Hybrid adaptive and computational light-sheet fluorescence microscopy

The light-sheet fluorescence microscopy is an excellent tool for the investigation of large three dimensional microscopy samples at the cellular level, however, the ability to resolve features is strongly affected by the presence of scattering and aberrations. These effects are two fold in light-sheet microscopy, as the illumination path...

Joint optimization of phase diversity and adaptive optics: Demonstration of potential

We study different possibilities to use adaptive optics (AO) and phase diversity (PD) together in a jointly optimized system. The potential of the joint system is demonstrated through numerical simulations. We find that the most significant benefits are obtained from the improved deconvolution of AO-corrected wavefronts and the additional...