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document
Mendes, F. (author), Sieuwerts, S. (author), De Hulster, E. (author), Almering, M.J. (author), Luttik, M.A. (author), Pronk, J.T. (author), Smid, E.J. (author), Bron, P.A. (author), Daran-Lapujade, P. (author)
Mixed populations of Saccharomyces cerevisiae yeasts and lactic acid bacteria occur in many dairy, food, and beverage fermentations, but knowledge about their interactions is incomplete. In the present study, interactions between Saccharomyces cerevisiae and Lactobacillus delbrueckii subsp. bulgaricus, two microorganisms that co-occur in kefir...
journal article 2013
document
Boender, L.G.M. (author), Van Maris, A.J.A. (author), De Hulster, E.A.F. (author), Almering, M.J.H. (author), Van der Klei, I.J. (author), Veenhuis, M. (author), De Winde, J.H. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)
Extremely low specific growth rates (below 0.01 h?1) represent a largely unexplored area of microbial physiology. In this study, anaerobic, glucose-limited retentostats were used to analyse physiological and genome-wide transcriptional responses of Saccharomyces cerevisiae to cultivation at near-zero specific growth rates. While quiescence is...
journal article 2011
document
Livas, D. (author), Almering, M.J.H. (author), Daran, J.M. (author), Pronk, J.J. (author), Gancedo, J.M. (author)
Background The pattern of gene transcripts in the yeast Saccharomyces cerevisiae is strongly affected by the presence of glucose. An increased activity of protein kinase A (PKA), triggered by a rise in the intracellular concentration of cAMP, can account for many of the effects of glucose on transcription. In S. cerevisiae three genes, TPK1,...
journal article 2011
document
Boender, L.G.M. (author), Almering, M.J.H. (author), Dijk, M. (author), Van Maris, A.J.A. (author), De Winde, J.H. (author), Pronk, J.T. (author), Daran-Lapujade, P. (author)
Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and...
journal article 2011
document
Medina, V.G. (author), Almering, M.J.H. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author)
In anaerobic cultures of wild-type Saccharomyces cerevisiae, glycerol production is essential to reoxidize NADH produced in biosynthetic processes. Consequently, glycerol is a major by-product during anaerobic production of ethanol by S. cerevisiae, the single largest fermentation process in industrial biotechnology. The present study...
journal article 2009
document
Vuralhan, Z. (author), Luttik, M.A. (author), Tai, S.L. (author), Boer, V.M. (author), Morais, M.A. (author), Schipper, D. (author), Almering, M.J. (author), Kotter, P. (author), Dickinson, J.R. (author), Daran, J.M. (author), Pronk, J.T. (author)
journal article 2005
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