Assessing yeast proteome dynamics using high-resolution mass spectrometry

Mass spectrometry-based cellular proteomics has taken a prominent role in many fields of research, including life sciences, biotechnology and microbial ecology. Still, advanced mass spectrometry-based proteomics methods are not routinely applied to microbes. The cell factory and model organism Saccharomyces cerevisiae, for example, is very well...

Cellular balancing under dynamic conditions: A systems biology-based discovery using experimental and modelling approaches

Saccharomyces cerevisiae, also known as baker’s yeast, is a robust microorganism frequently used in industrial biotechnology. The scale of its applications ranges from several millilitres for research and process development in the lab to hundreds of cubic meters for cultivation in industrial production processes. In large-scale reactors mixing...

Engineering and characterization of alternative configurations of glycolysis in Saccharomyces cerevisiae

One of the most conserved and central parts of metabolism is the pathway of glycolysis, that breaks down hexose sugars. While multiple variants of glycolytic pathway exist, the Emden-Meyerhof-Parnas pathway which converts glucose to two molecules of pyruvate with the net gain of two ATP and two reduced NADH moieties is the one predominantly...

Single-step in vivo assembly of synthetic chromosomes with a modular design for engineering yeast metabolism

Microorganisms have been used by humanity since ancient times to produce fermented food and beverages such as wine, bread and beer. With the current molecular biology techniques, it is not only possible to increase microbial performance for the production of native products, but also to adapt microorganism to make heterologous or even novel-to...

Modular engineering of synthetic glycolytic pathways in Saccharomyces cerevisiae

Already for millennia, microbial fermentation is used for the production of dairy products, alcoholic beverages and bread. In the last decades, the field of biotechnology has tremendously expanded and nowadays, a wide range of compounds ranging from biofuels to chemicals and pharmaceuticals is produced using microbial cell factories. The...

Physiology of aerobic yeast cultures under industrially relevant conditions

In industrial biotechnology, micro-organisms are used for making a wide range of products, including neutraceuticals, transport fuels, pharmaceuticals and plastics. The raw materials for these microbial processes are often sugars derived from natural resources. Industrial biotechnology offers an advantage with respect to the petrochemical...

Adaptation of Saccharomyces yeasts to low and fluctuating temperatures