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Malic Acid Production by Saccharomyces cerevisiae: Engineering of Pyruvate Carboxylation, Oxaloacetate Reduction, and Malate Export

Zelle, R.M. (author), De Hulster, E. (author), Van Winden, W.A. (author), De Waard, P. (author), Dijkema, C. (author), Winkler, A.A. (author), Geertman, J.M. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

Malic acid is a potential biomass-derivable "building block" for chemical synthesis. Since wild-type Saccharomyces cerevisiae strains produce only low levels of malate, metabolic engineering is required to achieve efficient malate production with this yeast. A promising pathway for malate production from glucose proceeds via carboxylation of...

journal article 2008

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The Ehrlich Pathway for Fusel Alcohol Production: A Century of Research on Saccharomyces cerevisiae Metabolism

Hazelwood, L.A. (author), Daran, J.M. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Dickinson, J.R. (author)

journal article 2008

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Transcription factor control of growth rate dependent genes in Saccharomyces cerevisiae: A three factor design

Fazio, A. (author), Jewett, M.C. (author), Daran-Lapujade, P. (author), Mustacchi, R. (author), Usaite, R. (author), Pronk, J.T. (author), Workman, C.T. (author), Nielsen, J. (author)

journal article 2008

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New insights into the Saccharomyces cerevisiae fermentation switch: Dynamic transcriptional response to anaerobicity and glucose-excess

Van Den Brink, J. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), De Winde, J.H. (author)

OA Fund

journal article 2008

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Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis

Tai, S.L. (author), Daran-Lapujade, P. (author), Walsh, M.C. (author), Pronk, J.T. (author), Daran, J.M. (author)

Effects of suboptimal temperatures on transcriptional regulation in yeast have been extensively studied in batch cultures. To eliminate indirect effects of specific growth rates that are inherent to batch-cultivation studies, genome-wide transcriptional responses to low temperatures were analyzed in steady-state chemostats, grown at a fixed...

journal article 2007

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The fluxes through glycolytic enzymes in Saccharomyces cerevisiae are predominantly regulated at posttranscriptional levels

Daran, J.M. (author), Bakker, B.M. (author), Pronk, J.T. (author), Westerhoff, H.V. (author), De Winde, J.H. (author), Heck, A.J.R. (author), Slijper, M. (author), De Groot, M.J.L. (author), Luttik, M.A.H. (author), Van Gulik, W.M. (author), Rossell, S. (author), Daran-Lapujade, P. (author)

Metabolic fluxes may be regulated ‘‘hierarchically,’’ e.g., by changes of gene expression that adjust enzyme capacities (Vmax) and/or ‘‘metabolically’’ by interactions of enzymes with substrates, products, or allosteric effectors. In the present study, a method is developed to dissect the hierarchical regulation into contributions by...

journal article 2007

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Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of L-Arabinose

Wisselink, H.W. (author), Toirkens, M.J. (author), Del Rosario Franco Berriel, M. (author), Winkler, A.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)

For cost-effective and efficient ethanol production from lignocellulosic fractions of plant biomass, the conversion of not only major constituents, such as glucose and xylose, but also less predominant sugars, such as L-arabinose, is required. Wild-type strains of Saccharomyces cerevisiae, the organism used in industrial ethanol production,...

journal article 2007

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Generic and specific transcriptional responses to different weak organic acids in anaerobic chemostat cultures of Saccharomyces cerevisiae.

Abbott, D.A. (author), Knijnenburg, T.A. (author), De Poorter, L.M. (author), Reinders, M.J. (author), Pronk, J.T. (author), Van Maris, A.J. (author)

Transcriptional responses to four weak organic acids (benzoate, sorbate, acetate and propionate) were investigated in anaerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae. To enable quantitative comparison of the responses to the acids, their concentrations were chosen such that they caused a 50% decrease of the biomass...

journal article 2007

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Development of Efficient Xylose Fermentation in Saccharomyces cerevisiae: Xylose Isomerase as a Key Component

Van Maris, A.J.A. (author), Winkler, A.A. (author), Kuyper, M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

Metabolic engineering of Saccharomyces cerevisiae for ethanol production from d-xylose, an abundant sugar in plant biomass hydrolysates, has been pursued vigorously for the past 15 years. Whereas wild-type S. cerevisiae cannot ferment d-xylose, the ketoisomer d-xylulose can be metabolised slowly. Conversion of d-xylose into d-xylulose is...

