Improved p-hydroxybenzoate production by engineered Pseudomonas putida S12 by using a mixed-substrate feeding strategy

The key precursors for p-hydroxybenzoate production by engineered Pseudomonas putida S12 are phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P), for which the pentose phosphate (PP) pathway is an important source. Since PP pathway fluxes are typically low in pseudomonads, E4P and PEP availability is a likely bottleneck for aromatics...

C5-technology in Pseudomonas putida S12: Construction, analysis and implementation of D-xylose metabolic pathways

The rising price of oil and impending deficit of fossil resources stimulate the development of alternative processes for the production of chemicals. The production of biochemicals from lignocellulosic biomass is a promising alternative. Lignocellulosic biomass consists of a mixture of sugars that can be converted into valuable products or...

Xylose anaerobic conversion by open-mixed cultures

Xylose is, after glucose, the dominant sugar in agricultural wastes. In anaerobic environments, carbohydrates are converted into volatile fatty acids and alcohols. These can be used as building blocks in biotechnological or chemical processes, e.g., to produce bioplastics. In this study, xylose fermentation by mixed microbial cultures was...

Diversity of microbial communities in open mixed culture fermentations: Impact of the pH and carbon source

Anaerobic fermentation by an open mixed culture was investigated at different pH values (4–8.5) and with three substrates (glucose, glycerol and xylose). The populations established in each condition were assessed by denaturing gradient gel electrophoresis analysis of the 16S ribosomal RNA gene fragments. The fermentation pattern and the...