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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Cingoz, Seyma (author), Yoruk, Mustafa (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author), Ersahin, Mustafa Evren (author)Achieving a neutral/positive energy balance without compromising discharge standards is one of the main goals of wastewater treatment plants (WWTPs) in terms of sustainability. Aerobic granular sludge (AGS) technology promises high treatment performance with low energy and footprint requirement. In this study, high-rate activated sludge (HRAS...journal article 2023
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Páez Watson, T.W. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)The understanding of microbial communities and the biological regulation of its members is crucial for implementation of novel technologies using microbial ecology. One poorly understood metabolic principle of microbial communities is resource allocation and biosynthesis. Resource allocation theory in polyphosphate accumulating organisms ...journal article 2023
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Ochs, Pascal (author), Martin, Ben (author), Germain-Cripps, Eve (author), Stephenson, Tom (author), van Loosdrecht, Mark C.M. (author), Soares, Ana (author)Over the past twenty years, various commercial technologies have been deployed to remove ammonia (NH<sub>4</sub>–N) from anaerobic digestion (AD) liquors. In recent years many anaerobic digesters have been upgraded to include a pre-treatment, such as the thermal hydrolysis process (THP), to produce more biogas, increasing NH<sub>4</sub>–N...journal article 2023
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Ziliani, Agustina (author), Bovio-Winkler, Patricia (author), Cabezas, Angela (author), Etchebehere, Claudia (author), Garcia, Hector A. (author), López-Vázquez, Carlos M. (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Rubio-Rincón, Francisco J. (author)Ca. Accumulibacter was the predominant microorganism (relative FISH bio-abundance of 67 ± 5%) in a lab-scale sequential batch reactor that accomplished enhanced biological phosphorus removal (EBPR) while using glucose and acetate as the carbon sources (1:1 COD-based ratio). Both organic compounds were completely anaerobically consumed. The...journal article 2023
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Wang, Xiangyang (author), Shi, Chen (author), Hao, Xiaodi (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Wet-chemical approach is widely applied for phosphate recovery from incinerated ash of waste activated sludge (WAS), along with metals removed/recovered. The high contents of both aluminum (Al) and iron (Fe) in WAS-incinerated ash should be suitable for producing coagulants with some waste anions like Cl<sup>−</sup> and SO<sub>4</sub><sup>2−<...journal article 2023
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Pei, R. (author), Tarek-Bahgat, N. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Poly(3-hydroxybutyrate) (PHB) was accumulated in full-scale municipal waste activated sludge at pilot scale. After accumulation, the fate of the PHB-rich biomass was evaluated over two weeks as a function of initial pH (5.5, 7.0 and 10), and incubation temperature (25, 37 and 55 °C), with or without aeration. PHB became consumed under aerobic...journal article 2023
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Strubbe, Laurence (author), van Dijk, E.J.H. (author), Deenekamp, Pascalle J.M. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)In the pursuit of reducing carbon footprint and in view of increasing energy prices, energy efficiency is more important than ever before. Batch-wise operated aerobic granular sludge reactors consume up to 50% less energy compared to conventional activated sludge systems because pumping energy is reduced and mixing equipment is not needed....journal article 2023
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Tsui, To Hung (author), van Loosdrecht, Mark C.M. (author), Dai, Yanjun (author), Tong, Yen Wah (author)Biorefinery systems are playing pivotal roles in the technological support of resource efficiency for circular bioeconomy. Meanwhile, artificial intelligence presents great potential in handling scientific tasks of high-dimensional complexity. This review article scrutinizes the status of machine learning (ML) applications in four critical...review 2023
