Searched for: author:"Wiktor, J.M."
(1 - 4 of 4)
document
Wu, Fabai (author), Japaridze, A. (author), Zheng, X.Z. (author), Wiktor, J.M. (author), Kerssemakers, J.W.J. (author), Dekker, C. (author)
Although the physical properties of chromosomes, including their morphology, mechanics, and dynamics are crucial for their biological function, many basic questions remain unresolved. Here we directly image the circular chromosome in live E. coli with a broadened cell shape. We find that it exhibits a torus topology with, on average, a lower...
journal article 2019
document
Wiktor, J.M. (author), van der Does, M. (author), Büller, L.A. (author), Sherratt, David J. (author), Dekker, C. (author)
The formation of 3 single-stranded DNA overhangs is a first and essential step during homology-directed repair of double-stranded breaks (DSB) of DNA, a task that in Escherichia coli is performed by RecBCD. While this protein complex has been well characterized through in vitro single-molecule studies, it has remained elusive how end...
journal article 2018
document
Wiktor, J.M. (author)
It seems that the evolution of life on this planet repeatedly acknowledges the value of<br/>the genetic information, by providing an abundant variety of elegant mechanisms for the repair of damage occurring to DNA. The need for such mechanisms tells us that the state of cellular DNA is under constant threat of disintegration and decay. This may...
doctoral thesis 2017
document
Wiktor, J.M. (author), Lesterlin, Christian (author), Sherratt, David J. (author), Dekker, C. (author)
Programmable control of the cell cycle has been shown to be a powerful tool in cell-biology studies. Here, we develop a novel system for controlling the bacterial cell cycle, based on binding of CRISPR/dCas9 to the origin-of-replication locus. Initiation of replication of bacterial chromosomes is accurately regulated by the DnaA protein,...
journal article 2016
Searched for: author:"Wiktor, J.M."
(1 - 4 of 4)