Searched for: collection%3Air
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Livas, D. (author), Almering, M.J.H. (author), Daran, J.M. (author), Pronk, J.J. (author), Gancedo, J.M. (author)
Background The pattern of gene transcripts in the yeast Saccharomyces cerevisiae is strongly affected by the presence of glucose. An increased activity of protein kinase A (PKA), triggered by a rise in the intracellular concentration of cAMP, can account for many of the effects of glucose on transcription. In S. cerevisiae three genes, TPK1,...
journal article 2011
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Boender, L.G.M. (author), Almering, M.J.H. (author), Dijk, M. (author), Van Maris, A.J.A. (author), De Winde, J.H. (author), Pronk, J.T. (author), Daran-Lapujade, P. (author)
Cultivation methods used to investigate microbial calorie restriction often result in carbon and energy starvation. This study aims to dissect cellular responses to calorie restriction and starvation in Saccharomyces cerevisiae by using retentostat cultivation. In retentostats, cells are continuously supplied with a small, constant carbon and...
journal article 2011
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Chen, J.C. (author), Pronk, T.M.C. (author), Van de Wouw, A. (author)
Document(en) uit de collectie Chemische Procestechnologie
report 2011
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Daran, J.M. (author), Pronk, J.T. (author), Driessen, A.J.M. (author), Nijland, J.G. (author), Lamboo, F. (author), Puig-Martinez, M. (author), Veiga, T. (author), Gombert, A.K. (author)
Penicillium chrysogenum is widely used as an industrial antibiotic producer, in particular in the synthesis of ß-lactam antibiotics such as penicillins and cephalosporins. In industrial processes, oxalic acid formation leads to reduced product yields. Moreover, precipitation of calcium oxalate complicates product recovery. We observed oxalate...
journal article 2011
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Pronk, J.T. (author), Van Maris, A.J.A. (author), Guadalupe Medina, V.G. (author)
The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH- dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising...
patent 2011
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Pronk, C. (author)
This paper presents a study on modeling and verifying the kernel of Real-Time Operating Systems (RTOS). The study will show advances in formally verifying such an RTOS both by refinement and by model checking approaches. This work fits in the context of Hoare’s verification challenge. Several real-time operating systems will be discussed...
report 2010
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Zelle, R.M. (author), Harrison, J.C. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Malic enzyme catalyzes the reversible oxidative decarboxylation of malate to pyruvate and CO2. The Saccharomyces cerevisiae MAE1 gene encodes a mitochondrial malic enzyme whose proposed physiological roles are related to the oxidative, malate-decarboxylating reaction. Hitherto, the inability of pyruvate carboxylase-negative (Pyc–) S. cerevisiae...
journal article 2010
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Woldekidan, M.F. (author), Huurman, M. (author), Pronk, A.C. (author)
Linear viscoelastic properties of bituminous binders for short loading times are analyzed using dynamic mechanical analysis methods. Dynamic Shear Rheometer (DSR) test with parallel plate (PP) configuration is widely used for this purpose. Due to the complex stress distribution over the cross-section of the test sample, application of this setup...
conference paper 2010
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Zelle, R.M. (author), Trueheart, J. (author), Harrison, J.C. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Pyruvate carboxylase is the sole anaplerotic enzyme in glucose-grown cultures of wild-type Saccharomyces cerevisiae. Pyruvate carboxylase-negative (Pyc–) S. cerevisiae strains cannot grow on glucose unless media are supplemented with C4 compounds, such as aspartic acid. In several succinate-producing prokaryotes, phosphoenolpyruvate...
journal article 2010
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Zelle, R.M. (author), De Hulster, E. (author), Kloezen, W. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter(-1) of malate at a yield of 0.42 mol (mol glucose)(-1) in calcium carbonate-buffered shake flask cultures. With shake flasks, process parameters that are important for scaling up this...
journal article 2010
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Taverne, P. (author), Pronk, C. (author)
Accepted for publication in the Proceedings of the 11th International Conference on Formal Engineering Methods, ICFEM 2009. This paper presents a case study in modeling and verifying a POSIX-like file store for Flash memory. This work fits in the context of Hoare’s verification challenge and, in particular, Joshi and Holzmann’s mini-challenge to...
