Searched for: author:"Stallinga, S."
(1 - 20 of 36)

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Ambrosini, P. (author), Hollemans, Eva (author), Kweldam, Charlotte F. (author), Leenders, Geert J.L.H.van (author), Stallinga, S. (author), Vos, F.M. (author)
Cribriform growth patterns in prostate carcinoma are associated with poor prognosis. We aimed to introduce a deep learning method to detect such patterns automatically. To do so, convolutional neural network was trained to detect cribriform growth patterns on 128 prostate needle biopsies. Ensemble learning taking into account other tumor...
journal article 2020
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Kner, Peter (author), Manley, Suliana (author), Shechtman, Yoav (author), Stallinga, S. (author)
This feature issue commemorating 25 years of STED microscopy and 20 years of SIM is intended to highlight the incredible progress and growth in the field of superresolution microscopy since Stefan Hell and Jan Wichmann published the article Breaking the diffraction resolution limit by stimulated emission: stimulated-emission-depletion...
journal article 2020
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Cnossen, J.P. (author), Hinsdale, T.A. (author), Thorsen, R.Ø. (author), Siemons, Marijn (author), Schueder, Florian (author), Jungmann, Ralf (author), Smith, C.S. (author), Rieger, B. (author), Stallinga, S. (author)
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in field of view. Here we combine centroid estimation and illumination pattern induced photon count variations in a conventional widefield imaging setup to extract position information over a typical micrometer-sized field of view. We show a near two-fold...
journal article 2020
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van der Graaff, L. (author), Van Leenders, Geert J.L.H. (author), Boyaval, Fanny (author), Stallinga, S. (author)
Whole-slide imaging systems can generate full-color image data of tissue slides efficiently, which are needed for digital pathology applications. This paper focuses on a scanner architecture that is based on a multi-line image sensor that is tilted with respect to the optical axis, such that every line of the sensor scans the tissue slide at...
journal article 2020
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Berlich, R. (author), Stallinga, S. (author)
A practical method for determining wavefront aberrations in optical systems based on the acquisition of an extended, unknown object is presented. The approach utilizes a conventional phase diversity approach in combination with a pupil-engineered, helical point spread function (PSF) to discriminate the aberrated PSF from the object features....
journal article 2020
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Kragt, Augustinus J.J. (author), Hoekstra, Davey C. (author), Stallinga, S. (author), Broer, Dirk J. (author), Schenning, Albertus P.H.J. (author)
Engineering the helical structure of chiral photonic materials in three dimensions remains a challenge. 3D helix engineered photonic materials are fabricated by local stratification in a photopolymerizable chiral nematic liquid crystal. The obtained chiral photonic materials reflect both handedness of circular polarized light and show super...
journal article 2019
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van der Graaff, L. (author), van Leenders, Geert J. L. H. (author), Boyaval, Fanny (author), Stallinga, S. (author)
Confocal scanning microscopy is the de facto standard modality for fluorescence imaging. Point scanning, however, leads to a limited throughput and makes the technique unsuitable for fast multi-focal scanning over large areas. We propose an architecture for multi-focal fluorescence imaging that is scalable to large area imaging. The design is...
journal article 2019
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van der Graaff, L. (author), Stallinga, S. (author)
Confocal fluorescent imaging is the de facto standard modality for fluorescence imaging. However, the point-to-point scanning technique leads to a very limited throughput and makes the technique unsuitable for large area and fast multi-focal scanning. We propose an architecture for highly efficient 3D line confocal fluorescence imaging. Our...
conference paper 2019
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Siemons, M.E. (author), Hulleman, C.N. (author), Thorsen, R.Ø. (author), Smith, C.S. (author), Stallinga, S. (author)
Point spread function (PSF) engineering is used in single emitter localization to measure the emitter position in 3D and possibly other parameters such as the emission color or dipole orientation as well. Advanced PSF models such as spline fits to experimental PSFs or the vectorial PSF model can be used in the corresponding localization...