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Bahgat, T.M.S.M. (author), Wilfert, P.K. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author)The study evaluated the combined phosphorus, nitrogen, methane, and extracellular polymeric substances (EPS) recovery from aerobic granular sludge (AGS) wastewater treatment plants. About 30% of sludge organics are recovered as EPS and 25–30% as methane (≈260 ml methane/g VS) by integrating alkaline anaerobic digestion (AD). It was shown that...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Corbera Rubio, F. (author), Laureni, M. (author), Koudijs, N. (author), Müller, S. (author), van Alen, Theo (author), Schoonenberg, Frank (author), Lücker, Sebastian (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), van Halem, D. (author)Rapid sand filters (RSF) are an established and widely applied technology for groundwater treatment. Yet, the underlying interwoven biological and physical-chemical reactions controlling the sequential removal of iron, ammonia and manganese remain poorly understood. To resolve the contribution and interactions between the individual reactions...journal article 2023
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Soler Jofra, A. (author), Schmidtchen, Lisbeth (author), Olmo, Lluc (author), van Loosdrecht, Mark C.M. (author), Pérez, Julio (author)Hydroxylamine is a nitrogen intermediate of ammonium oxidizing bacteria (AOB) that can transiently accumulate during nitrification. The impact of hydroxylamine on aerobic ammonium oxidations is still obscure. In the present study the short and long term impact of hydroxylamine on partial nitritation granular sludge was investigated. Dissolved...journal article 2022
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Kulshreshtha, Y. (author), Vardon, P.J. (author), Meesters, G.M.H. (author), van Loosdrecht, Mark C.M. (author), Mota, Nelson (author), Jonkers, H.M. (author)The water-resistance of cow-dung has made it a widely used stabiliser in traditional earthen structures in several Asian and African countries. Multiple studies have shown an improvement in water-resistance with the addition of cow-dung, but none provides insight into this behaviour. The present study investigates the water-resistance behaviour...journal article 2022
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van den Berg, L. (author), Toja Ortega, S. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Aerobic granular sludge (AGS) is an advanced biofilm-based technology for wastewater treatment. Diffusion of substrates into the granules is a key aspect of this technology. Domestic wastewater contains soluble organic substrates of different sizes that could potentially diffuse into the granules. In this study, the relation between the...journal article 2022
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van den Berg, L. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), de Kreuk, M.K. (author)Granular sludge processes are frequently used in domestic and industrial wastewater treatment. The granule buoyant density and biomass density are important parameters for the design and operation of granular sludge reactors. Different methods to measure the granule density include the pycnometer method, the Percoll density gradient method,...journal article 2022
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Kouba, V. (author), D, Vejmelkova (author), E, Zwolsman (author), K, Hurkova (author), K, Navratilova (author), M, Laureni (author), Pabst, M. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Anammox bacteria enable efficient removal of nitrogen from sewage in processes involving partial nitritation and anammox (PN/A) or nitrification, partial denitrification, and anammox (N-PdN/A). In mild climates, anammox bacteria must be adapted to ≤15 °C, typically by gradual temperature decrease; however, this takes months or years. To...journal article 2022
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Kouba, V. (author), Bachmannová, Ch (author), Podzimek, T. (author), Lipovová, P. (author), van Loosdrecht, Mark C.M. (author)The adaptation of bacteria involved in the anaerobic ammonium oxidation (anammox) to low temperatures in the mainstream of WWTP will unlock substantial treatment savings. However, their adaptation mechanisms have begun to be revealed only very recently. This study reviewed the state-of-the-art knowledge on these mechanisms from -omics studies...review 2022
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Estevez Alonso, A. (author), Altamira-Algarra, Beatriz (author), Arnau-Segarra, César (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A...journal article 2022
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Cerruti, M. (author), Kim, J.H. (author), Pabst, Martin (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Purple bacteria (PPB), anoxygenic photoorganoheterotrophic organisms with a hyper-versatile metabolism and high biomass yields over substrate, are promising candidates for the recovery of nutrient resources from wastewater. Infrared light is a pivotal parameter to control and design PPB-based resource recovery. However, the effects of light...journal article 2022
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Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...journal article 2022