Analysis of centrifugal homogenization and its applications for emulsification & mechanical cell lysis

We detail the analysis of centrifugal homogenization process by a hydrodynamic model and the model-guided design of a low-cost centrifugal homogenizer. During operation, centrifugal force pushes a multiphase solution to be homogenized through a thin nozzle, consequently homogenizing its contents. We demonstrate and assess the homogenization...

Aquaporin-2 trafficking: Studying cellular mechanisms with subcellular aspiration and cryo-electron microscopy

SMA-SH: Modified styrene maleic acid copolymer for functionalization of lipid nanodiscs

Challenges in purification and subsequent functionalization of membrane proteins often complicate their biochemical and biophysical characterization. Purification of membrane proteins generally involves replacing the lipids surrounding the protein with detergent molecules, which can affect protein structure and function. Recently, it was shown...