Development and application of ultrafast scanning electron microscopy

Scanning electron microscopes (SEMs) can capture detail on the single nanometer length scale through the interaction of a tightly focused electron beam with a sample, but this impressive spatial resolution is not matched with a capability to resolve dynamic processes on the ultrafast time scale. A variety of processes occur at nanosecond and...

Optical tools towards the improvement of optogenetic stimulation

Optogenetics is a powerful addition to the spectrum of techniques available in neuroscience to investigate neurophysiology and unravel how neural circuit structure is related to circuit function. This technique relies on introducing lightsensitive proteins or molecules as actuators to transduce an optical signal into a physiological perturbation...

Manipulation of fluorescence using low energy electron beams for correlative microscopy

Combining light and electron microscopy in an integrated system allows for the combination of two different sorts of information in an automated fashion. This type of imaging, called integrated correlative light and electron microscopy (CLEM), is used for imaging of biological specimen and allows us to put the biomolecular context provided by...

Integrated Array Tomography: Development and Applications of a Workflow for 3D Correlative Light and Electron Microscopy

Multi-modal imaging techniques have become essential for better understanding fundamental questions in cell biology such as disease progression. While individual microscopy methods have rapidly advanced in recent years, the information content of any one imaging technique is limited to the type of contrast that particular technique is sensitive...

Creation of electron pulses with a laser-triggered micro fabricated electron beam deflector

This thesis is dedicated to the development of a beam blanker for Ultrafast Electron Microscopy. Ultrafast electron microscopy aims to resolve structural dynamics at the nanometer and (sub) picosecond time scale. In these temporal and spatial scales many important processes in physics, chemistry and biology do occur. Examples of these are the...

Cathodoluminescence Microscopy of Nanostructures on Transparent Substrates

Cathodoluminescence (CL), the excitation of light by an electron beam, has gained attention as an analysis tool for investigating the optical response of a structure, at a resolution that approaches that in electron microscopy, in the nanometer range. However, the application possibilities are limited because the use of transparent substrates,...