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Páez Watson, T.W. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)The understanding of microbial communities and the biological regulation of its members is crucial for implementation of novel technologies using microbial ecology. One poorly understood metabolic principle of microbial communities is resource allocation and biosynthesis. Resource allocation theory in polyphosphate accumulating organisms ...journal article 2023
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Ochs, Pascal (author), Martin, Ben (author), Germain-Cripps, Eve (author), Stephenson, Tom (author), van Loosdrecht, Mark C.M. (author), Soares, Ana (author)Over the past twenty years, various commercial technologies have been deployed to remove ammonia (NH<sub>4</sub>–N) from anaerobic digestion (AD) liquors. In recent years many anaerobic digesters have been upgraded to include a pre-treatment, such as the thermal hydrolysis process (THP), to produce more biogas, increasing NH<sub>4</sub>–N...journal article 2023
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Ziliani, Agustina (author), Bovio-Winkler, Patricia (author), Cabezas, Angela (author), Etchebehere, Claudia (author), Garcia, Hector A. (author), López-Vázquez, Carlos M. (author), Brdjanovic, Damir (author), van Loosdrecht, Mark C.M. (author), Rubio-Rincón, Francisco J. (author)Ca. Accumulibacter was the predominant microorganism (relative FISH bio-abundance of 67 ± 5%) in a lab-scale sequential batch reactor that accomplished enhanced biological phosphorus removal (EBPR) while using glucose and acetate as the carbon sources (1:1 COD-based ratio). Both organic compounds were completely anaerobically consumed. The...journal article 2023
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Tomas Martinez, S. (author), Zwolsman, Erwin J. (author), Merlier, Franck (author), Pabst, Martin (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author), Weissbrodt, D.G. (author)Polyphosphate accumulating organisms (PAOs) are responsible for enhanced biological phosphate removal (EBPR) from wastewater, where they grow embedded in a matrix of extracellular polymeric substances (EPS). EPSs comprise a mixture of biopolymers like polysaccharides or (glyco)proteins. Despite previous studies, little is known about the...journal article 2023
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Pei, R. (author), Tarek-Bahgat, N. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Poly(3-hydroxybutyrate) (PHB) was accumulated in full-scale municipal waste activated sludge at pilot scale. After accumulation, the fate of the PHB-rich biomass was evaluated over two weeks as a function of initial pH (5.5, 7.0 and 10), and incubation temperature (25, 37 and 55 °C), with or without aeration. PHB became consumed under aerobic...journal article 2023
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Tsui, To Hung (author), van Loosdrecht, Mark C.M. (author), Dai, Yanjun (author), Tong, Yen Wah (author)Biorefinery systems are playing pivotal roles in the technological support of resource efficiency for circular bioeconomy. Meanwhile, artificial intelligence presents great potential in handling scientific tasks of high-dimensional complexity. This review article scrutinizes the status of machine learning (ML) applications in four critical...review 2023
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Strubbe, Laurence (author), van Dijk, E.J.H. (author), Deenekamp, Pascalle J.M. (author), van Loosdrecht, Mark C.M. (author), Volcke, Eveline I.P. (author)In the pursuit of reducing carbon footprint and in view of increasing energy prices, energy efficiency is more important than ever before. Batch-wise operated aerobic granular sludge reactors consume up to 50% less energy compared to conventional activated sludge systems because pumping energy is reduced and mixing equipment is not needed....journal article 2023
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Kosar, Sadiye (author), Isik, Onur (author), Cicekalan, Busra (author), Gulhan, Hazal (author), Cingoz, Seyma (author), Yoruk, Mustafa (author), Ozgun, Hale (author), van Loosdrecht, Mark C.M. (author), Ersahin, Mustafa Evren (author)Achieving a neutral/positive energy balance without compromising discharge standards is one of the main goals of wastewater treatment plants (WWTPs) in terms of sustainability. Aerobic granular sludge (AGS) technology promises high treatment performance with low energy and footprint requirement. In this study, high-rate activated sludge (HRAS...journal article 2023
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Wang, Xiangyang (author), Shi, Chen (author), Hao, Xiaodi (author), van Loosdrecht, Mark C.M. (author), Wu, Yuanyuan (author)Wet-chemical approach is widely applied for phosphate recovery from incinerated ash of waste activated sludge (WAS), along with metals removed/recovered. The high contents of both aluminum (Al) and iron (Fe) in WAS-incinerated ash should be suitable for producing coagulants with some waste anions like Cl<sup>−</sup> and SO<sub>4</sub><sup>2−<...journal article 2023
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Kleikamp, H.B.C. (author), Grouzdev, Denis (author), - Schaasberg, P. (author), van Valderen, R.D. (author), van der Zwaan, R. (author), van de Wijgaart, R. (author), Lin, Y. (author), Abbas, B.A. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Pabst, Martin (author)The tremendous progress in sequencing technologies has made DNA sequencing routine for microbiome studies. Additionally, advances in mass spectrometric techniques have extended conventional proteomics into the field of microbial ecology. However, systematic studies that provide a better understanding of the complementary nature of these ...journal article 2023
