Searched for: author:"Pozzi, P."
(1 - 13 of 13)
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Pozzi, P. (author), Maddalena, L. (author), Ceffa, N.G. (author), Soloviev, O.A. (author), Vdovin, Gleb (author), Carroll, E.C.M. (author), Verhaegen, M.H.G. (author)
The use of spatial light modulators to project computer generated holograms is a common strategy for optogenetic stimulation of multiple structures of interest within a three-dimensional volume. A common requirement when addressing multiple targets sparsely distributed in three dimensions is the generation of a points cloud, focusing excitation...
journal article 2019
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Pozzi, P. (author), Soloviev, O.A. (author), Wilding, D. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
We report on a universal sample-independent sensorless adaptive optics method, based on modal optimization of the second moment of the fluorescence emission from a point-like excitation. Our method employs a sample-independent precalibration, performed only once for the particular system, to establish the direct relation between the image...
journal article 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
Three-dimensional microscopy suffers from sample-induced aberrations that reduce the resolution and lead to misinterpretations of the object distribution. In this paper, the resolution of a three-dimensional fluorescent microscope is significantly improved by introducing an amplitude diversity in the form of a binary amplitude mask positioned...
journal article 2018
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
In this work, we present a new confocal laser scanning microscope capable to perform sensorless wavefront optimization in real time. The device is a parallelized laser scanning microscope in which the excitation light is structured in a lattice of spots by a spatial light modulator, while a deformable mirror provides aberration correction and...
conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Fiolka, Reto (author), Verhaegen, M.H.G. (author)
The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...
conference paper 2018
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
In life sciences, interest in the microscopic imaging of increasingly complex three dimensional samples, such as cell spheroids, zebrafish embryos, and in vivo applications in small animals, is growing quickly. Due to the increasing complexity of samples, more and more life scientists are considering the implementation of adaptive optics in...
conference paper 2018
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Wilding, D. (author), Soloviev, O.A. (author), Pozzi, P. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
A methodology for retrieving the unknown object distribution and point-spread functions (PSFs) from a set of images acquired in the presence of temporal phase aberrations is presented in this paper. The method works by finding optimal complimentary linear filters for multi-frame deconvolution. The algorithm uses undemanding computational...
journal article 2017
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Wilding, D. (author), de Iongh, Gijs (author), Soloviev, O.A. (author), Pozzi, P. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
By taking multiple input-output measurements, it is shown how to determine the input to an optical system that corrects unknown phase aberrations without interferometric measurements or online iterative optimization within a couple of seconds. It is shown to work in simulations and experiment. This technique may also be used to acquire the...
conference paper 2017
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Pozzi, P. (author), Wilding, D. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
This proceeding reports early results in the development of a new technique for adaptive optics in confocal microscopy. The term adaptive optics refers to the branch of optics in which an active element in the optical system is used to correct inhomogeneities in the media through which light propagates. In its most classical form, mostly used in...
conference paper 2016
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Wilding, D. (author), Pozzi, P. (author), Soloviev, O.A. (author), Vdovine, G.V. (author), Verhaegen, M.H.G. (author)
Aberrations, scattering and absorption degrade the performance light-sheet fluorescence microscopes (LSFM). An adaptive optics system to correct for these artefacts and to optimize the light-sheet illumination is presented. This system allows a higher axial resolution to be recovered over the field-of-view of the detection objective. It is...
conference paper 2016
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Gong, H. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovine, G.V. (author)
We present a reference-less and time-multiplexing phase retrieval method by making use of the digital micromirror device (DMD). In this method, the DMD functions not only as a flexible binary mask which modulates the optical field, but also as a sampling mask for measuring corresponding phases, which makes the whole setup simple and robust. The...
conference paper 2016
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Gong, H. (author), Pozzi, P. (author), Soloviev, O.A. (author), Verhaegen, M.H.G. (author), Vdovine, G.V. (author)
We investigate the possibility of interferometric optical field sensor with DMD forming a series of sampling interferometers in the pupil of an optical system. Our preliminary experiment validates the feasibility of our approach for the field reconstruction in the case of coherent field. We believe that this approach can be extended to sensing...
conference paper 2015
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Vdovine, G.V. (author), Gong, H. (author), Soloviev, O.A. (author), Pozzi, P. (author), Verhaegen, M.H.G. (author)
We have experimentally demonstrated a lensless coherent microscope based on direct registration of the complex optical field by sampling the pupil with a sequence of two-point interferometers formed by a digital micromirror device. Complete registration of the complex amplitude in the pupil of the imaging system, without any reference beam,...
journal article 2015
Searched for: author:"Pozzi, P."
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