Microsecond time-scale kinetics of transient biochemical reactions

To afford mechanistic studies in enzyme kinetics and protein folding in the microsecond time domain we have developed a continuous-flow microsecond time-scale mixing instrument with an unprecedented dead-time of 3.8 ± 0.3 μs. The instrument employs a micro-mixer with a mixing time of 2.7 μs integrated with a 30 mm long flow-cell of 109 μm...

Ultrafast tangential micro-mixers for the study of biochemical reactions on the microsecond time scale

Detailed understanding of chemical and enzyme catalysis constitutes a main focus of current biochemical research. Fundamental insight in how (bio)catalysts function, requires knowledge of their three dimensional structure and a wide range of time resolved experiments that monitor the reaction progress. The ultimate aim is the determination of...