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Martens, Koen (author), van Beljouw, Sam P.B. (author), van der Els, Simon (author), Vink, J.N.A. (author), Baas, Sander (author), Vogelaar, George A. (author), Brouns, S.J.J. (author), van Baarlen, Peter (author), Kleerebezem, Michiel (author), Hohlbein, Johannes (author)
CRISPR-Cas9 is widely used in genomic editing, but the kinetics of target search and its relation to the cellular concentration of Cas9 have remained elusive. Effective target search requires constant screening of the protospacer adjacent motif (PAM) and a 30 ms upper limit for screening was recently found. To further quantify the rapid...
journal article 2019