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Jorda, J. (author), Suarez Mendez, C.A (author), Carnicer, M. (author), Ten Pierick, A. (author), Heijnen, J.J. (author), Van Gulik, W.M. (author), Ferrer, P. (author), Albiol, J. (author), Wahl, S.A. (author)
Background Several studies have shown that the utilization of mixed carbon feeds instead of methanol as sole carbon source is beneficial for protein production with the methylotrophic yeast Pichia pastoris. In particular, growth under mixed feed conditions appears to alleviate the metabolic burden related to stress responses triggered by protein...
journal article 2013
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Cruz, L.A.B. (author), Hebly, M. (author), Duong, G.H. (author), Wahl, S.A. (author), Pronk, J.T. (author), Heijnen, J.J. (author), Daran-Lapujade, P. (author), Van Gulik, W.M. (author)
Background Temperature strongly affects microbial growth, and many microorganisms have to deal with temperature fluctuations in their natural environment. To understand regulation strategies that underlie microbial temperature responses and adaptation, we studied glycolytic pathway kinetics in Saccharomyces cerevisiae during temperature changes....
journal article 2012