Orientation-Locked DNA Origami for Stable Trapping of Small Proteins in the Nanopore Electro-Osmotic Trap

Nanopores are versatile single-molecule sensors offering a simple label-free readout with great sensitivity. We recently introduced the nanopore electro-osmotic trap (NEOtrap) which can trap and sense single unmodified proteins for long times. The trapping is achieved by the electro-osmotic flow (EOF) generated from a DNA-origami sphere...

A Generalized Transformer-Based Pulse Detection Algorithm

Pulse-like signals are ubiquitous in the field of single molecule analysis, e.g., electrical or optical pulses caused by analyte translocations in nanopores. The primary challenge in processing pulse-like signals is to capture the pulses in noisy backgrounds, but current methods are subjectively based on a user-defined threshold for pulse...

The time-varying nature of the asymmetrical flow of a shear-thinning polymer solution in transitional pipe flow

Previous studies of shear-thinning fluids in pipe flow discovered that, although the time-averaged velocity profile was axisymmetric when the flow was laminar or fully turbulent, contrary to expectations it was asymmetric in the laminar-turbulent transition regime. The general consensus of these previous experiments was that the location of the...