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Werker, A. (author), Pei, R. (author), Kim, Kevin (author), Moretto, Giulia (author), Estevez Alonso, A. (author), Vermeer, C.M. (author), Arcos-Hernandez, Monica (author), Dijkstra, Jelmer (author), de Vries, Erik (author)Past studies have repeatedly demonstrated the technical feasibility to produce polyhydroxyalkanoate (PHA) using bacterial biomass of mixed microbial cultures (MMCs). Commercial quality grades of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), PHBV, can be produced with control of average monomer composition. However, demonstration of PHBV...journal article 2023
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Pei, R. (author), Vicente-Venegas, Gerard (author), Tomaszewska-Porada, Agnieszka (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoates (PHAs) can be produced with municipal waste activated sludge from biological wastewater treatment processes. Methods of selective fluorescent staining with confocal laser scanning microscopy (CLSM) were developed and optimized to evaluate the distribution of PHA storage activity in this mixed culture activated sludge...journal article 2023
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Pei, R. (author), Tarek-Bahgat, N. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Poly(3-hydroxybutyrate) (PHB) was accumulated in full-scale municipal waste activated sludge at pilot scale. After accumulation, the fate of the PHB-rich biomass was evaluated over two weeks as a function of initial pH (5.5, 7.0 and 10), and incubation temperature (25, 37 and 55 °C), with or without aeration. PHB became consumed under aerobic...journal article 2023
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Estevez Alonso, A. (author), Arias-Buendía, María (author), Pei, R. (author), van Veelen, H. Pieter J. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Activated sludge from municipal wastewater treatment processes can be used directly for the production of biodegradable polyesters from the family of polyhydroxyalkanoates (PHAs). However, municipal activated sludge typically cannot accumulate PHAs to very high levels and often low yields of polymer produced on substrate are observed. In the...journal article 2022
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Estevez Alonso, A. (author), Altamira-Algarra, Beatriz (author), Arnau-Segarra, César (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)The developments of mixed culture polyhydroxyalkanoate production has been directed to maximize the biomass PHA content with limited attention to polymer quality. Direct comparison of PHA accumulation literature is challenging, and even regularly contradicting in reported results, due to underlying differences that are not well expressed. A...journal article 2022
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Pei, R. (author), Vicente-Venegas, Gerard (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Polyhydroxyalkanoate accumulation experiments at pilot scale were performed with fullscale municipal waste activated sludge. Development of biomass PHA content was quantified by thermogravimetric analysis. Over 48 h the biomass reached up to 0.49 ± 0.03 gPHA/gVSS (n=4). Samples were processed in parallel to characterise the distribution of...journal article 2022
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Bergman, R. (author), Abbas, A.E. (author), Jung, Sven (author), Werker, C. (author), de Reuver, Mark (author)Policymakers and analysts are heavily promoting data marketplaces to foster data trading between companies. Existing business model literature covers individually owned, multilateral data marketplaces. However, these particular types of data marketplaces hardly reach commercial exploitation. This paper develops business model archetypes for the...journal article 2022
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Pei, R. (author), Estevez Alonso, A. (author), Ortiz-Seco, Laura (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Municipal activated sludge can be used for polyhydroxyalkanoate (PHA) production, when supplied with volatile fatty acids. In this work, standardized PHA accumulation assays were performed with different activated sludge to determine (1) the maximum biomass PHA content, (2) the degree of enrichment (or volume-to-volume ratio of PHA...journal article 2022
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Estevez Alonso, A. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Microbial community-based polyhydroxyalkanoate (PHA) production has been demonstrated repeatedly at pilot scale. Ammonium, normally present in waste streams, might be oxidized by nitrifying bacteria resulting in additional aeration energy demand. The use of low dissolved oxygen (DO) concentrations allowed to reduce nitrifying rates by up to...journal article 2021
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Estevez Alonso, A. (author), Pei, R. (author), van Loosdrecht, Mark C.M. (author), Kleerebezem, R. (author), Werker, A. (author)Conversion of organic waste and wastewater to polyhydroxyalkanoates (PHAs) offers a potential to recover valuable resources from organic waste. Microbial community-based PHA production systems have been successfully applied in the last decade at lab- and pilot-scales, with a total of 19 pilot installations reported in the scientific...review 2021
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Aschenbrenner, D. (author), van Tol, D.H. (author), Rusak, Z. (author), Werker, C. (author)This paper proposes to use Virtual Reality scenarios to explore the reaction of stakeholders within an innovation process in the context of the introduction of robots working in close collaboration with users. The goal is to design the system upfront in such a way, that it is not perceived as a threat to the worker or his/her job. Within the...conference paper 2020
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Werker, C. (author)Recent disruptions caused by the digital age and increasing calls for Responsible Research and Innovation have overturned supposed certainties of how to assess research and innovation. This paper shows that: (1) Responsible Research and Innovation requires a system approach; and (2) digital transformation brings additional complications and...book chapter 2020
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Werker, C. (author), Hopp, Christian (author)Scholars’ balancing act between research and application leads to trade-offs between commercial and research output. Yet what some scholars may consider as poles apart might lead to super-additive outcomes for others. Based on a survey carried out at three leading European universities of technology we investigate the influence of scholars’...journal article 2020
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Hillerbrand, Rafaela (author), Werker, C. (author)In the applied sciences and in engineering there is often a significant overlap between work at universities and in industry. For the individual scholar, this may lead to serious conflicts when working on joint university–industry projects. Differences in goals, such as the university’s aim to disseminate knowledge while industry aims to...journal article 2019
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Werker, C. (author), Korzinov, Vladimir (author), Cunningham, S. (author)The development and deployment of technologies depend upon collaborations concurrently relying on proximity between partners. By employing publication data of German nanotechnology, we augment former findings on the relationship between proximity and collaboration in three ways. First, we shed light on how the various forms of proximity...journal article 2019
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Werker, C. (author), Ooms, Ward (author)Prior analyses of face-to-face contacts in collaborations have focused on one substitute only. Instead, we analyse various potential substitutes for face-to-face contacts in collaborations. Based on 45 interviews with academics from five leading European universities of technology our findings show that face-to-face contacts are closely...journal article 2019
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Ooms, Ward (author), Werker, C. (author), Caniëls, Marjolein (author)The proximity framework serves to analyse and understand how collaborations form and develop over time, and how these affect innovation and learning. The framework has inspired and informed empirical studies in several contexts, contributing to our understanding of the dynamics of dyadic collaborations, industrial clusters and districts, and...journal article 2018
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Ooms, Ward (author), Werker, C. (author), Hopp, Christian (author)We look into the question whether heterogeneity stemming from research orientation, gender, or disciplinary and cultural differences with their PhD supervisors helps or hampers academics’ careers. Based on a sample of 248 academics at two leading European universities of technology, we combine multinomial logit models and sequential logit...journal article 2018
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Werker, C. (author), Ubacht, J. (author), Ligtvoet, A. (author)Entrepreneurs are often envisioned as small private start-up firms operating against all odds. Here, we investigate how in the context of the Triple Helix various entrepreneurs form communities and drive institutional and technological change. To theoretically shape a socialized view of entrepreneurship, we use the Triple Helix approach. Our...journal article 2017
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Werker, C. (author), Ooms, Ward (author), Caniëls, Marjolein C J (author)Previous studies investigating proximity and collaboration have not clarified personal elements, such as working or communication style. Here, we show that personal proximity—close similarity in terms of personal traits and behavioral patterns—substantially affects the whole life cycle of research collaborations. We conduct a multi-case study...journal article 2016
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