journal article 2018
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Thorsen, R.Ø. (author), Hulleman, Christiaan N. (author), Hammer, Mathias (author), Grünwald, David (author), Stallinga, S. (author), Rieger, B. (author)
journal article 2018
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Berlich, Ren (author), Stallinga, S. (author)
An approach for designing purely refractive optical elements that generate engineered, multi-order-helix point spread functions (PSFs) with large peak separation for passive, optical depth measurement is presented. The influence of aberrations on the PSF’s rotation angle, which limits the depth retrieval accuracy, is studied numerically and...
journal article 2018
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Hulleman, C.N. (author), Huisman, Maximiliaan (author), Moerland, R.J. (author), Gruenwald, David (author), Stallinga, S. (author), Rieger, B. (author)
Light microscopy, allowing sub-diffraction-limited resolution, has been among the fastest developing techniques at the interface of biology, chemistry, and physics. Intriguingly no theoretical limit exists on how far the underlying measurement uncertainty can be lowered. In particular data fusion of large amounts of images can reduce the...
journal article 2018
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Heydarian, H. (author), Schueder, Florian (author), Strauss, Maximilian T. (author), van Werkhoven, Ben (author), Fazel, Mohamadreza (author), Lidke, K.A. (author), Jungmann, Ralf (author), Stallinga, S. (author), Rieger, B. (author)
Methods that fuse multiple localization microscopy images of a single structure can improve signal-to-noise ratio and resolution, but they generally suffer from template bias or sensitivity to registration errors. We present a template-free particle-fusion approach based on an all-to-all registration that provides robustness against...
journal article 2018
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Hulleman, C.N. (author), Huisman, Maximiliaan (author), Moerland, R.J. (author), Grünwald, David (author), Stallinga, S. (author), Rieger, B. (author)
In article number 1700323, a sparsity‐inducing scheme based on widefield stimulated emission depletion, which requires stringent control of the polarization state of both the excitation and depletion laser, is introduced by Bernd Rieger and co‐workers for superresolution fluorescence microscopy. The ideal state involves excitation and depletion...
journal article 2018
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van der Graaff, L. (author), Boyaval, Fanny (author), van Vliet, L.J. (author), Stallinga, S. (author)
In the field of pathology there is an ongoing transition to the use of Whole Slide Imaging (WSI) systems which scan tissue slides at intermediate resolution (0∼.25 μm) and high throughput (15mm<sup>2</sup>=min) to digital image files. Most scanners currently on the market are line-sensor based push-broom scanners for three-color (RGB)...
conference paper 2018
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Shakeri, S.M. (author), van Vliet, L.J. (author), Stallinga, S. (author)
We present an investigation of the impact of partial coherence on optical imaging systems with the focus on whole slide imaging (WSI) systems for digital pathology. The investigation is based on the analysis of the edge response of the optical system, which gives rise to an apparent optical transfer function (OTF) that can be linked to two...
journal article 2017
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Rieger, B. (author), Stallinga, S. (author)
Standard fluorescent light microscopy is rapidly approaching resolutions of a few nanometers when computationally combining information from hundreds of identical structures. We have developed algorithms to combine this information taking into account the specifics of fluorescent imaging.
journal article 2017
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Chakrova, N. (author), Rieger, B. (author), Stallinga, S. (author)
We present a versatile fluorescence microscope, built by complementing a conventional fluorescence microscope with a digital micro-mirror device (DMD) in the illumination path. Arbitrary patterns can be created on the DMD and projected onto the sample. This patterned illumination can be used to improve lateral and axial resolution over the...
conference paper 2015
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Stallinga, S. (author)
Localization microscopy for imaging at the nano-scale relies on the quality of fitting the emitter positions from the measured light spots. The type and magnitude of the noise in the detection process, the background light level and the Point Spread Function model that is used in the fit are of paramount importance for the precision and accuracy...
conference paper 2015
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Nieuwenhuizen, R.P.J. (author), Bates, M. (author), Szymborska, A. (author), Lidke, K.A. (author), Rieger, B. (author), Stallinga, S. (author)
journal article 2015
Searched for: author:"Stallinga, S."
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