Searched for: department%3A%22Biotechnology%22
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Gonzalez-Martinez, A. (author), Rodriguez-Sanchez, A. (author), Lotti, T. (author), Garcia-Ruiz, M.J. (author), Gonzalez-Lopez, J. (author), Van Loosdrecht, M.C.M. (author)
The bacterial community structure of 10 different wastewater treatment systems and their influents has been investigated through pyrosequencing, yielding a total of 283486 reads. These bioreactors had different technological configurations: conventional activated sludge (CAS) systems and very highly loaded A-stage systems. A-stage processes are...
journal article 2016
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Fernandez Fueyo, E. (author), Ruiz-Duenas, F.J. (author), Lopez-Lucendo, M.F. (author), Perez-Boada, M. (author), Rencorat, J. (author), Gutierrez, A. (author), Pisabarro, A.G. (author), Ramirez, L. (author), Martinez, A.T. (author)
Background: Pleurotus ostreatus is the second edible mushroom worldwide, and a model fungus for delignification applications, with the advantage of growing on woody and nonwoody feedstocks. Its sequenced genome is available, and this gave us the opportunity to perform proteomic studies to identify the enzymes overproduced in lignocellulose...
journal article 2016
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Cornelio da Silva, Constança (author)
The well-known volatility of petroleum price and, subsequently, of its derived products has long time urged the attention for the discovery of alternatives to fossil oil. More recently, the dichotomy food-fuel, has redirected the research towards second generation feedstocks, in which biomass residues are included. In line with this, a...
student report 2016
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Van Rossum, H.M. (author), Kozak, B.U. (author), Pronk, J.T. (author), Van Maris, A.J.A. (author)
Saccharomyces cerevisiae is an important industrial cell factory and an attractive experimental model for evaluating novel metabolic engineering strategies. Many current and potential products of this yeast require acetyl coenzyme A (acetyl-CoA) as a precursor and pathways towards these products are generally expressed in its cytosol. The native...
journal article 2016
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van den Broeck, W. (author)
Fossil fuels will run out in the foreseeable future. Out entire economic system is build upon fossil fuels and building up another base for this economic system is challenging. The only alternative for making materials that normally are derived from fossil fuels is the Bio-Based Economy. Materials in this way get made from biomass, such as sugar...
master thesis 2016
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Kuijpers, N.G.A. (author)
In the past few decades, concerns on climate change have contributed to the development of biotechnological alternatives for petrochemical processes. In order to make these processes economically competitive, there has been an intensive search for improved, more efficient production organisms. In the past decade, genetic engineering of...
doctoral thesis 2016
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Bisschops, M.M.M. (author)
doctoral thesis 2016
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Hamidi, A. (author)
Haemophilus influenzae type b (Hib) conjugate vaccine is a safe and effective vaccine that can prevent meningitis and pneumonia caused by Hib disease. Hib vaccine is recommended for all children under 5 years. Despite the availability of safe and effective Hib vaccines since early 1987, Gambia was the only low-income country that had introduced...
doctoral thesis 2016
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Szymańska, K. (author), Odrozek, K. (author), Zniszczoł, A. (author), Torrelo, G. (author), Resch, V. (author), Hanefeld, U. (author), Jarzębskia, A.B. (author)
Acyltransferase from Mycobacterium smegmatis (MsAcT) immobilised in continuous-flow microchannel (30-50 ?m dia.) reactors with hierarchical pore structure (4 cm3/g total pore volume) enabled quantitative, full and rapid transesterification of neopentylglycol with ethyl acetate in a biphasic 50/50 % system in less than one minute. MsAcT was...
journal article 2016
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Prest, E.I.E.D. (author), Hammes, F. (author), Van Loosdrecht, M.C.M. (author), Vrouwenvelder, J.S. (author)
Biological stability of drinking water refers to the concept of providing consumers with drinking water of same microbial quality at the tap as produced at the water treatment facility. However, uncontrolled growth of bacteria can occur during distribution in water mains and premise plumbing, and can lead to hygienic (e.g., development of...