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Hu, Zhi Yi (author), Lin, Yi Peng (author), Wang, Qing Ting (author), Zhang, Yi Xin (author), Tang, Jie (author), Wang, Shuai (author), van Loosdrecht, Mark C.M. (author), Zeng, Raymond Jianxiong (author), Zhang, Fang (author)By maintaining the cell integrity of waste activated sludge (WAS), structural extracellular polymeric substances (St-EPS) resist WAS anaerobic fermentation. This study investigates the occurrence of polygalacturonate in WAS St-EPS by combining chemical and metagenomic analyses that identify ∼22% of the bacteria, including Ferruginibacter and...journal article 2023
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Chen, L.M. (author), de Bruin, S. (author), Pronk, M. (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)Anaerobic and aerobic granular sludge processes are widely applied in wastewater treatment. In these systems, microorganisms grow in dense aggregates due to the production of extracellular polymeric substances (EPS). This study investigates the sialylation and sulfation of anionic glyconconjugates in anaerobic and aerobic granular sludges...journal article 2023
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Kim, Nam Kyeun (author), Bhattacharyya, Debes (author), van Loosdrecht, Mark C.M. (author), Lin, Y. (author)In the present research, a bio-based flame retardant (FR) was prepared using a biopolymer derived from wastewater sludge to improve the fire performance of polypropylene (PP). Extracellular polymeric substances (EPS), which were extracted from wastewater aerobic granular sludge, were absorbed into cellulose-based fibres, such as flax and...journal article 2023
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Ali, Muhammad (author), Singh, Yogesh (author), Fortunato, Luca (author), Rehman, Zahid Ur (author), Manjunath, Sarvajith (author), Vrouwenvelder, J.S. (author), Pronk, M. (author), van Loosdrecht, Mark C.M. (author), Saikaly, Pascal E. (author)This study describes a novel integration of aerobic granular sludge (AGS) with a gravity-driven membrane (GDM) system at a pilot scale with a treatment capacity of approximately 150 L per day to treat raw domestic wastewater. The treatment performance and energy consumption of the AGS-GDM system were compared to the neighboring full-scale...journal article 2023
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Yin, Xiaole (author), Chen, Xi (author), Jiang, Xiao Tao (author), Yang, Ying (author), Li, Bing (author), Shum, Marcus Ho Hin (author), Schmitt, H. (author), Cha, Chang Jun (author), van Loosdrecht, Mark C.M. (author)Surveillance of antibiotic resistance genes (ARGs) has been increasingly conducted in environmental sectors to complement the surveys in human and animal sectors under the "One-Health"framework. However, there are substantial challenges in comparing and synthesizing the results of multiple studies that employ different test methods and...journal article 2023
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Li, Zejiao (author), Wang, Jixiang (author), Liu, Jialin (author), Chen, Xingyu (author), Lei, Zhongfang (author), Yuan, Tian (author), Lee, Duu Jong (author), Lin, Y. (author), van Loosdrecht, Mark C.M. (author)Reducing CO<sub>2</sub> emission and energy consumption is crucial for the sustainable management of wastewater treatment plants (WWTPs). In this study, an algal-bacterial aerobic granular sludge (AGS) system was developed for efficient carbon (C) assimilation and nitrogen (N)/phosphorus (P) removal without the need for mechanical aeration....journal article 2023
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Olavarria, Karel (author), Sousa, Diana Z. (author), van Loosdrecht, Mark C.M. (author), Wahl, S.A. (author)Utilizing anaerobic metabolisms for the production of biotechnologically relevant products presents potential advantages, such as increased yields and reduced energy dissipation. However, lower energy dissipation may indicate that certain reactions are operating closer to their thermodynamic equilibrium. While stoichiometric analyses and...journal article 2023
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Bahgat, T.M.S.M. (author), Wilfert, P.K. (author), Korving, Leon (author), van Loosdrecht, Mark C.M. (author)The study evaluated the combined phosphorus, nitrogen, methane, and extracellular polymeric substances (EPS) recovery from aerobic granular sludge (AGS) wastewater treatment plants. About 30% of sludge organics are recovered as EPS and 25–30% as methane (≈260 ml methane/g VS) by integrating alkaline anaerobic digestion (AD). It was shown that...journal article 2023
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Li, Ji (author), Hao, Xiaodi (author), Shen, Zhan (author), Wu, Yuanyuan (author), van Loosdrecht, Mark C.M. (author)A high moisture content of waste activated sludge (WAS) associated with a low calorific value needs to be deeply dried towards self-supporting incineration. On the other hand, thermal energy with low temperature exchanged from treated effluent has great potential for drying sludge. Unfortunately, low-temperature drying of sludge seems to be...journal article 2023
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Zhang, Zi (author), Sato, Yugo (author), Dai, Ji (author), Chui, Ho kwong (author), Daigger, Glen (author), van Loosdrecht, Mark C.M. (author), Chen, Guanghao (author)Exploring alternative water sources and improving the efficiency of energy uses are crucial approaches to strengthening the water-energy securities and achieving carbon mitigations in sub(tropical) coastal cities. Seawater use for toilet flushing and district cooling systems is reportedly practical for achieving multiaspect benefits in Hong...journal article 2023