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Szymańska, K. (author), Odrozek, K. (author), Zniszczoł, A. (author), Torrelo, G. (author), Resch, V. (author), Hanefeld, U. (author), Jarzębskia, A.B. (author)
Acyltransferase from Mycobacterium smegmatis (MsAcT) immobilised in continuous-flow microchannel (30-50 ?m dia.) reactors with hierarchical pore structure (4 cm3/g total pore volume) enabled quantitative, full and rapid transesterification of neopentylglycol with ethyl acetate in a biphasic 50/50 % system in less than one minute. MsAcT was...
journal article 2016
document
Chen, B.S. (author), Resch, V. (author), Otten, L.G. (author), Hanefeld, U. (author)
The enantioselective Michael addition using water as both nucleophile and solvent has to date proved beyond the ability of synthetic chemists. Herein, the direct, enantioselective Michael addition of water in water to prepare important ?-hydroxy carbonyl compounds using whole cells of Rhodococcus strains is described. Good yields and excellent...
journal article 2014
document
Resch, V. (author), Hanefeld, U. (author)
Water is omnipresent and essential. Yet at the same time it is a rather unreactive molecule. The direct addition of water to C[double bond, length as m-dash]C double bonds is therefore a challenge not answered convincingly. In this perspective we critically evaluate the selectivity and the applicability of the different catalytic approaches for...
journal article 2014
document
Resch, V.A. (author), Jin, J. (author), Chen, B.S. (author), Hanefeld, U. (author)
The Michael hydratase – alcohol dehydrogenase (MhyADH) from Alicycliphilus denitrificans was previously identified as a bi-functional enzyme performing a hydration of ?,?-unsaturated ketones and subsequent oxidation of the formed alcohols. The investigations of the bi-functionality were based on a spectrophotometric assay and an activity...
journal article 2014
document
Resch, V. (author), Seidler, C. (author), Chen, B.S. (author), Degeling, I. (author), Hanefeld, U. (author)
The use of water as a nucleophile for Michael additions is still a challenge in organic chemistry. In this report we describe the use of amino acids as catalysts for the Michael addition of water to ?,?-unsaturated ketones. All 20 proteinogenic amino acids were screened and L-lysine was identified as the best candidate. To obtain a better...
journal article 2013
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Chen, B.S. (author), Otten, L.G. (author), Resch, V. (author), Muyzer, G. (author), Hanefeld, U. (author)
Rhodococcus rhodochrous ATCC 17895 possesses an array of mono- and dioxygenases, as well as hydratases, which makes it an interesting organism for biocatalysis. R. rhodochrous is a Gram-positive aerobic bacterium with a rod-like morphology. Here we describe the features of this organism, together with the complete genome sequence and annotation....
journal article 2013
document
Schrittwieser, J.H. (author), Resch, V. (author)
Alkaloids are not only one of the most intensively studied classes of natural products, their wide spectrum of pharmacological activities also makes them indispensable drug ingredients in both traditional and modern medicine. Among the methods for their production, biotechnological approaches are gaining importance, and biocatalysis has emerged...
journal article 2013
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