journal article 2016
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Suarez Mendez, C.A. (author), Hanemaaijer, M. (author), Ten Pierick, A. (author), Wolters, J.C. (author), Heijnen, J.J. (author), Wahl, S.A. (author)
13C labeling experiments in aerobic glucose limited cultures of Saccharomyces cerevisiae at four different growth rates (0.054; 0.101, 0.207, 0.307 h?1) are used for calculating fluxes that include intracellular cycles (e.g., storage carbohydrate cycles, exchange fluxes with amino acids), which are rearranged depending on the growth rate. At low...
journal article 2016
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Lotti, T. (author)
Conventional wastewater treatment plants (WWTPs), like activated sludge systems, are energy demanding requiring a large electrical energy supply (e.g. 25 kWh PE-1 year-1) which, especially during peak-load periods, may account for an important quote of the grid installed power of the surrounding area. Only across the EU, there are 16000 WWTPs...
doctoral thesis 2016
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Milne, N.S.W. (author), Wahl, S.A. (author), Van Maris, A.J.A. (author), Pronk, J.T. (author), Daran, J.M. (author)
It is theoretically possible to engineer Saccharomyces cerevisiae strains in which isobutanol is the predominant catabolic product and high-yielding isobutanol-producing strains are already reported by industry. Conversely, isobutanol yields of engineered S. cerevisiae strains reported in the scientific literature typically remain far below 10%...
journal article 2016
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Mooij, P.R. (author)
This thesis deals with selective environments in microalgal cultivation. As explained in Chapter 1 microalgae have changed the course of life on Earth dramatically by performing oxygenic photosynthesis. In oxygenic photosynthesis electrons from water are used to reduce carbon dioxide to carbohydrates or lipids using solar energy. As a waste...
doctoral thesis 2016
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Heeres, A.S. (author)
doctoral thesis 2016
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Bolat, I. (author)
One of the best guarded secrets of brewers is represented by the brewing yeast employed in beer fermentation, due to its profound impact upon the specific flavour profile of the final product. The current research tackles the genome diversity of lager brewing strains as well as their impact on important phenotypic traits. Furthermore, the...
doctoral thesis 2016
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De Vries, S. (author), Dörner, K. (author), Strampraad, M.J.F. (author), Friedrich, T. (author)
Respiratory complex I converts the free energy of ubiquinone reduction by NADH into a proton motive force, a redox reaction catalyzed by flavin mononucleotide(FMN) and a chain of seven iron–sulfur centers. Electron transfer rates between the centers were determined by ultrafast freezequenching and analysis by EPR and UV/Vis spectroscopy. The...
journal article 2015
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De Almeida, N.M. (author), Neumann, S. (author), Mesman, R.J. (author), Ferousi, C. (author), Keltjens, J.T. (author), Jetten, M.S.M. (author), Kartal, B. (author), Van Niftrik, L. (author)
Anaerobic ammonium-oxidizing (anammox) bacteria oxidize ammonium with nitrite as the terminal electron acceptor to form dinitrogen gas in the absence of oxygen. Anammox bacteria have a compartmentalized cell plan with a central membrane-bound “prokaryotic organelle” called the anammoxosome. The anammoxosome occupies most of the cell volume, has...
journal article 2015
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Vos, T. (author), De la Torre Cortes, P. (author), Van Gulik, W.M. (author), Pronk, J.T. (author), Daran-Lapujade, P.A.S. (author)
Introduction: Saccharomyces cerevisiae has become a popular host for production of non-native compounds. The metabolic pathways involved generally require a net input of energy. To maximize the ATP yield on sugar in S. cerevisiae, industrial cultivation is typically performed in aerobic, sugar-limited fed-batch reactors which, due to constraints...
journal article 2015
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Gonzalez-Martinez, A. (author), Rodriguez-Sanchez, A. (author), Rodelas, B. (author), Abbas, B.A. (author), Martinez-Toledo, M.V. (author), Van Loosdrecht, M.C.M. (author), Osorio, F. (author), Gonzalez-Lopez, J. (author)
Identification of anaerobic ammonium oxidizing (anammox) bacteria by molecular tools aimed at the evaluation of bacterial diversity in autotrophic nitrogen removal systems is limited by the difficulty to design universal primers for the Bacteria domain able to amplify the anammox 16S rRNAgenes.Ametagenomic analysis (pyrosequencing) of total...
journal article 2015
Searched for: department%3A%22Biotechnology%22
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