Searched for: author%3A%22Rieger%2C+B.%22
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Cheishvili, K. (author), Rieger, B. (author), Kalkman, J. (author)
We quantify the precision and bias of dynamic light scattering optical coherence tomography (DLS-OCT) measurements of the diffusion coefficient and flow speed for first and second-order normalized autocovariance functions. For both diffusion and flow, the measurement precision and accuracy are severely limited by correlations between the...
journal article 2024
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van Velde, P.F. (author), Rieger, B. (author), Hinsdale, T.A. (author), Cnossen, J.P. (author), Fan, D. (author), Hung, S. (author), Grunwald, D. (author), Smith, C.S. (author)
Three dimensional modulation-enhanced single-molecule localization techniques, such as ModLoc, offer advancements in axial localization precision across the entire field of view and axial capture range, by applying phase shifting to the illumination pattern. However, this improvement is limited by the pitch of the illumination pattern that...
journal article 2023
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Haghparast, S. (author), Stallinga, S. (author), Rieger, B. (author)
Fusion of multiple chemically identical complexes, so-called particles, in localization microscopy, can improve the signal-to-noise ratio and overcome under-labeling. To this end, structural homogeneity of the data must be assumed. Biological heterogeneity, however, could be present in the data originating from distinct conformational...
journal article 2023
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Wang, W. (author), Jakobi, A. (author), Wu, Yu-Le (author), Ries, Jonas (author), Stallinga, S. (author), Rieger, B. (author)
Single molecule localization microscopy offers resolution nearly down to the molecular level with specific molecular labelling, and is thereby a promising tool for structural biology. In practice, however, the actual value to this field is limited primarily by incomplete fluorescent labelling of the structure. This missing information can be...
journal article 2023
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Geleijnse, G. (author), Veder, L. L. (author), Hakkesteegt, M. M. (author), de Gier, H. H.W. (author), Rieger, B. (author), Metselaar, R. M. (author)
Objectives: Digital endoscopes are connected to a video processor that applies various operations to process the image. One of those operations is edge enhancement that sharpens the image. The purpose of this study was to (1) quantify the level of edge enhancement, (2) measure the effect on sharpness and image noise, and (3) study the...
journal article 2023
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Wang, W. (author), Jakobi, A. (author), Wu, Yu‑Le ‑L (author), Ries, Jonas (author), Stallinga, S. (author), Rieger, B. (author)
Correction to: Scientific Reports, published online 16 August 2023 The original version of this Article contained an error in the upper inset of Figure 4, where the atomic model was missing. The original Figure 4 and accompanying legend appear below. (Figure presented.) Overlay of the fluorophore positions from the SMLM particle fusion data ...
journal article 2023
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Geleijnse, Geert (author), Rieger, B. (author)
[This corrects the article DOI: 10.1117/1.JBO.27.10.106001.].
journal article 2023
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Fazel, Mohamadreza (author), Wester, Michael J. (author), Schodt, David J. (author), Cruz, Sebastian Restrepo (author), Strauss, Sebastian (author), Schueder, Florian (author), Schlichthaerle, Thomas (author), Lidke, Keith A. (author), Rieger, B. (author)
Single-molecule localization microscopy super-resolution methods rely on stochastic blinking/binding events, which often occur multiple times from each emitter over the course of data acquisition. Typically, the blinking/binding events from each emitter are treated as independent events, without an attempt to assign them to a particular...
journal article 2022
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Thorsen, R.Ø. (author), Hulleman, C.N. (author), Rieger, B. (author), Stallinga, S. (author)
Combining orientation estimation with localization microscopy opens up the possibility to analyze the underlying orientation of biomolecules on the nanometer scale. Inspired by the recent improvement of the localization precision by shifting excitation patterns (MINFLUX, SIMFLUX), we have adapted the idea towards the modulation of excitation...
journal article 2022
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Geleijnse, Geert (author), Rieger, B. (author)
SignificanceFlexible endoscopes are essential for medical internal examinations. Digital endoscopes are connected to a video processor that can apply various operations to enhance the image. One of those operations is edge enhancement, which has a major impact on the perceived image quality by medical professionals. However, the specific...
