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De Vries, S. (author), Yildirim, E. (author)Production of chimeric polynucleotides by linking a double-stranded polynucleotide comprising a first gene or gene fragment at its 3' end to a double-stranded polynucleotide comprising a second gene or gene fragment at its 5' end comprises blunt-ending the two polynucleotides and ligating the blunt ends.patent 2004