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Knibbe, E. (author)One of the most conserved and central parts of metabolism is the pathway of glycolysis, that breaks down hexose sugars. While multiple variants of glycolytic pathway exist, the Emden-Meyerhof-Parnas pathway which converts glucose to two molecules of pyruvate with the net gain of two ATP and two reduced NADH moieties is the one predominantly...doctoral thesis 2022
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de Valk, S.C. (author)In living cells, transport proteins allow for the translocation of molecules across biological membranes that are otherwise impermeable to charged and polar solutes. These membrane associated proteins play an essential role in the uptake of substrate molecules and export of metabolic products, as well as in the maintenance of electrochemical...doctoral thesis 2022
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Wronska, A.K. (author)Every century brings its own challenges, but the 21st century is the first in which a global transition towards circularity is required to ensure human existence on this planet. Exhaustion of planetary resources, such as oil and rare elements, must be prevented and sustainable circular value chains introduced into our industry and economy. In...doctoral thesis 2022
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Jürgens, H. (author)Microorganisms, enhanced by genetic engineering, are important cell factories for conversion of renewable feedstocks into fuels, chemicals, biomaterials, nutraceuticals and drugs. Microbial synthesis of these products from chemically simple carbon substrates often requires a net input of free energy in the form of ATP, which is typically...doctoral thesis 2022
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Bouwknegt, J. (author)Products resulting from alcoholic fermentation by microorganisms have been used by mankind for over 11,000 years. Alcoholic fermentation is involved in production of bread, alcoholic beverages such as wine and beer, distilled spirits such as vodka and rum, and bio-ethanol. The popularity of the most intensively used microorganism for these...doctoral thesis 2022
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Else-Hassing, J. (author)Over the past century, the world population has increased fourfold. This increase in human population is accompanied by an increased demand in food, water, energy and consumer goods. The resulting intensification of agriculture, deforestation, emission of green-house gasses and high utilization of natural compounds such as coal, minerals, metals...doctoral thesis 2022
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Postma, E.D. (author)Microorganisms have been used by humanity since ancient times to produce fermented food and beverages such as wine, bread and beer. With the current molecular biology techniques, it is not only possible to increase microbial performance for the production of native products, but also to adapt microorganism to make heterologous or even novel-to...doctoral thesis 2022
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Wiersma, S.J. (author)The yeast Saccharomyces cerevisiae has been used by humans for many centuries in microbial fermentation processes for the production of, for example, bread and alcoholic beverages. This long history of use and, in addition, its fast growth, its ability to rapidly convert sugars into ethanol and the ease with which it can be genetically modified,...doctoral thesis 2021
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Dekker, W.J.C. (author)Saccharomyces cerevisiae is a natural producer of ethanol and industrial strains can produce ethanol at high volumetric rates and near-theoretical yields. In addition to its fast fermentative metabolism, its GRAS (generally recognized as safe) status, ease of genetic engineering, tolerance to low pH and high ethanol concentrations contribute to...doctoral thesis 2021
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Perli, T. (author)The increase in world population together with an unsustainable and fossil fuel-based economy are at the root of the significant increase in the average global temperature since the pre-industrial era. Switching to a more sustainable and circular bio-based economy is one of the main pillars included in the Paris agreement aimed at reducing green...doctoral thesis 2021
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Baldi, N. (author)In the past decades, biotechnology has become ever more prominent in our society. On supermarket shelves, biotechnological staple products such beer, bread and wine are accompanied by enzyme-containing detergents and soaps with biotechnologically produced fragrances, all of which are possibly packaged with bio-derived plastics. In the medical...doctoral thesis 2021
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Boonekamp, F.J. (author)Already for millennia, microbial fermentation is used for the production of dairy products, alcoholic beverages and bread. In the last decades, the field of biotechnology has tremendously expanded and nowadays, a wide range of compounds ranging from biofuels to chemicals and pharmaceuticals is produced using microbial cell factories. The...doctoral thesis 2020
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Liu, Y. (author)The progress of modern biotechnology has enabled the development of fermentation processes for the production of fuels and chemicals from renewable feedstocks. The current fermentation processes for bio-based production commonly start with a growth phase of the microorganisms followed by a production phase. This implies that biomass formation...doctoral thesis 2020
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Brouwers, N. (author)The first production of alcoholic beverages by fermentation of plant-derived materials occurred many thousands of years ago. In the 15th century, production of lager beer with pale barley malt, soft water and Saaz noble hops, first resulted in pilsner, a clear golden, crispy tasting beer. Today, this type of lager beer is the most produced...doctoral thesis 2020
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Valk, L.C. (author)Over the past decade the demand for technology which can make our global economy more sustainable has increased exponentially. In the field of biotechnology, one of the many advancements is focussed on the conversion of agricultural waste streams towards commodity chemicals. Plant biomass can be an environmental and sustainable alternative for...doctoral thesis 2020
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Hakkaart, X.D.V. (author)In industrial biotechnology, micro-organisms are used for making a wide range of products, including neutraceuticals, transport fuels, pharmaceuticals and plastics. The raw materials for these microbial processes are often sugars derived from natural resources. Industrial biotechnology offers an advantage with respect to the petrochemical...doctoral thesis 2019
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Gorter de Vries, A.R. (author)Lager beer, also referred to as Pilsner, is the most popular alcoholic beverage in the world, with an annual consumption of almost 200 billion litres per year. To make lager beer, brewer’s wort is fermented with the yeast <i style="mso-bidi-font-style:normal"...doctoral thesis 2019
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Brickwedde, A. (author)haploid S. cerevisiae laboratory strain of the CEN.PK family. The performance of the constructed hybrid, strain IMS0408, was then compared to those of its parents in anaerobic batch cultures grown on different media and at different temperatures. While S. eubayanus displayed significantly higher growth rates than S. cerevisiae in anaerobic batch...doctoral thesis 2019
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Bracher, J.M. (author)Nearly 200 parties signed and committed to the Paris agreement in 2017, which comprises the long-term goal to keep the average global temperature increase well below 2 degrees above pre-industrial levels. Virtually all possible scenarios drafted to reach this goal include a strongly increased use of biofuels for transport by land, sea and air....doctoral thesis 2019
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Marques, W.L. (author)Contemporary society heavily depends on fossil sources. The energy and materials derived from fossil reserves were major contributors to the acceleration and intensification of agriculture and industry over the past 100 years. Such reserves are finite, hence, after expanding geographically, our economy is now consuming natural reserves that...doctoral thesis 2018