journal article 2022
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Wang, W. (author), Heydarian, H. (author), Huijben, T.A.P.M. (author), Stallinga, S. (author), Rieger, B. (author)
Summary: We present a fast particle fusion method for particles imaged with single-molecule localization microscopy. The state-of-the-art approach based on all-to-all registration has proven to work well but its computational cost scales unfavorably with the number of particles N, namely as N2. Our method overcomes this problem and achieves a...
journal article 2022
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Hinsdale, T.A. (author), Stallinga, S. (author), Rieger, B. (author)
Structured illumination microscopy (SIM) is a widely used imaging technique that doubles the effective resolution of widefield microscopes. Most current implementations rely on diffractive elements, either gratings or programmable devices, to generate structured light patterns in the sample. These can be limited by spectral efficiency, speed, or...
journal article 2021
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Hulleman, C.N. (author), Thorsen, R.Ø. (author), Kim, E. (author), Dekker, C. (author), Stallinga, S. (author), Rieger, B. (author)
Estimating the orientation and 3D position of rotationally constrained emitters with localization microscopy typically requires polarization splitting or a large engineered Point Spread Function (PSF). Here we utilize a compact modified PSF for single molecule emitter imaging to estimate simultaneously the 3D position, dipole orientation, and...
journal article 2021
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Huijben, T.A.P.M. (author), Heydarian, H. (author), Auer, Alexander (author), Schueder, Florian (author), Jungmann, Ralf (author), Stallinga, S. (author), Rieger, B. (author)
Particle fusion for single molecule localization microscopy improves signal-to-noise ratio and overcomes underlabeling, but ignores structural heterogeneity or conformational variability. We present a-priori knowledge-free unsupervised classification of structurally different particles employing the Bhattacharya cost function as dissimilarity...
journal article 2021
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Heydarian, H. (author), Joosten, M.J. (author), Przybylski, A. (author), Schueder, Florian (author), Jungmann, Ralf (author), van Werkhoven, B.J.C. (author), Keller-Fiendeisen, J. (author), Rieger, B. (author), Stallinga, S. (author)
Single molecule localization microscopy offers in principle resolution down to the molecular level, but in practice this is limited primarily by incomplete fluorescent labeling of the structure. This missing information can be completed by merging information from many structurally identical particles. In this work, we present an approach for 3D...
journal article 2021
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Hulleman, C.N. (author), Moerland, R.J. (author), Stallinga, S. (author), Rieger, B. (author)
With the growing popularity of cryogenic correlative light and electron microscopy, it is becoming increasingly important to bridge the resolution gap between these two modalities. At cryogenic temperatures, the photon yield of fluorophores is a few orders of magnitude higher than at room temperature, enabling localization precisions on the...
journal article 2021
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Li, Q. (author), Hulleman, C.N. (author), Moerland, R.J. (author), Mailvaganam, Elil (author), Ganapathy, S. (author), Brinks, D. (author), Stallinga, S. (author), Rieger, B. (author)
Total internal reflection fluorescence (TIRF) microscopy is an important imaging tool for the investigation of biological structures, especially the study on cellular events near the plasma membrane. Imaging at cryogenic temperatures not only enables observing structures in a near-native and fixed state but also suppresses irreversible photo...
journal article 2021
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Janssen, G.C.A.M. (author), van der Pers, N.M. (author), Hendrikx, R.W.A. (author), Bottger, A.J. (author), Kwakernaak, C. (author), Rieger, B. (author), Sluiter, M.H.F. (author)
Single-crystal copper films on sapphire have recently been reported upon in relation to graphene growth on these films. In the present paper the kinetics of the formation of single crystal copper films is investigated. We demonstrate the importance of heating the sapphire substrate in 1000 hPa oxygen, followed by a fast cooling prior to...
journal article 2020
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Cnossen, J.P. (author), Hinsdale, T.A. (author), Thorsen, R.Ø. (author), Siemons, Marijn (author), Schueder, Florian (author), Jungmann, Ralf (author), Smith, C.S. (author), Rieger, B. (author), Stallinga, S. (author)
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold...
journal article 2020
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Guo, Y. (author), Rieger, B. (author)
Regularization has been introduced to electron tomography for enhancing the reconstruction quality. Since over-regularization smears out sharp edges and under-regularization leaves the image too noisy, finding the optimal regularization strength is crucial. To this end, one can either manually tune regularization parameters by trial and error...
conference paper 2019
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