journal article 2007

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Control of the Glycolytic Flux in Saccharomyces cerevisiae Grown at Low Temperature: A multi-level analysis in anaerobic chemostat cultures

Tai, S.L. (author), Daran-Lapujade, P. (author), Luttik, M.A. (author), Walsh, M.C. (author), Diderich, J.A. (author), Krijger, G.C. (author), Van Gulik, W.M. (author), Pronk, J.T. (author), Daran, J.M. (author)

journal article 2007

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Exploiting combinatorial cultivation conditions to infer transcriptional regulation

Knijnenburg, T.A. (author), De Winde, J.H. (author), Daran, J.M. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), Reinders, M.J.T. (author), Wessels, L.F.A. (author)

journal article 2007

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Exploiting combinatorial cultivation conditions to infer transcriptional regulation

Knijnenburg, T.A. (author), De Winde, J.H. (author), Daran, J.M. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author), Reinders, M.J.T. (author), Wessels, L.F.A. (author)

journal article 2007

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Alcoholic fermentation of carbon sources in biomass hydrolysates by Saccharomyces cerevisiae: Current status

Van Maris, A.J.A. (author), Abbott, D.A. (author), Bellissimi, E. (author), Van den Brink, J. (author), Kuyper, M. (author), Lutik, M.A.H. (author), Wisselink, H.W. (author), Scheffers, W.A. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

journal article 2006

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Fluidized bed heat exchangers to prevent fouling in ice slurry systems and industrial crystallizers

Pronk, P. (author)

Ozon layer depletion and global warming by synthetic refrigerants forces refrigeration industries to switch over to natural but hazardous refrigerants like ammonia and hydrocarbons. A promising technology to safely use the latter refrigerants is the application of indirect refrigeration systems with ice slurry as heat transfer fluid. Ice slurry,...

doctoral thesis 2006

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When transcriptome meets metabolome: Fast cellular responses of yeast to sudden relief of glucose limitation

Heijnen, J.J. (author), Daran, J.M. (author), Pronk, J.T. (author), Daran-Lapujade, P. (author), Knijnenburg, T.A. (author), Ras, C. (author), Ten Pierick, A. (author), Akmering, M.J. (author), Van Winden, W.A. (author), Kresnowati, M.T. (author)

Within the first 5 min after a sudden relief from glucose limitation, Saccharomyces cerevisiae exhibited fast changes of intracellular metabolite levels and a major transcriptional reprogramming. Integration of transcriptome and metabolome data revealed tight relationships between the changes at these two levels. Transcriptome as well as...

journal article 2006

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Metabolic engineering of xylose fermenting eukaryotic cells

Winkler, A.A. (author), Kuyper, S.M. (author), De Laat, W.T. (author), Van Dijken, J.P. (author), Pronk, J.T. (author)

The present invention relates to further genetic modifications in eukaryotic host cells that have been transformed to express a xylose isomerase that confers the host cell the ability of isomerising xylose to xylulose. The further genetic modifications are aimed at improving the efficiency of xylose metabolism and include e.g. reduction of...

patent 2006

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Industrial Biotechnology: Drive my car

Pronk, J.T (author)

Dies Natalis 164

public lecture 2006

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Carbonic anhydrase (Nce103p): An essential biosynthetic enzyme for growth of Saccharomyces cerevisiae at atmospheric carbon dioxide pressure

Aguilera, J. (author), Van Dijken, J.P. (author), De Winde, J.H. (author), Pronk, J.T. (author)

journal article 2005

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Prolonged selection in aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae causes a partial loss of glycolytic capacity

Jansen, M.L.A. (author), Diderich, J.A. (author), Mashego, M. (author), Hassane, A. (author), de Winde, J.H. (author), Daran-Lapujade, P. (author), Pronk, J.T. (author)

journal article 2005

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Physiological Characterization of the ARO10-Dependent, Broad-Substrate-Specificity 2-Oxo Acid Decarboxylase Activity of Saccharomyces cerevisiae

Vuralhan, Z. (author), Luttik, M.A. (author), Tai, S.L. (author), Boer, V.M. (author), Morais, M.A. (author), Schipper, D. (author), Almering, M.J. (author), Kotter, P. (author), Dickinson, J.R. (author), Daran, J.M. (author), Pronk, J.T. (author)

journal article 2005