. (author), Mahfouz, A.M.E.T.A. (author), Reinders, M.J.T. (author)
Single-cell technologies are emerging fast due to their ability to unravel the heterogeneity of biological systems. While scRNA-seq is a powerful tool that measures whole-transcriptome expression of single cells, it lacks their spatial localization. Novel spatial transcriptomics methods do retain cells spatial information but some methods can...
journal article 2020
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Keo, D.L. (author), Mahfouz, A.M.E.T.A. (author), Ingrassia, Angela M.T. (author), Meneboo, Jean Pascal (author), Villenet, Celine (author), Mutez, Eugénie (author), Comptdaer, Thomas (author), Lelieveldt, B.P.F. (author), Reinders, M.J.T. (author)
The molecular mechanisms underlying caudal-to-rostral progression of Lewy body pathology in Parkinson’s disease remain poorly understood. Here, we identified transcriptomic signatures across brain regions involved in Braak Lewy body stages in non-neurological adults from the Allen Human Brain Atlas. Among the genes that are indicative of...
journal article 2020
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Salazar, A.N. (author), Luzia de Nobrega, F. (author), Anyansi, C.A. (author), Aparicio Maldonado, C. (author), Martins Costa, A.R. (author), van Eijkeren-Haagsma, A.C. (author), Hiralal, A.A. (author), Mahfouz, A.M.E.T.A. (author), McKenzie, R.E. (author), van Rossum, T. (author), Brouns, S.J.J. (author), Abeel, T.E.P.M.F. (author)
The last decade has witnessed a remarkable increase in our ability to measure genetic information. Advancements of sequencing technologies are challenging the existing methods of data storage and analysis. While methods to cope with the data deluge are progressing, many biologists have lagged behind due to the fast pace of computational...
journal article 2020
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de Vries, Natasja L. (author), Mahfouz, A.M.E.T.A. (author), Koning, Frits (author), de Miranda, Noel F.C.C. (author)
Cancers are characterized by extensive heterogeneity that occurs intratumorally, between lesions, and across patients. To study cancer as a complex biological system, multidimensional analyses of the tumor microenvironment are paramount. Single-cell technologies such as flow cytometry, mass cytometry, or single-cell RNA-sequencing have...
review 2020
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Abdelaal, T.R.M. (author), van Unen, Vincent (author), Höllt, T. (author), Koning, Frits (author), Reinders, M.J.T. (author), Mahfouz, A.M.E.T.A. (author)
Mass cytometry by time-of-flight (CyTOF) is a valuable technology for high-dimensional analysis at the single cell level. Identification of different cell populations is an important task during the data analysis. Many clustering tools can perform this task, which is essential to identify “new” cell populations in explorative experiments....
journal article 2019
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Ziats, Catherine A. (author), Grosvenor, Luke P. (author), Sarasua, Sara M. (author), Thurm, Audrey E. (author), Swedo, Susan E. (author), Mahfouz, A.M.E.T.A. (author), Rennert, Owen M. (author), Ziats, Mark N. (author)
Phelan-McDermid syndrome (PMS) is a neurodevelopmental disorder characterized by varying degrees of intellectual disability, severely delayed language development and specific facial features, and is caused by a deletion within chromosome 22q13.3. SHANK3, which is located at the terminal end of this region, has been repeatedly implicated in...
journal article 2019
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Viho, Eva M.G. (author), Buurstede, Jacobus C. (author), Mahfouz, A.M.E.T.A. (author), Koorneef, Lisa L. (author), Van Weert, Lisa T.C.M. (author), Houtman, René (author), Hunt, Hazel J. (author), Kroon, Jan (author), Meijer, Onno C. (author)
Glucocorticoid hormones have important effects on brain function in the context of acute and chronic stress. Many of these are mediated by the glucocorticoid receptor (GR). GR has transcriptional activity which is highly context-specific and differs between tissues and even between cell types. The outcome of GR-mediated transcription depends...
journal article 2019
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de Vries, N.L. (author), Van Unen, Vincent (author), Ijsselsteijn, Marieke E. (author), Abdelaal, T.R.M. (author), Van Der Breggen, Ruud (author), Farina Sarasqueta, Arantza (author), Mahfouz, A.M.E.T.A. (author), Peeters, Koen C.M.J. (author), Höllt, T. (author), Lelieveldt, B.P.F. (author), Koning, Frits (author), De Miranda, Noel F.C.C. (author)
Objective: A comprehensive understanding of anticancer immune responses is paramount for the optimal application and development of cancer immunotherapies. We unravelled local and systemic immune profiles in patients with colorectal cancer (CRC) by high-dimensional analysis to provide an unbiased characterisation of the immune contexture of...
journal article 2019
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Abdelaal, T.R.M. (author), Höllt, T. (author), van Unen, Vincent (author), Lelieveldt, B.P.F. (author), Koning, Frits (author), Reinders, M.J.T. (author), Mahfouz, A.M.E.T.A. (author)
Motivation: High-dimensional mass cytometry (CyTOF) allows the simultaneous measurement of multiple cellular markers at single-cell level, providing a comprehensive view of cell compositions.<br/>However, the power of CyTOF to explore the full heterogeneity of a biological sample at the singlecell level is currently limited by the number of...