report 2009
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Zelle, R.M. (author), De Hulster, E. (author), Kloezen, W. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
A recent effort to improve malic acid production by Saccharomyces cerevisiae by means of metabolic engineering resulted in a strain that produced up to 59 g liter?1 of malate at a yield of 0.42 mol (mol glucose)?1 in calcium carbonate-buffered shake flask cultures. With shake flasks, process parameters that are important for scaling up this...
journal article 2009
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Medina, V.G. (author), Almering, M.J.H. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author)
In anaerobic cultures of wild-type Saccharomyces cerevisiae, glycerol production is essential to reoxidize NADH produced in biosynthetic processes. Consequently, glycerol is a major by-product during anaerobic production of ethanol by S. cerevisiae, the single largest fermentation process in industrial biotechnology. The present study...
journal article 2009
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Hazelwood, L.A. (author), Walsh, M.C. (author), Pronk, J.T. (author), Daran, J.M. (author)
The hop plant, Humulus lupulus L., has an exceptionally high content of secondary metabolites, the hop -acids, which possess a range of beneficial properties, including antiseptic action. Studies performed on the mode of action of hop iso--acids have hitherto been restricted to lactic acid bacteria. The present study investigated molecular...
journal article 2009
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Hazelwood, L.A. (author), Walsh, M.C. (author), Luttik, M.A.H. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), Daran, J.M. (author)
OA Fund TU Delft Accumulation of glycogen and trehalose in nutrient-limited cultures of Saccharomyces cerevisiae is negatively correlated with the specific growth rate. Additionally, glucose-excess conditions (i.e., growth limitation by nutrients other than glucose) are often implicated in high-level accumulation of these storage carbohydrates....
journal article 2009
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Boender, L.G.M. (author), De Hulster, E.A.F. (author), Van Maris, A.J.A. (author), Daran-Lapujade, P.A.S. (author), Pronk, J.T. (author)
Growth at near-zero specific growth rates is a largely unexplored area of yeast physiology. To investigate the physiology of Saccharomyces cerevisiae under these conditions, the effluent removal pipe of anaerobic, glucose-limited chemostat culture (dilution rate, 0.025 h–1) was fitted with a 0.22-µm-pore-size polypropylene filter unit. This...
journal article 2009
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Abbott, D.A. (author), Zelle, R.M. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
To meet the demands of future generations for chemicals and energy and to reduce the environmental footprint of the chemical industry, alternatives for petrochemistry are required. Microbial conversion of renewable feedstocks has a huge potential for cleaner, sustainable industrial production of fuels and chemicals. Microbial production of...
journal article 2009
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Abbott, D.A. (author), Suir, E. (author), Duong, G.H. (author), De Hulster, E. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Industrial production of lactic acid with the current pyruvate decarboxylase-negative Saccharomyces cerevisiae strains requires aeration to allow for respiratory generation of ATP to facilitate growth and, even under nongrowing conditions, cellular maintenance. In the current study, we observed an inhibition of aerobic growth in the presence of...
journal article 2009
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Harris, D.M. (author), Van der Krogt, Z.A. (author), Klaassen, P. (author), Raamsdonk, L.M. (author), Hage, S. (author), Van den Berg, M.A. (author), Bovenberg, R.A.L. (author), Pronk, J.T. (author), Daran, J.M. (author)
OA Fund TU Delft Background Since the discovery of the antibacterial activity of penicillin by Fleming 80 years ago, improvements of penicillin titer were essentially achieved by classical strain improvement through mutagenesis and screening. The recent sequencing of Penicillium chrysogenum strain Wisconsin1255-54 and the availability of...
journal article 2009
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Knijnenburg, T.A. (author), Daran, J.M.G. (author), Van den Broek, M.A. (author), Daran-Lapujade, P.A.S. (author), De Winde, J.H. (author), Pronk, J.T. (author), Reinders, M.J.T. (author), Wessels, L.F.A. (author)
OA Fund TU delft Background: Microorganisms adapt their transcriptome by integrating multiple chemical and physical signals from their environment. Shake-flask cultivation does not allow precise manipulation of individual culture parameters and therefore precludes a quantitative analysis of the (combinatorial) influence of these parameters on...
journal article 2009
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