journal article 2019
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Abdelaal, T.R.M. (author), Michielsen, L.C.M. (author), Cats, Davy (author), Hoogduin, Dylan (author), Mei, Hailiang (author), Reinders, M.J.T. (author), Mahfouz, A.M.E.T.A. (author)
Background: Single-cell transcriptomics is rapidly advancing our understanding of the cellular composition of complex tissues and organisms. A major limitation in most analysis pipelines is the reliance on manual annotations to determine cell identities, which are time-consuming and irreproducible. The exponential growth in the number of...
journal article 2019
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Koorneef, Lisa L. (author), Bogaards, Marit (author), Reinders, M.J.T. (author), Meijer, Onno C. (author), Mahfouz, A.M.E.T.A. (author)
Metabolic status impacts on the emotional brain to induce behavior that maintains energy balance. While hunger suppresses the fear circuitry to promote explorative food-seeking behavior, satiety or obesity may increase fear to prevent unnecessary risk-taking. Here we aimed to unravel which metabolic factors, that transfer information about...
journal article 2018
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Mahinrad, Simin (author), Bulk, Marjolein (author), Van Der Velpen, Isabelle (author), Mahfouz, A.M.E.T.A. (author), Van Roon-Mom, Willeke (author), Fedarko, Neal (author), Yasar, Sevil (author), Sabayan, Behnam (author), Van Heemst, Diana (author), Van Der Weerd, Louise (author)
Animal studies suggest the involvement of natriuretic peptides (NP) in several brain functions that are known to be disturbed during Alzheimer's disease (AD). However, it remains unclear whether such findings extend to humans. In this study, we aimed to: (1) map the gene expression and localization of NP and their receptors (NPR) in human...
journal article 2018
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Coutinho de Almeida, Rodrigo (author), Ramos, Yolande F.M. (author), Mahfouz, A.M.E.T.A. (author), den Hollander, Wouter (author), Lakenberg, Nico (author), Houtman, Evelyn (author), van Hoolwerff, Marcella (author), Suchiman, H. Eka D. (author), Rodriguez-Ruiz, Alejandro (author), Slagboom, P. Eline (author), Mei, Hailiang (author), Kielbasa, Szymon M. (author), Nelissen, R.G.H.H. (author), Reinders, M.J.T. (author), Meulenbelt, Ingrid (author)
Objective To uncover the microRNA (miRNA) interactome of the osteoarthritis (OA) pathophysiological process in the cartilage.<br/>Methods We performed RNA sequencing in 130 samples (n=35 and n=30 pairs for messenger RNA (mRNA) and miRNA, respectively) on macroscopically preserved and lesioned OA cartilage from the same patient and performed...
journal article 2018
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Keo, D.L. (author), Aziz, N. Ahmad (author), Dzyubachyk, Oleh (author), Van Der Grond, Jeroen (author), van Roon-Mom, Willeke M.C. (author), Lelieveldt, B.P.F. (author), Reinders, M.J.T. (author), Mahfouz, A.M.E.T.A. (author)
Cytosine-adenine-guanine (CAG) repeat expansions in the coding regions of nine polyglutamine (polyQ) genes (HTT, ATXN1, ATXN2, ATXN3, CACNA1A, ATXN7, ATN1, AR, and TBP) are the cause of several neurodegenerative diseases including Huntington’s disease (HD), six different spinocerebellar ataxias (SCAs), dentatorubral-pallidoluysian atrophy, and...
journal article 2017
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Doorenweerd, Nathalie (author), Mahfouz, A.M.E.T.A. (author), van Putten, Maaike (author), Kaliyaperumal, Rajaram (author), 't Hoen, Peter A.C. (author), Hendriksen, Jos G.M. (author), Aartsma-Rus, Annemieke M. (author), Verschuuren, Jan J.G.M. (author), Niks, Erik H. (author), Reinders, M.J.T. (author), Kan, Hermien E. (author), Lelieveldt, B.P.F. (author)
Duchenne muscular dystrophy (DMD) is a muscular dystrophy with high incidence of learning and behavioural problems and is associated with neurodevelopmental disorders. To gain more insights into the role of dystrophin in this cognitive phenotype, we performed a comprehensive analysis of the expression patterns of dystrophin isoforms across...
journal article 2017
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Eising, Else (author), Shyti, Reinald (author), 't Hoen, Peter A.C. (author), Vijfhuizen, L.S. (author), Huisman, S.M.H. (author), Broos, Ludo A. M. (author), Mahfouz, A.M.E.T.A. (author), Reinders, M.J.T. (author), Ferrari, Michel D. (author), Tolner, Else A. (author), de Vries, Boukje (author), van den Maagdenberg, Arn M.J.M. (author)
Familial hemiplegic migraine type 1 (FHM1) is a rare monogenic subtype of migraine with aura caused by mutations in CACNA1A that encodes the α1A subunit of voltage-gated CaV2.1 calcium channels. Transgenic knock-in mice that carry the human FHM1 R192Q missense mutation (‘FHM1 R192Q mice’) exhibit an increased susceptibility to cortical spreading...
journal article 2017
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Mahfouz, A.M.E.T.A. (author), Huisman, S.M.H. (author), Lelieveldt, B.P.F. (author), Reinders, M.J.T. (author)
The immense complexity of the mammalian brain is largely reflected in the underlying molecular signatures of its billions of cells. Brain transcriptome atlases provide valuable insights into gene expression patterns across different brain areas throughout the course of development. Such atlases allow researchers to probe the molecular mechanisms...
journal article 2017
Searched for: author:"Mahfouz, A.M.E.T.A."
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